Abstract
The levels of putrescine and spermine in mouse brain were rather constant at different times of day, as were the activities of ornithine andS-adenosyl-l-methionine decarboxylases. Contrary to an earlier report, the level of spermidine was found to be relatively constant. A possibly significant feature in the present results was the steady decline during the light period and rise during darkness of cerebral spermidine and spermine levels, the differences between maximum and minimum being about 15% for both compounds.
Similar content being viewed by others
References
Shaw, G. G. 1979. The polyamines in the central nervous system. Biochem. Pharmac. 28:1–6.
Pajunen, A. E. I., Hietala, O. A., Baruch-Virransalo, E.-L., andPiha, R. S. 1979. The effect ofDl-allylglycine on polyamine and GABA metabolism in mouse brain. J. Neurochem. 32:1401–1408.
Seiler, N., Bink, G., andGrove, J. 1979. Regulatory interrelations between GABA and polyamines. I. Brain GABA levels and polyamine metabolism. Neurochem. Res. 4:425–435.
McCann, P. P., Hornsperger, J.-M., andSeiler, N. 1979. Regulatory interrelations between GABA and polyamines. II. Effect of GABA on ornithine decarboxylase and putrescine levels in cell culture. Neurochem. Res. 4:437–447.
Lapinjoki, S. P., Pajunen, A. E. I., Hietala, O. A., andPiha, R. S. 1980. The effect of polyamines on the enzymes of the 4-aminobutyric acid metabolism in mouse brain in vitro. FEBS Lett. 112:289–292.
Rodichok, L. D., andFriedman, A. H. 1978. Diurnal variations in the toxicity and tissue levels of spermidine in mice. Life Sci. 23:2137–2146.
Seiler, N. 1973. Polyamine metabolism in the brain. Pages 137–156,in Russell, D. H. (ed.), Polyamines in Normal and Neoplastic Growth, Raven Press, New York.
Hölttä, E. 1977. Oxidation of spermidine and spermine in rat liver: Purification and properties of polyamine oxidase. Biochemistry 16:91–100.
Antrup, H., andSeiler, N. 1980. On the turnover of polyamines spermidine and spermine in mouse brain and other organs. Neurochem. Res. 5:123–142.
Hölttä, E., Jänne, J., andHovi, T. 1979. Suppression of the formation of polyamines and macromolecules byDl-α-difluoromethylornithine and methylglyoxal bis(guanylhydrazone) in phytohemagglutinin-activated human lymphocytes. Biochem. J. 178:109–117.
Seiler, N. 1970. Use of the dansyl reaction in biochemical analysis. Methods Biochem. Anal. 18:259–337.
Jänne, J., andWilliams-Ashman, H. G. 1971. On the purification ofl-ornithine decaroxylase from rat prostate and effects of thiol compounds on the enzyme. J. Biol. Chem. 246:1726–1732.
Jänne, J., andWilliams-Ashman, H. G. 1971. Dissociation of putrescine activated decarboxylation ofS-adenosyl-l-methionine from the enzymic synthesis of spermidine and spermine by purified prostatic enzyme preparations. Biochem. Biophys. Res. Commun. 42:222–229.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Cantoni, G. L., andDurrell, J., 1957. Activation of methionine for transmethylation. J. Biol. Chem. 255:1033–1048.
Pegg, A. E., andWilliams-Ashman, H. G. 1969. On the role ofS-adenosyl-l-methionine in the biosynthesis of spermidine by rat prostate. J. Biol. Chem. 244:682–693.
Shaw, G. G. 1979. The synthesis and turnover of spermidine and spermine in mouse brain. Neurochem. Res. 4:269–279.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lapinjoki, S.P., Hietala, O.A., Pajunen, A.E.I. et al. Diurnal levels of polyamines and activities of ornithine andS-adenosyl-l-methionine decarboxylases in mouse brain. Neurochem Res 6, 377–383 (1981). https://doi.org/10.1007/BF00963852
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00963852