Abstract
The effect of hydrocortisone and thyroxine, on the activities of Ca2+-and Mg2+-ATPase was studied in cultured neuronal (clone M1) and glial (clones NN and C6) cell lines. For M1 and NN cells an increase in Ca2+-and Mg2+-ecto-ATPase activity was found when the cells were cultured during 4–6 days in presence of hydrocortisone or together with thyroxine. In the same conditions, a decrease in Ca2+-and Mg2+-ecto-ATPase activity was found for the C6 cells. In C6 cells the effect of hormones was more pronounced for the Mg2+-than for the Ca2+-ecto-ATPase activity. The observed decrease may be related to the tumoral origin of the C6 cells. The activity of (Na+, K+)-ATPase in all three cell lines increased in presence of hydrocortisone or together with thyroxine when the cells were cultured during 4–6 days, in presence of the hormones, whereas the total Mg2+-ATPase activity increased only after 6 days of treatment. Thyroxine alone has very few effect either on Ca2+-and Mg2+-ecto-ATPase, or on (Na+, K+)-and total Mg2+-ATPase activity. These observations are interpreted to indicate that hormones may modulate or induce enzymatic activities involved in active transport phenomena in nervous tissue.
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References
Lissack, K. 1971. Hormones and Brain Function, inLissack (ed.), Plenum Press, New York.
O'Malley, B. W., andSchrader, W. T. 1976. The receptors of steroid hormones. Scient. America 234:32–43
McEwen, B. S. 1976. Interactions between hormones and nerve tissue. Scient. America 235:48–58.
De Vellis, J., andBrooker, G. 1973. Tissue Culture of the Nervous System. Pages 231,in Sato, G. (ed.), Academic Press, New York.
Alescio, T., andMoscona, A. A. 1969. Immunochemical evidence for enzyme synthesis in the hormonal induction of glutamine synthetase in embryonic retina in culture. Biochem. Biophys. Res. Commun. 34:176–182.
Woodbury, D. M., Timiras, P. S., andVernadakis, A. 1957. Hormone, Brain Function and Behavior, Pages 27,in Hoagland, H. (ed.), Academic Press, New York.
Tata, J. R. 1957. Brain Functions and Behavior, Pages 197,in Hoaglund, H. (ed.), Academic Press, New York.
Hamlyn, J. M., andDuffy, T. 1978. Direct stimulation of human erythrocyte membrane (Na+, K+)-Mg2+-ATPase activity in vitro by physiological concentrations of d-Aldosterone. Biochem. Biophys. Res. Comm. 84:458–464.
Ming, H. L., andTai, A. 1978. Increased (Na+, K+)-ATPase concentrations in various tissues of rats caused by thyroid hormone treatment. J. Biol. Chem. 253:723–726.
Massa, E. M., Moreto, R. D., Bloj, B., andFarias, R. N. 1975. Hormone action and membrane fluidity: effect of insuline and cortisone on the Hill coefficients of rat erythrocyte membrane-bound acetylcholinesterase and (Na+, K+)-ATPase. Biochem. Biophys. Res. Commun. 66:115–122.
Dahl, J. L., andHokin, L. E. 1974. The sodium-potassium adenosine triphosphatase. Ann. Rev. Biochem. 43:327–356.
Stefanovic, V., Ledig, M., andMandel, P. 1976. Divalent cation activated ecto nucleoside triphosphatase activity of nervous system cells in tissue culture. J. Neurochem. 27:799–805.
Shein, H. M., Britva, A., Hess, H. H., andSelkoe, D. J. 1970. Isolation of hamster brain astroglia by in vitro cultivation and subcutaneous growth, and content of cerebroside, ganglioside, RNA and DNA. Brain Res. 19:497–501.
Benda, P., Lightbody, J., Sato, G., Levine, L., andSweet, W. 1968. Differentiated rat glial cell strains in tissue culture. Science 161:370.
Ciesielski-Treska, J., Warter, S., andMandel, P. 1975. Morphological histochemical and chromosomal patterns of neuroblastoma parental and purine resistant lines. Neurobiology 5:382–392.
Stefanovic, V., Ciesielski-Treska, J., Ebel, A., andMandel, P. 1974. Nucleoside triphosphate activity at the external surface of neuroblastoma cells. Brain Res. 81:427–441.
Gomori, G. 1942. A modification of the colorimetric phosphorus determination for use with the photoelectric colorimeter. J. Lab. Clin. Med. 27:955–960.
Weil-Malherbe, H., andGreen, R. H. 1951. The catalytic effect of molybdate on the hydrolysis of organic phosphate bonds. Biochem. J. 49:286–292.
Le Deaut, J. Y., Ledig, M., andMandel, P. 1976. Etude comparative du dosage du phosphate inorganique libéré après action d'une ATPase. Biochimie 58:1397–1399.
Lowry, O. H., Rosebrought, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Berliner, J. A., Bennett, K., andDe Vellis, J. 1978. Effect of hydrocortisone on cell morphology in C6 cells. J. Cell Physiol. 94:321–334.
Grasso, R. J. 1976. Transient inhibition of cell proliferation in rat glioma C6 cells by cortisol. Cancer Res. 36:2408–2414.
Grasso, R. J., andJohnson, C. E. 1977. Dose response relationship between glucocorticoids and growth inhibition in rat glioma monolayer culture. Proc. Soc. Expt. Biol. Med. 154:238–241.
Grasso, R. J., Tedesco, T. A., Wodzinski, S. F., andJohnson, C. 1978. Temporal relationships between DNA metabolism and the growth inhibitory response produced by dexamethasone in rat glioma cell cultures. In Vitro 14:625–630.
Vernadakis, A. 1971. Hormonal factors in the proliferation of glial cells in culture in influence of hormonal factors in the proliferation of glial cells in culture. Pages 42–55,in Karger, (ed.), Proc. Int. Soc. Psychoneuroendocrinology, Basel.
De Vellis, J., Inglish, D., Cole, R., andMolson, J. 1971. Effect of hormones on the differentiation of cloned lines of neurones and glial cells. Pages 26–39,in Karger, (ed.), Proc. Int. Soc. Psychoneuroendocrinology, Basel.
Ledig, M., Ciesielski-Treska, J., Cam, Y., Montagnon, D., andMandel, P. 1975. ATPase activity of neuroblastoma cells in culture. J. Neurochem. 25:635–640.
Huttenlocher, P. R., andAmemiya, I. M. 1978. Effect of adrenocortical steroids and of adrenocorticotrophichormone on (Na+, K+)-ATPase in immature cerebral cortex. Pediat. Res. 12:104–107.
Tamasdan, S., Nuta, G., andMiehele, D. 1977. The immediate effect of corticosteroid hormones on nucleic acids and on the magnesium-sodium-and potassium-dependent adenosine triphosphatase in the liver. Farmacia (Bucharest) 25:201–205.
Lazarewicz, J. W., Kanje, M., Sellström A., andHamberger, A. 1977. Calcium fluxes in cultured and bulk isolated neuronal and glial cells. J. Neurochem. 29:495–502.
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Elkouby, A., Ledig, M. & Mandel, P. Effect of hydrocortisone and thyroxine on ATPase activities of neuronal and glial cell lines in culture. Neurochem Res 7, 387–397 (1982). https://doi.org/10.1007/BF00965492
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DOI: https://doi.org/10.1007/BF00965492