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Amino acid incorporation in relation to molecular weight of proteins in young and adult brain

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Abstract

Rates of protein synthesis were studied in immature and adult rat brain tissue. After an amino acid incorporation period, in vivo or in incubated slices from brain, the soluble protein was fractionated according to molecular weight by column chromatography. In examining soluble whole proteins, no direct correlation between molecular weights and synthesis rates could be established; the highest synthesis rates were found in fractions around 70,000 MW and below 10,000. Incorporation into the subunits after fractionation by SDS gel electrophoresis was proportional to subunit molecular weight, with rates of incorporation into the largest subunits being the highest. The results suggest a relationship between turnover rate and structure of subunits of brain proteins.

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Authors and Affiliations

  1. Center for Neurochemistry, The Nathan S. Kline Institute for Psychiatric Research, Ward's Island, 10035, New York, NY

    F. M. Shahbazian & Abel Lajtha

  2. Department of Pathobiology and Oral Pathology College of Denistry, New York University, 10010, New York, NY

    Myron Jacobs

Authors
  1. F. M. Shahbazian
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  2. Myron Jacobs
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  3. Abel Lajtha
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Shahbazian, F.M., Jacobs, M. & Lajtha, A. Amino acid incorporation in relation to molecular weight of proteins in young and adult brain. Neurochem Res 11, 647–660 (1986). https://doi.org/10.1007/BF00965334

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