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Anti-human choline acetyltransferase Fragments Antigen Binding (FAB)-Sepharose chromatography for enzyme purification

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Abstract

Fragments Antigen Binding (FAB)-Sepharose immunoaffinity chromatography was successfully applied to the purification of human choline acetyltransferase. The mild elution conditions made it possible to obtain the enzyme in its full catalytic state. The method should be generally applicable to enzyme purification.

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Authors and Affiliations

  1. Kinsmen Laboratory of Neurological Research Department of Psychiatry, University of British Columbia, 2255 Wesbrook Mall, V6T 1W5, Vancouver, B. C., Canada

    J. H. Peng, P. L. McGeer & E. G. McGeer

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  1. J. H. Peng
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  2. P. L. McGeer
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  3. E. G. McGeer
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Peng, J.H., McGeer, P.L. & McGeer, E.G. Anti-human choline acetyltransferase Fragments Antigen Binding (FAB)-Sepharose chromatography for enzyme purification. Neurochem Res 8, 1481–1486 (1983). https://doi.org/10.1007/BF00965003

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  • DOI: https://doi.org/10.1007/BF00965003

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