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Phosphorylation of the plasma-membrane H+-ATPase of oat roots by a calcium-stimulated protein kinase

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Abstract

When plasma-membrane vesicles isolated from oat (Avena sativa L.) root cells were incubated with [γ-32P]ATP, the H+-ATPase was found to be phosphorylated at serine and threonine residues. Phosphotyrosine was not detected. Endogenous ATPase kinase activity was also observed in plasma-membrane vesicles isolated from potato (Solanum tuberosum L.) root cells as well as from yeast (Saccharomyces cerevisiae). Identity of the phosphorylated oat root Mr=100 000 polypeptide as the ATPase was confirmed using conventional glycerol density-gradient centrifugation to purify the native enzyme and by a new procedure for purifying the denatured polypeptide using reversephase high-performance liquid chromatography. Kinase-mediated phosphorylation of the oat root plasma-membrane H+-ATPase was stimulated by the addition of low concentrations of Ca2+ and by a decrease in pH, from 7.2 to 6.2. These results demonstrate that kinase-mediated phosphorylation of the H+-ATPase is a plausible mechanism for regulating activity. They further indicate that changes in the cytoplasmic [Ca2+] and pH are potentially important elements in modulating the kinase-mediated phosphorylation.

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Abbreviations

EDTA:

ethylenediaminetetraacetic acid

EGTA:

ethylene glycol-bis-(γ-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

Mr :

relative molecular mass

RP-HPLC:

reverse-phase high-performance liquid chromatography

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin, 53706, Madison, WI, USA

    G. Eric Schaller & Michael R. Sussman

  2. Cell/Molecular Biology Program, University of Wisconsin, 53706, Madison, WI, USA

    G. Eric Schaller & Michael R. Sussman

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  1. G. Eric Schaller
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  2. Michael R. Sussman
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Schaller, G.E., Sussman, M.R. Phosphorylation of the plasma-membrane H+-ATPase of oat roots by a calcium-stimulated protein kinase. Planta 173, 509–518 (1988). https://doi.org/10.1007/BF00958964

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  • DOI: https://doi.org/10.1007/BF00958964

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