Abstract
Sera from patients with multiple sclerosis (MS) and those obtained from normal subjects are indistinguishable by regular 5% or 7% polyacrylamide gel electrophoresis. However, 11 out of 15 MS sera examined by gradient polyacrylamide gel electrophoresis showed three distinct protein bands. None of the sera obtained from 10 normal subjects showed the characteristic protein patterns when they were examined by gradient gel electrophoresis. Similar results were obtained with de-albumin serum samples or with serum proteins precipitable at 50% ammonium sulfate saturation. These three proteins have now been purified to homogeneity by preparative gradient gel electrophoresis. Molecular weights of these proteins were estimated from gradient gel electrophoresis as 398,000, 363,000, and 302,000 daltons, respectively.
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Refences
Seil, F. J. 1977. Tissue culture studies of demyelinating disease: A critical review. Ann. J. Neurol. 2:345–355.
Paty, D. W. 1977. Multiple sclerosis: Recent advances in diagnosis, clinical immunology and virology. Can. Med. Assoc. J. 117:113–114.
Abramsky, O., Lisak, R. P., Silberger, D. H., andPleasure, D. E. 1977. Antibodies of oligodendroglia in patients with multiple sclerosis. N. Engl. J. Med. 297:1207–1211.
Chou, C.-H. J., Chou, F. C.-H., Kowalski, T. J., Kowalski, R., andKibler, R. F. 1978. Human myelin basic protein: Discovery of a genetic variant. Trans. Am. Soc. Neurochem. 9:106.
Braun, P. E., Carson, J. H., Barbarese, E., andMcPherson, T. A. 1978. Components in MS CSF that are detected by RIA for myelin basic protein. Trans. Am. Soc. Neurochem. 9:60.
Ueno, K., Glaser, G. H., andYu, R. K. 1978. Biochemical study on multiple sclerosis spinal cord. Trans. Am. Soc. Neurochem. 9:163.
Banik, N., Carnes, J., Mauldin, L., andHogan, E. L. 1978. Activity of cyclic nucleotide phosphohydrolase in human CSF. Trans. Am. Soc. Neurochem. 9:141.
Delmotte, P., andGonsette, R. 1977. Biochemical findings in multiple sclerosis. IV. Isoelectric focusing of the CSF gamma globulins in multiple sclerosis (262 cases) and other neurological diseases (272 cases). J. Neurol. 215:27–37.
Trotter, J. L., Banks, G., andWang, P. 1977. Isoelectric focusing of gamma globulin in cerebrospinal fluid from patients with multiple sclerosis. Clin. Chem. 23:2213–2215.
Johnson, K. P., andNelson, B. J. 1977. Multiple sclerosis: Diagnostic usefulness of cerebrospinal fluid. Ann. Neurol. 2:425–431.
Bornstein, M. B., andAppel, S. H. 1961. The application of tissue culture to the study of experimental “allergic” encephalomyelitis. I. Patterns of demyelination. J. Neuropathol. Exp. Neurol. 20:141–157.
Bornstein, M. B., andCrain, S. M. 1965. Functional studies of cultured brain tissues as related to “demyelinative disorder”, Science 148:1242–1244.
Kies, M. W., Driscoll, B. F., Seil, F. J., andAlvord, E. C. 1973. Myelination inhibition factor: Dissociation from induction of experimental allergic encephalomyelitis. Science 179:689–690.
Kim, S. U., Murray, M. R. Tourtellotte, W. W., andParker, J. A., 1970. Analysis of myelinotoxic factors in human disease. J. Neuropathol. Exp. Neurol. 29:147.
Travis, J., andPannell, R. 1973. Selective removal of albumin from plasma by affinity chromatography. Clin. Chim. Acta 49:49–52.
Wille, L. E. 1976. A simple and rapid method for isolation of serum lipoproteins from the other serum protein constituents. Clin. Chim. Acta 71:355–357.
Lorentz, K. 1976. A simple polyacrylamide gradient gel preparation for estimating molecular weights. Anal. Biochem. 76:214–220.
Gabridge, M. G., Singer, S. E., andEsposito, R. A. 1976. Gradient polyacrylamide gel electrophoresis of proteins from cytotoxic mycoplasma membranes. Biochem. Biophys. Res. Commun. 70:271–279.
Saito, K., Barber, R., Wu, J.-Y., Matsuda, T., Roberts, E., andVaughn, J. E. 1974. Immunohistochemical localization of glutamic acid decarboxylase in rat cerebellum. Proc. Natl. Acad. Sci. U.S.A. 71:269–273.
McLaughlin, B. J., Wood, J. G., Saito, K. Barber, R., Vaughn, J. E., Roberts, E., andWu, J.-Y. 1974. The fine structural localization of glutamate decarboxylase in synaptic terminals of rodent cerebellum. Brain Res. 76:377–391.
McLaughlin, B. J., Barber, R., Saito, K., Roberts, E., andWu, J.-Y. 1975. Immunocytochemical localization of glutamate decarboxylase in rat spinal cord. J. Comp. Neurol. 164:305–321.
McLaughlin, B. J., Wood, J. G., Saito, K., Roberts, E., andWu, J.-Y. 1975. The fine structural localization of glutamate decarboxylase in developing axonal processes and presynaptic terminals of rodent cerebellum. Brain Res. 85:355–371.
Brandon, C., Lam, D. M. K., andWu, J.-Y. 1979. The γ-aminobutyric acid system in rabbit retina: localization by immunocytochemistry and autoradiography. Proc. Natl. Acad. Sci. U.S.A. 76:3557–3561.
Lam, D. M. K., Su, Y. Y. T., Swain, L., Marc, R. E., Brandon, C., andWu, J.-Y. 1979. Immunocytochemical localization of glutamic acid decarboxylase in goldfish retina. Nature 278:565–567.
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Wu, JY., Welch, K.M.A. & Duncan, C.C. Abnormal protein patterns in multiple sclerosis. Neurochem Res 4, 811–820 (1979). https://doi.org/10.1007/BF00964477
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DOI: https://doi.org/10.1007/BF00964477