Abstract
The binding [125I]insulin and its displacement by the unlabeled hormone was measured in vitro in the isolated cerebral capillaries and astroglia cell-enriched fraction as well as in the particulate fraction of cerebral homogenate and liver plasma membrane preparation. The results indicate the specificity and affinity of the hormone binding to astrocytes to be similar to that to cerebral homogenate and liver fractions and markedly higher than to cerebral blood vessels. The possible functional significance of the insulin receptors on astrocytes is discussed.
Similar content being viewed by others
References
Havankova, J., Schmechel, D., Roth, J., andBrownstein, M. 1978. Identification of insulin in rat brain. Proc. Natl. Acad. Sci. 75:5737–5741.
Rozenweig, J. I., Havrankova, J., Leśniak, M. A., Brownstein, M., andRoth, J. 1980. Insulin is ubiquitous in extrapancreatic tissue of rats and humans. Proc. Natl. Acad. Sci. 77:572–576.
Van Houten, M., andPosner, B. I. 1979. Insulin binds to brain vessels in vivo. Nature, 282:623–625.
Van Houten, M., Posner, B. I., Kaptiwa, B. M., andBrower, J. R. 1979. Insulinbinding sites in the rat brain: In vivo localization to the circumventricular organs by quantitative radioautography. Endocrinol. 105:666–673.
Posner, B. I., Kelly, P. A., Shin, R. P. C., andFrisen, H. G. 1974. Studies of insulin, growth hormone and prolactin binding: Tissue distribution, species variation and characterization. Endocrinol. 95:521–531.
Havrankova, J., Roth, J., andBrownstein, M. 1978. Insulin receptors are widely distributed in the central nervous system of the rat. Nature 272:827–829.
Goldstein, G. W., Wolinsky, J. S., Cscejtey, J., andDiamond, J. 1975. Isolation of metabolically active capillaries from rat brain. J. Neurochem. 25:715–717.
Farooq, M., andNorton, W. T. 1978. A modified procedure for isolation of astrocyte-and neuron-enriched fractions from rat brain. J. Neurochem. 31:887–894.
Albrecht, J., Hilgier, W., Ułas, J., andWysmyk-Cybula, U. 1982. Some properties of a “crude” fraction of astrocytes prepared with trypsin. Neurochem. Res. 7:513–518.
Goldfine, I. D., Amir, S. M., Peterson, A. W., andIngbar, S. H. 1974. Preparation of biologically active125I-TSH. Endocrinol. 95:1228–1233.
Lowry, O. H., Rosenbrough, H. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Henn, F. A., Deering, J., andAnderson, D. 1980. Receptor studies on isolated astroglial cell fractions prepared with and without trypsin. Neurochem. Res. 5:459–463.
Goodner, C. J., andBerrie, M. A. 1974. The failure of rat hypothalamic tissues to take up labeled insulin in vivo or to respond to insulin in vitro. Endocrinol. 101:605–610.
White, E. P. 1980. The synthesis and possible transport of specific proteins by cells associated with brain capillaries. J. Neurochem. 35:88–94.
Sönksen, P. H. 1979. Probing the insulin receptor. Nature 282:11–12.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Albrecht, J., Wróblewska, B. & Mossakowski, M.J. The binding of insulin to cerebral capillaries and astrocytes of the rat. Neurochem Res 7, 489–494 (1982). https://doi.org/10.1007/BF00965500
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00965500