Abstract
Young adult rats received either unilateral or bilateral ibotenic acid infusions in their nucleus basalis, destroying most of the cholinesterase-staining neurons in that region. Cerebral cortex levels of choline acetyltransferase, somatostatin, neuropeptide Y, and monoamines were then assayed 2.5 and 10 months after bilateral lesions, or, 2.5, 10, and 14 months after unilateral lesions. Entorhinal and cerebral cortex levels of several amino acid transmitters were also measured. As expected, choline acetyltransferase activity was decreased in the frontal cortex ipsilateral to the ibotenic acid infusion in unilaterally or bilaterally lesioned animals. Parietal cortex concentrations of somatostatin and neuropeptide Y were altered by lesioning in a complicated, time-dependent manner. Thus, while unilateral lesions transiently decreased or had no effect on these neuropeptide levels, bilateral lesions elevated the level of each neuropeptide by over 100% at 10 months. Other cortical transmitter systems investigated appeared to be less affected by nucleus basalis-lesions. Unilateral lesions had no effect on prefrontal cortex norepinephrine, serotonin, or dopamine content at 14 months post-lesioning. These different neurochemical effects of unilateral and bilateral nucleus basalis lesions may be important for developing a model for the trans-synaptic effects of cortical cholinergic deafferentation.
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References
Fibiger, H. C., 1982. The organization and some projections of cholinergic neurons of the mammalian forebrain.Brain Res. Rev. 4:327–388.
Bartus, R. T., Pontecorvo, M. J., Flicker, C., Dean, R. L., and Figueiedo, J. C. 1986. Behavioral recovery following bilateral lesions of the nucleus basalis does not occur spontaneously. Pharmacol. Biochem. Behav. 24:1287–1292.
Arendash, G. W., Strong, P. N., and Mouton, P. R. 1985. Intracerebral transplantation of cholinergic neurons in a new animal model for Alzheimer's disease. pp. 351–367, In:Senile Dementia of the Alzheimer Type, Edited by J. Hutton and A. Kenny, Alan R. Liss, Inc., New York.
Flicker, C., Dean, R. L., Watkins, D. L., Fisher, S. K. and Bartus, R. T. 1983. Behavioral and neurochemical effects following neurotoxic lesions of a major cholinergic input to the cerebral cortex of the rat.Pharmacol. Biochem. Behav. 18:973–981.
Knowlton, B. J., Wenk, G. L., Olton, D. S., and Coyle, J. T. 1985. Basal forebrain lesions produced dissociation of trial dependent and trial independent memory performance.Brain Res. 345:315–321.
Murray, C. L., and Fibiger, H. C. 1985. Learning and memory deficits after lesions of the nucleus basalis magnocellularis: reversal by physostigmine.Neuroscience 14:1025–1032.
Arendash, G. W., Millard, W. J., Dunn, A. J., and Meyer, E. M. 1987. Long-term neuropathological and chemical changes after lesions of the rat nucleus basalis magnocellularis.Science 238:952–956.
McKinney, M., Davies, P. and Coyle, J. T. 1982. Somatostatin is not co-localised in cholinergic neurons innervating the rat cerebral cortex-hippocampal functions.Brain Res 243:169–172.
Fine, A., Pittaway, K., De Quidt, M., Czudek, C. and Reynolds, G. P. 1987. Maintenance of cortical somatostatin and monoamine levels in the rat does not require intact cholinergic innervation.Brainn Res. 406:326–329.
Konig, J. and Klippel, A. 1963.The Rat Brain A Stereotaxic Atlas of the Forebrain and Lower Parts of the Brain Stem. William and Wilkins, Baltimore, MD.
Arendash, G. W. and Mouton, P. R. 1987. Transplantation of nucleus basalis magnocellularis cholinergic neurons into the cholinergic-depleted cerebral cortex: morphological and behavioral effects.Ann. N.Y. Acad. Sci. 495:431–443.
Roskoski, R. 1973. Choline acetyltransferasc. Evidence for an acetylenzyme reaction intermediate.Biochemistry 12:3709–3713.
Arnold, M. A., Reppert, S. M., Rorstad, O. P., Sagar, S. M., Keutman, H. T., Perlow, M. J. and Martin, J. B. 1982. Daily pattern of somatostatin in the cerebrospinal fluid of the rhesus monkey: effect of environmental lighting.J Neurosci 2:674–680.
Beal, M. F., Mazurek, M. F., Chattha, G. K., Svendsen, C. V., Bird, E. D. and Martin, J. B. 1986a. Neuropeptide Y immunoreactivity is reduced in cerebral cortex in Alzheimer's Disease.Annals of Neurology 20:282–28.
Beal, M. F., M. F. Mazurek, L. J. Lorenz, G. K., Chattha, D. W., Ellison, and J. B., Martin, 1986b. An, examination of neuropeptide Y post-mortem stability in animal model simulating human autopsy conditions.Neurosci Lett. 64:69–74.
Dunn, A. J. and Berridge, C. W. 1987. Corticotropin releasing factor administration elicits a stress-like activation of cerebral catecholaminergic systems.Pharmacol. Biochem. Behav. 27:685–691.
Bradford, M. 1976. A new technique for the determination of protein in small samples.Anal. Biochem. 72:248–254.
Montminy, M. R. and Bilezikjian, L. M. 1987. Binding of a nuclear protein to the cyclic AMP response element of the somatostatin gene.Nature 328:175–178.
Higuchi, H. and Sabol, S. L. 1987., Rat neuropeptide Y precursor mRNA: characterization, tissue distribution, and regulation by glucocorticoids, cyclic AMP, calcium and NGF.,17th Annual Society for Neurosciences Abstracts: 176.
Olianas, M. C., Onali, P., Neff, N. H. and Costa, E. 1983. Adenylate cyclase activity of synaptic membranes from rat striatum: inhibition by muscarinic agonists.Mol. Pharmacol. 23:393–398.
Meyer, E., arendash, G., Judkins, J., Ying, L., Wade, C., and Kem, W. 1987. Effects of Nucleus Basalis Lesions on the Muscarinic and Nicotinic Modulation of [3H]Acetylcholine Release in the Rat Cerebral Cortex.J. Neurochem. 49:1758–1762.
Potter, L. T., Flynn, D. D., Hanchett, H. E., Kalinoski, D. L., Luber-Narod, J. and Mash, D. C. 1984. Independent M1 and M2 receptors: Ligands, autoradiography and functions.Trends PHarmacol. Sci. 5 (Suppl.) 22–31.
Bartus, R. T., Flicker, C., Dean, R. L., Pontecorvo, M., Figueiredo, J. C. and Fisher, S. K. 1985. Selective memory loss following nucleus basalis lesions: long term behavioral recovery despite persistent cholinergic deficiencies.Pharmacol. Biochem. Behav. 23:125–135.
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Arendash, G.W., Millard, W.J., Dawson, R. et al. Different long-term effects of bilateral and unilateral nucleus basalis lesions on rat cerebral cortical neurotransmitter content. Neurochem Res 14, 1113–1118 (1989). https://doi.org/10.1007/BF00965617
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DOI: https://doi.org/10.1007/BF00965617