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Interaction of [125I]β nerve growth factor with acidic proteins

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Abstract

We have demonstrated that the β nerve growth factor will interact with various acidic proteins apparently nonspecifically. When125I-labeled β nerve growth factor at a concentration of 3.8×10−10 M is incubated with an acidic protein at 2 mg/ml (4.5×10−6–4.4×10−5 M), a complex is formed. This complex changes the isoelectric point of the125I-labeled β nerve growth factor sufficiently so that the125I-labeled β nerve growth factor migrates anomalously in polyacrylamide gel electrophoresis. The interaction between β nerve growth factor and bovine serum albumin, which appears to be complex, may be the cause of the previously reported activation of the β nerve growth factor when bovine serum albumin is present in a typical bioassay.

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References

  1. Levi-Montalcini, R., andAngeletti, P. 1968. Nerve growth factor. Physiol. Rev. 48:534–569.

    PubMed  Google Scholar 

  2. Server, A. C., andShooter, E. M. 1977. Pages 339–409in Anfinsen, C. B., Edsall, J. T., andRichards, F. M. (eds.), Advances in Protein Chemistry, Academic Press, New York.

    Google Scholar 

  3. Mobley, W. C., Server, A. C., Ishii, D. N., Riopelle, R. J., andShooter, E. M. 1977. Nerve growth factor. N. Engl. J. Med. 297:1096–1104, 1149–1158, 1211–1218.

    PubMed  Google Scholar 

  4. Ronne, H., Anundi, H., Rask, L., andPeterson, P. A. 1979. Nerve growth factor binds to serum alpha-2-monoglobulin. Biochem. Biophys. Res. Commun. 87:330–336.

    PubMed  Google Scholar 

  5. Callisono, P., andCozzari, C. 1974. Interaction of nerve growth factor with the mouse-brain neurotubule protein(s). Proc. Natl. Acad. Sci. U.S.A. 71:2131–2135.

    PubMed  Google Scholar 

  6. Almon, R. R., andVaron, S. 1978. Association of beta nerve growth factor with bovine serum albumin as well as with the alpha and gamma subunits of the 7S macromolecule. J. Neurochem. 30:1559–1567.

    Google Scholar 

  7. Oleander, E. J., andStach, R. W. 1977. High affinity, specific binding of [125I]beta nerve growth factor to glass beads. Biochem. Biophys. Res. Commun. 79:561–568.

    PubMed  Google Scholar 

  8. Stach, R. W., Garian, N., andOleander, E. J. 1979. Biological activity of the β nerve growth factor: The effects of various added proteins. J. Neurochem. 33:257–261.

    PubMed  Google Scholar 

  9. Stach, R. W., Wagner, B. J., andStach, B. M. 1977. A more rapid method for the isolation of the 7S nerve growth factor complex. Anal. Biochem. 83:26–32.

    PubMed  Google Scholar 

  10. Rice, B. L., andStach, R. W. 1976. The dimer-monomer equilibrium constant for125I-β nerve growth factor. Biochem. Biophys. Res. Commun. 73:479–485.

    PubMed  Google Scholar 

  11. Oleander, E. J., andStach, R. W. 1980. Sequestration of125I-labelled β nerve growth factor. J. Biol. Chem. 255:9338–9343.

    PubMed  Google Scholar 

  12. Weber, K., andOsborn, M. 1969. The reliability of molecular weight determinations by dodecylsulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244:4406–4412.

    PubMed  Google Scholar 

  13. Stach, R. W., andShooter, E. M. 1974. The biological activity of cross-linked beta nerve growth factor protein. J. Biol. Chem. 249:6668–6674.

    PubMed  Google Scholar 

  14. Malik, N., andBerrie, A. 1972. A new stain fixative for proteins separated by gel isoelectric focussing based on Coomassie brilliant blue. Anal. Biochem. 49:173–176.

    PubMed  Google Scholar 

  15. Gey, G. O., andGey, M. K. 1936. The maintenance of human normal cells and tumor cells in continuous culture. I. Preliminary report: Cultivation of mesoblastic tumors and normal tissue and notes on methods of cultivation. Am. J. Cancer 27:45–76.

    Google Scholar 

  16. Calissono, P., Monaco, G., Castellani, L., Mercanti, D., andLevi, A. 1978. Nerve growth factor poteintiates actomyosin adenosine triphosphatase. Proc. Natl. Acad. Sci. U.S.A. 75:2210–2214.

    PubMed  Google Scholar 

  17. Pearce, F. L., Banthorpe, D. V., Cook, J. M., andVernon, C. A. 1973. Adsorption of nerve growth factor onto surfaces. Implications for the assay in tissue culture. Eur. J. Biochem. 32:569–575.

    PubMed  Google Scholar 

  18. Greene, L. A., Varon, S., Piltch, A., andShooter, E. M. 1971. Substructure of the β subunit of mouse 7S nerve growth factor. Neurobiology 1:37–48.

    Google Scholar 

  19. Herrup, K., andShooter, E. M. 1973. Properties of the beta nerve growth factor receptor of avian dorsal root ganglia. Proc. Natl. Acad. Sci. U.S.A. 70:3884–3888.

    PubMed  Google Scholar 

  20. Banerjee, S. P., Snyder, S. H., Cuatrecasas, P.,andGreene, L. A. 1973. Binding of the nerve growth factor on synpathetic ganglia. Proc. Natl. Acad. Sci. U.S.A. 70:2519–2523.

    PubMed  Google Scholar 

  21. Banerjee, S. P., Cuatrecasas, P., andSnyder, S. H. 1975. Nerve growth factor receptor binding: Influence of ions, enzymes, and protein reagents. J. Biol. Chem. 250:1417–1433.

    Google Scholar 

  22. Frazier, W. A., Boyd, L. F., andBradshaw, R. A. 1974. Properties of the specific binding of125I-nerve growth factor to responsive peripheral neurons. J. Biol. Chem. 249:5513–5519.

    PubMed  Google Scholar 

  23. Frazier, W. A., Boyd, L. F., Pulliam, M. W., Szutowicz, A., andBradshaw, R. A. 1974. Properties and specificity of binding sites for125I-nerve growth factor in embryonic heart and brain. J. Biol. Chem. 249:5918–5923.

    PubMed  Google Scholar 

  24. Sutter, A., Reopelle, R. J., Harris-Warrick, R. M., andShooter, E. M. 1979. Nerve growth factor receptors. J. Biol. Chem. 254:5972–5982.

    PubMed  Google Scholar 

  25. Malamud, D., andDrysdale, J. W. 1978. Isoelectric points of proteins: a table. Anal. Biochem. 86:620–647.

    PubMed  Google Scholar 

  26. Hedrick, J. L., andSmith, A. J. 1968. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis. Arch. Biochem. Biophys. 126:155–164.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Anatomy State University of New York, Upstate Medical Center, 13210, Syracuse, New York

    Kirt W. Rusenko & Robert W. Stach

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  1. Kirt W. Rusenko
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  2. Robert W. Stach
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Rusenko, K.W., Stach, R.W. Interaction of [125I]β nerve growth factor with acidic proteins. Neurochem Res 6, 287–300 (1981). https://doi.org/10.1007/BF00964044

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