Abstract
3-((±)-2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP) is an antagonist at the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor. In the present study, levels of dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindolacetic acid (5-HIAA) were measured after intracerebroventricular injection of NMDA, CPP or both in rat striatum using a brain dialysis method. The injection of NMDA produced a significant increase in DOPAC level. HVA level was also increased by NMDA injection. The level of 5-HIAA was not affected by NMDA injection. The injection of CPP had no effect on DOPAC, HVA and 5-HIAA levels. The injection of CPP restrained the increase of DOPAC and HVA levels induced by NMDA injection. The results suggest that intracerebral injection of NMDA may increase dopamine release from rat striatum, but have no effect on serotonin release. Furthermore, CPP inhibits NMDA induced release of dopamine.
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Watkins, J. C. 1984. Excitatory amino acids and central synaptic transmission. Trends Pharmacol. Sci. 5:373–376.
Watkins, J. C., and Evans, R. H. 1981. Excitatory amino acid transmitters. Ann. Rev. Pharmacol. Toxicol. 21:165–204.
Kita, T., Kamiya, H., and Kiyota, C. 1963. Effect of intraventricular injection of N-methylated GABA-derivatives on the central nervous system of conscious mice. Biochem. Pharmacol. 12:213–221.
Anis, N. A., Berry, S. C., Burton, N. R., and Lodge, D. 1983. The dissociative anaesthetics, ketamine and phencyclidine, selectively reduce excitation of central mammalian neurones by N-methyl-aspartate. J. Pharmacol. 79:565–575.
Flatman, J. A., Schwindt, P. C., Crill, W. E., and Stafstrom, C. E. 1983. Multiple actions of N-methyl-D-aspartate on cat neocortical neurons in vitro. Brain Res. 266:169–173.
Gean, P. W., and Shinnick-Gallagher, P. 1988. Epileptiform activity induced by magnesium-free solution in slices of rat amygdala: Antagonism by N-methyl-D-aspartate receptor antagonists. Neuropharmacology 27:557–562.
Croucher, M. J., Collins, J. F., and Meldrum, B. S. 1982. Anticonvulsant action of excitatory amino acid antagonists. Science 216:889–901.
Meldrum, B. S., Croucher, M. J., Badman, G., and Collins, J. F. 1983. Antiepileptic action of excitatory amino acid antagonists in the photosensitive baboon, Papio papio. Neurosci. Lett. 39:101–104.
Chapman, A. G., Engelsen, B., and Meldrum, B. S. 1987. 2-Amino-7-phosphonoheptanoic acid inhibits insulin-induced convulsions and striatal aspartate accumulation in rats with frontal cortical ablation. J. Neurochem. 49:121–127.
Czuczwar, S. J. and Meldrum, B., 1982. Protection against chemically induced seizures by 2-amino-7-phosphonoheptanoic acid. Eur. J. Pharmacol. 83:335–338.
Peterson, D. W., Collins, J. F., and Bradford, H. F. 1983. The kindld amygdala model of epilepsy: anticonvulsant actions of amino acid antagonists. Brain Res. 275:169–172.
Simada, M., Kabuto, H., and Yokoi, I. 1987. The effect of ω-phosphono-α-aminocarboxylic acids on seizures and brain amino acid levels in E1 mice. Research Communications in Chemical Pathology and Pharmacology 57:359–373.
Herrling, P. L., Morris, R., and Salt, T. E. 1983. Effects of excitatory amino acids and their antagonists on membrane and action potentials of cat caudate neurons. J. Physiol. 339:207–222.
Davies, J., Evans, R. H., Herrling, P. L., Jones, A. W., Olverman, H. J., Pook, P., and Watkins, J. C. 1986. CPP, a new potent and selective NMDA antagonist. Depression of central neuron responses, affinity for [3H]D-AP5 binding sites on brain membranes and anticonvulsant activity. Brain Res. 382:169–173.
Harris, E. W., Ganong, A., Monaghan, D. T., Watkins, J. C., and Cotman, C. W. 1986. Action of 3-((±)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP): a new and highly potent antagonist of N-methyl-d-aspartate receptors in the hippocampus. Brain Res. 382:174–177.
Lehmann, J., Schneider, J., McPherson, S., Murphy, D. E., Bernard, P., Tsai, C., Bennett, D. A., Pastor, G., Steel, D. J., Boehm, C., Cheney, D. L., Liebman, J. M., Williams, M., and Wood, P. L. 1987. CPP, a selective N-methyl-d-aspartate (NMDA)-type receptor antagonist: characterization in vitro and in vivo. J. Pharmacol. Exp. Ther. 240:737–746.
Murphy, D. E., Schneider, J., Boehm, C., Lehmann, J., and Williams, M. 1987. Binding of [3H]3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid to rat brain membranes: a selective, high affinity ligand for N-methyl-d-aspartate receptors. J. Pharmacol. Exp. Ther. 240:778–784.
Turski, L., Klockgether, T., Sontag, K. H., Herrling, P. L., and Watkins, J. C. 1974. Muscle relaxant and anticonvulsant activity of 3-((±)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid, a novel N-methyl-d-aspartate antagonist, in rodents. Neurosci. Lett. 73:143–148.
Boldry, R. C., and Uretsky, N. J. 1988. The importance of dopaminergic neurotransmission in the hypermotility response produced by the administration of N-methyl-d-aspartic acid into the nucleus accumbens. Neuropharmacology 27:569–577.
Jones, S. M., Snell, L. D., and Johnson, K. M. 1987. Inhibition by phencyclidine on excitatory amino acid-stimulated release of neurotransmitter in the nucleus accumbens. Neuropharmacology 26:173–179.
Roberts, P. J., and Anderson, S. D. 1979. Stimulatory effect ofl-glutamate and related amino acids on [3H]dopamine release from rat striatum: an in vitro model for glutamate actions. J. Neurochem. 32:1539–1545.
Roberts, P. J., and Sharif, N. A. 1978. Effects ofl-glutamate and related amino acids upon the release of [3H]dopamine from rats striatal slices. Brain Res. 157:391–395.
Snell, L. D., and Johnson, K. M. 1986. Characterization of the inhibition of excitatory amino acid-induced neurotransmitter release in the rat striatum by phencyclidine-like drugs. J. Pharmacol. Exp. Ther. 238:938–946.
Baker, M. K., Jenner, P., and Marsden, C. D. 1985. Excitatory amino acid agonists and antagonists alter K+-evoked, but not basal, release of [3H]-dopamine from rat striatal slices. Br. J. Pharmac. 84:181.
Phebus, L. A., Perry, K. W., Clemens, J. A., and Fuller, R. W. 1986. Brain anoxia releases striatal dopamine in rats. Life Sci. 38:2447–2453.
Kaneyuki, T., Morimasa, T., and Shohmori, T. 1986. Changes in brain monoamine levels in cholecystokinin-induced satiety. Neurosciences 12:104–105.
Lasley, S. M., Michaelson, I. A., Greenland, R. D., and McGinnis, P. M. 1984. Simultaneous measurement of tyrosine, tryptophan and related monoamines for determination of neurotransmitter turnover in discrete rat brain regions by liquid chromatography with electrochemical detection. J. Chromatogr. 305:27–42.
Divac, I., Fonnun, F. and Storm-Mathisen, J. 1977. High-affinity uptake of glutamate in terminals of corticostriatal axons. Nature (Lond) 266:377–378.
McGeer, P. L., McGeer, E. G., Scherer, U., and Singh, K. 1977. A glutamatergic corticostriatal path?. Brain Res. 128:369–373.
Gioguieff, M. F., Kemel, M. L., and Glowinski, J. 1977. Presynaptic effect ofl-glutamic acid on the release of dopamine in the striatal slices. Neurosci. Lett. 6:73–78.
Lehmann, J. and Scatton, B. 1982. Characterization of excitatory amino acid receptor-mediated release of [3H]acetylcholine from rat striatal slices. Brain Res. 252:77–89.
Herdon, H., Strupish, J., and Nahorski, S. R. 1985. Differences between the release of radiolabelled and endogenous dopamine from superfused rat brain slices: effects of depolarizing stimuli, amphetamine and synthesis inhibition. Brain Res. 348:309–320.
McBean, G. J., and Roberts, P. J. 1981. Glutamate-preferring receptors regulate the release ofd-[3H]-aspartate from rat hippocampal slices. Nature (Lond) 291:593–594.
Wojtowicz, J. M., Gysen, M., and MacDonald, J. F. 1981. Multiple reversal potentials for responses tol-glutamic acid. Brain Re. 213:195–200.
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Kabuto, H., Yokoi, I., Mizukawa, K. et al. Effects of an N-methyl-d-aspartate receptor agonist and its antagonist CPP on the levels of dopamine and serotonin metabolites in rat striatum collected in vivo by using a brain dialysis technique. Neurochem Res 14, 1075–1080 (1989). https://doi.org/10.1007/BF00965613
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DOI: https://doi.org/10.1007/BF00965613