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The stokes radius of the chapssolubilized benzodiazepine receptor complex

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Abstract

The Stokes radii and the apparent molecular weights of the CHAPS or Triton X-100 solubilized benzodiazepine receptors are calculated by gel exclusion chromatography. The results suggest that the molecular receptor complex solubilized by CHAPS is much larger than the complex solubilized by Triton X-100.

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References

  1. Lang, B., Barnard, E. A., Chang, L. R., andDolly, J. O. 1979. Putative benzodiazepine receptor: A protein solubilized from brain. FEBS lett. 104:149–153.

    Google Scholar 

  2. Gavish, M., andSnyder, S. H. 1980. Soluble benzodiazepine receptors: GABAergic regulation. Life Sci 26:579–582.

    Google Scholar 

  3. Gavish, M., andSnyder, S. H. 1981. γ-Aminobutyric acid and benzodiazepine receptors: Copurification and characterization. Proc. Natl. Acad. Sci. USA 78:1939–1942.

    Google Scholar 

  4. Massotti, M., Guidotti, A., andCosta, E. 1981. Characterization of benzodiazepine and γ-aminobutyric recognition sites and their endogenous modulators. J. Neurosc. 1:409–418.

    Google Scholar 

  5. Lo, M. M. S., Strittmatter, S. M., andSnyder, S. H. 1982. Physical separation and classification of two types of benzodiazepine receptors. Proc. Natl. Acad. Sci. USA 79:680–684.

    Google Scholar 

  6. Martini, C., Lucacchini, A., Ronca, G., Hrelia, S., andRossi, C. A. 1982. Isolation of putative benzodiazepine receptors from rat brain membranes by affinity chromatography. J. Neurochem. 38:15–19.

    Google Scholar 

  7. Yousufi, M. A. K., Thomas, J. W., andTallman, J. F. 1979. Solubilization of benzodiazepine binding site from rat cortex. Life Sci. 25:463–470.

    Google Scholar 

  8. Sherman-Gold, R., andDudai, Y. 1980. Solubilization and properties of a benzodiazepine receptor from calf cortex. Brain Res. 198:485–490.

    Google Scholar 

  9. Asano, T., andOgasawara, N. 1981. Soluble gamma-aminobutyric acid and benzodiazepine receptors from rat cerebral cortex. Life Sci. 29:193–200.

    Google Scholar 

  10. Stephenson, T. A., Watkins, A. E., andOlsen, R. W. 1982. Physiochemical characterization of detergent-solubilized γ-aminobutyric acid and benzodiazepine receptor proteins from protein brain. Eur. J. Biochem., 123:291–298.

    Google Scholar 

  11. Siegel, E., Stephenson, F. A., Mamalaki, C., andBarnard, E. A. 1983. A γ-Aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex. J. Biol. Chem. 258:6965–6971.

    Google Scholar 

  12. Stephenson, F. A., andOlsen, R. W. 1982. Solubilization by CHAPS detergent of barbiturate-enhanced benzodiazepine-GABA receptor complex. J. Neurochem. 39:1579–1586.

    Google Scholar 

  13. Mernoff, S. T., Cherwinski, H. M., Becker, J. W., andDe Blas, A. L. 1983. Solubilization of brain benzodiazepine receptors with a zwitterionic detergent: Optimal preservation of their functional interaction with the GABA receptor. J. Neurochem., 41:752–758.

    Google Scholar 

  14. Siegel, E., andBarnard, E. A. 1984. A γ-Aminobutyric acid/benzodiazepine receptor complex from bovine cerebral cortex. J. Biol. Chem. 259:7219–7223.

    Google Scholar 

  15. Olsen, R. W., 1981. GABA-benzopdiazepine-barbiturate receptor interactions. J. Neurochem., 37:1–13.

    Google Scholar 

  16. Richards, J. G., andMöhler, H. 1984. Benzodiazepine receptors. Neuropharmacology, 23:233–242.

    Google Scholar 

  17. Ticku, M. K. 1983. Benzodiazepine-GABA receptor-ionophore complex. Current concepts. Neuropharmacology, 22:1459–1470.

    Google Scholar 

  18. Turner, A. J., andWhittle, S. R. 1983. Biochemical dissection of the γ-aminobutyrate synapses. Biochem. J., 209:29–41.

    Google Scholar 

  19. Guidotti, A., Corda, M. G., Wise, B. C., Vaccarino, F., andCosta, E. 1983. GABAergic synapses. Supramolecular organization and biochemical regulation. Neuropharmacology, 22:1471–1479.

    Google Scholar 

  20. Möhler, H., Battersby, M. K., andRichards, J. G. 1980. Benzodiazepine receptor protein identified and visualized in brain tissue by a photoaffinity label Proc. Natl. Acad. Sci. USA, 77:1666–1670.

    Google Scholar 

  21. Sieghart, W., andKarobath, M. 1980. Molecular heterogeneity of benzodiazepine receptors. Nature, 286,285–287.

    Google Scholar 

  22. Thomas, J. W., andTallman, J. F. 1981. Characterization of photoaffinity labeling of benzodiazepine binding sites. J. Biol. Chem., 256:9838–9842.

    Google Scholar 

  23. Chang, L.-R., andBarnard, E. A. 1982. The benzodiazepine/GABA receptor complex: Molecular size in brain synaptic membranes and in solution. J. Neurochem. 39:1507–1518.

    Google Scholar 

  24. Greenlee, D. V., andOlsen, R. W. 1979. Solubilization of gamma-aminobutyric acid receptor protein for mammalian brain. Biochem. Biophys. Res. Comm., 88:380–387.

    Google Scholar 

  25. Paul, S. M., Kempner, E. S., andSkolnick, P. 1981. In situ molecular weight determination of brain and peripheral benzodiazepine binding sites. Eur. J. Pharmacol. 76:465–466.

    Google Scholar 

  26. Doble, A., andIversen, L. L. 1982. Molecular size of benzodiazepine receptor in rat brain in situ: Evidence for a functional dimer. Nature, 295:522–523.

    Google Scholar 

  27. Laemmli, U. K. 1979. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227:680–685.

    Google Scholar 

  28. Laskey, R. A., andMills, A. D. 1975. Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur. J. Biochem., 56:335–341.

    Google Scholar 

  29. Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  30. McEnery, M. W., Buhle, Jr., E. L., Aebi, U., andPedersen, P. L. 1984. Protein ATPase of rat liver mitochondria. J. Biol. Chem. 259:4642–4651.

    Google Scholar 

  31. Wagner, S. L., Dean, W. L. andGay, R. D. 1984. Effect of a zwitterionic detergent on the state of aggregation and catalytic activity of cytochrome P-45OLM2 and NADPH-cytochrome P-450 reductase. J. Biol. Chem., 259:2390–2395.

    Google Scholar 

  32. Clarke, S. 1975. The size and detergent binding of membrane proteins. J. Biol. Chem., 250:5459–5469.

    Google Scholar 

  33. Tanford, C., andReynolds, J. 1976. Characterization of membrane proteins in detergent solutions. Biochem. Biophys. Acta, 457:133–170.

    Google Scholar 

  34. Helenius, A., andSimons, K. 1975. Solubilization of membranes by detergents. Biochem. Biophys. Acta, 415:29–79.

    Google Scholar 

  35. Hjelmeland, L. M., Nebert, D. W., andOsborne, J. C. 1983. Sulfobetaine derivatives of bile acids: Nondenaturing surfactants for membrane biochemistry. Anal. Biochem., 130:72–82.

    Google Scholar 

  36. Nozaki, Y., Schechter, N. M., Reynolds, J. A., andTanford, C. 1976. Use of gel chromatography for the determination of the Stokes Radius of proteins in the presence and absence of detergents. A reexamination. Biochem., 15:3884–3890.

    Google Scholar 

  37. Le Maire, M., Rivas, E. andMoller, J. V., 1980. Use of chromatography for determination of size and molecular weight of proteins: Further caution. Anal. Biochem., 106:12–21.

    Google Scholar 

  38. Reynolds, J. A., andTanford, C. 1976. Determination of molecular weight of the protein moiety in protein-detergent complexes without direct knowledge of detergent binding. Proc. Natl. Acad. Sci. USA 73:4467–4470.

    Google Scholar 

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Ray, J.P., Mernoff, S.T., Sangameswaran, L. et al. The stokes radius of the chapssolubilized benzodiazepine receptor complex. Neurochem Res 10, 1221–1229 (1985). https://doi.org/10.1007/BF00964841

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