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Developmental change of endogenous glutamate and gamma-glutamyl transferase in cultured cerebral cortical interneurons and cerebellar granule cells, and in mouse cerebral cortex and cerebellum in vivo

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Abstract

The developmental change of endogenous glutamate, as correlated to that of gamma-glutamyl transferase and other glutamate metabolizing enzymes such as phosphate activated glutaminase, glutamate dehydrogenase and aspartate, GABA and ornithine aminotransferases, has been investigated in cultured cerebral cortex interneurons and cerebellar granule cells. These cells are considered to be GABAergic and glutamatergic, respectively. Similar studies have also been performed in cerebral cortex and cerebellum in vivo. The developmental profiles of endogenous glutamate in cultured cerebral cortex interneurons and cerebellar granule cells corresponded rather closely with that of gamma-glutamyl transferase and not with other glutamate metabolizing enzymes. In cerebral cortex and cerebellum in vivo the developmental profiles of endogenous glutamate, gamma-glutamyl transferase and phosphate activated glutaminase corresponded with each other during the first 14 days in cerebellum, but this correspondence was less good in cerebral cortex. During the time period from 14 to 28 days post partum the endogenous glutamate concentration showed no close correspondence with any particular enzyme. It is suggested that gamma-glutamyltransferase regulates the endogenous glutamate concentration in culture neurons. The enzyme may also be important for regulation of endogenous glutamate in brain in vivo and particularly in cerebellum during the first 14 days post partum. Gamma-glutamyl transferase in cultured neurons and brain tissue in vivo appears to be devoid of maleate activated glutaminase.

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Abbreviations

Asp-T:

aspartate aminotransferase (EC 2.6.1.1)

GABA-T:

GABA aminotransferase (EC 2.6.1.19)

GAD:

glutamate decarboxylase (EC 4.1.1.15)

gamma-GT:

gamma-glutamyl transferase (gamma-glutamyl transpeptidase) (EC. 2.3.2.2)

Glu:

glutamate

GDH:

glutamate dehydrogenase (EC 1.4.1.3)

GS:

glutamine synthetase (EC 6.3.1.2)

MAG:

maleate activated glutaminase

Orn-T:

ornithine aminotransferase (EC 2.6.1.13)

PAG:

phosphate activated glutaminase (EC 3.5.1.1)

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Authors and Affiliations

  1. Neurochemical Laboratory, University of Oslo Preclinical Medicine, Blindern, P.O. Box 1115, Oslo 3, Norway

    Elling Kvamme, Ingeborg Aa. Torgner & Gerd Svenneby

  2. Department of Biochemistry A Panum Institute, University of Copenhagen, DK-2200, Denmark

    Arne Schousboe

  3. Department of Pharmacology, University of Saskatchewan, S7N OWO, Saskatoon, Saskatchewan, Canada

    Leif Hertz

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  1. Elling Kvamme
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  2. Arne Schousboe
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  4. Ingeborg Aa. Torgner
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Kvamme, E., Schousboe, A., Hertz, L. et al. Developmental change of endogenous glutamate and gamma-glutamyl transferase in cultured cerebral cortical interneurons and cerebellar granule cells, and in mouse cerebral cortex and cerebellum in vivo. Neurochem Res 10, 993–1008 (1985). https://doi.org/10.1007/BF00964635

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