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In vivo acylation of proteolipid protein and DM-20 in myelin and myelin subfractions of developing rat brain: Immunoblot identification of acylated PLP and DM-20

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Abstract

The acylation of proteolipid protein (PLP) was examined in myelin and myelin subfractions from rat brain during the active period of myelination. Proteolipid protein and DM-20 in myelin and myelin subfractions were readily acylated in developing rat brain 22 hours after intracerebral injection of [3H]palmitic acid. No differences in the relative specific activity of PLP in myelin from 9-, 15-, and 30-day-old rat brains was observed; however, the relative specific activity of PLP in the heavy myelin subfraction tended to be higher than that in the light myelin subfraction. The acylation of PLP was confirmed by fluorography of immunostained cellulose nitrate sheets, clearly establishing that the acylated protein is in fact the oligodendroglial cell- and myelin-specific protein, PLP. Since PLP is acylated in the 9-day-old animal, when little compact myelin is present, it is possible that the acylation of PLP is a prerequisite for the incorporation of this protein into the myelin membrane.

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Authors and Affiliations

  1. Department of Pediatrics, Washington University School of Medicine, 500 S. Kingshighway, P.O. Box 14871, 63178, St. Louis, Missouri

    Margaret M. Garwood, William R. Gilbert & Harish C. Agrawal

  2. Department of Neurology, Washington University School of Medicine, 500 S. Kingshighway, P.O. Box 14871, 63178, St. Louis, Missouri

    Harish C. Agrawal

  3. Department of Neuropathology, Washington University School of Medicine, 500 S. Kingshighway, P.O. Box 14871, 63178, St. Louis, Missouri

    Harish C. Agrawal

Authors
  1. Margaret M. Garwood
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  2. William R. Gilbert
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  3. Harish C. Agrawal
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Garwood, M.M., Gilbert, W.R. & Agrawal, H.C. In vivo acylation of proteolipid protein and DM-20 in myelin and myelin subfractions of developing rat brain: Immunoblot identification of acylated PLP and DM-20. Neurochem Res 8, 649–659 (1983). https://doi.org/10.1007/BF00964704

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  • DOI: https://doi.org/10.1007/BF00964704

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