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Characteristics of the norepinephrine-stimulated phosphatidylinositol turnover in rat pineal cell dispersions

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Abstract

Dispersed rat pineal cells can be used for the study of the phosphatidylinositol effect. The response to (−)-norepinephrine of the incorporation of32Pi into phospholipids is linear with time and cell concentration, stereospecific, and mediated through α1-adrenergic receptors. Na+ in the incubation medium is obligatory for labeling of phosphatidylinositol and phosphatidylcholine by32P. In the absence of K+ incorporation of32P is drastically lowered and no stimulation by norepinephrine occurs. Rb+ can replace K+. Omission of Ca2+ or substitution with Sr2+ preferentially lowers incorporation of radioactivity into phosphatidylcholine. Mg2+ is not required for basal or stimulated labeling.

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Author notes

  1. Thomas L. Smith

    Present address: Veterans Administration Hospital, 85723, Tucson, Arizona

Authors and Affiliations

  1. Ralph Lowell Laboratories, McLean Hospital, 02178, Belmont, Massachusetts

    George Hauser & Thomas L. Smith

  2. Department of Biological Chemistry, Harvard Medical School, 02115, Boston, Massachusetts

    George Hauser & Thomas L. Smith

Authors
  1. George Hauser
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  2. Thomas L. Smith
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Hauser, G., Smith, T.L. Characteristics of the norepinephrine-stimulated phosphatidylinositol turnover in rat pineal cell dispersions. Neurochem Res 6, 1067–1079 (1981). https://doi.org/10.1007/BF00964413

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