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Histochemischer Nachweis von Succinodehydrogenase in Biopsien von menschlichem Hirngewebe

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Zusammenfassung

Succinodehydrogenaseaktivität wurde histochemisch in einem Material von 33 Biopsien vom menschlichen Großhirn, Kleinhirn und von Hirntumoren nachgewiesen.

Eine pathologische Verminderung der Enzymaktivität wurde in Cortexpartien in der Nachbarschaft von Hirntumoren gefunden. Zwischen fettiger Degeneration von Nervenzellen und ihrer Enzymaktivität scheint eine inverse Beziehung zu bestehen; Fettanreicherung war stets mit vermindertem Enzymgehalt verbunden. Die Reaktion hyperchromatischer Zellen schien stärker als normal. Verstärkter Enzymgehalt wurde auch in gemästeter Glia des Marklagers gefunden. Sehr schwache Aktivität bestand in Tumorgewebe, jedoch nicht gleich schwach in allen Teilen desselben. Gefäßproliferationen in Glioblastomen waren durch verstärkte Enzymreaktion gekennzeichnet.

Summary

Succinic dehydrogenase was demonstrated histochemically in 33 biopsies of human cerebrum, cerebellum, and from brain tumors.

Enzyme activity was diminished in the cerebral cortex adjacent to brain tumors. An inverse relationship was found between fatty degeneration of neurons and their enzyme content: an increase in fat was consistently accompanied by loss of enzyme. Increased activity was found in hyperchromatic nerve cells and in swollen astrocytes of the edematous white matter. Except for occasional areas with stronger activity, the enzyme activity was found extremely weak in brain tumors. Vascular proliferations in brain tumors exhibited increased enzyme activity.

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Literatur

  • Ahlquist, J., andA. Telkka: Succinic dehydrogenase activity in the kidneys of weanling rats injured by feeding of a choline deficient diet. Acta path. microbiol.41, 235–241 (1957).

    Google Scholar 

  • Baar, H. S.: Succinic dehydrogenase activity of normal and pathological tissues. Schweiz. Z. Path.20, 164–119 (1957).

    Google Scholar 

  • Campbell, A. C. P.: Variation in vascularity and oxidase content in different regions of the brain of the cat. Arch. Neurol. Psychiat. (Chicago)41, 223–242 (1939).

    Google Scholar 

  • Friede, R.: Über Beziehungen zwischen histochemischen Glykogenbefunden und der Hirnwellenfrequenz im EEG an einem Material von menschlichen Biopsien. Arch. Psychiat. Nervenkr.194, 213–237 (1956).

    PubMed  Google Scholar 

  • —: Die histochemische Reifung des Kleinhirnes der Ratte dargestellt durch das Verhalten der Succinodehydrase. Arch. Psychiatr. Nervenkr.196, 196–204 (1957).

    Google Scholar 

  • —: Improved technique for the histochemical demonstration of succinic dehydrogenase in brain tissue. J. Histochem. Cytochem.6, 347–351 (1958).

    PubMed  Google Scholar 

  • - Histochemical investigations on succinic dehydrogenase in the central nervous system. II. Atlas of the medulla oblongata of the guinea pig. J. Neurochem. (im Druck).

  • Kent, S. P.: The effect of post mortem autolysis on certain histochemical reactions. School of Aviation Medicine, Randloph AFB. Tech. Rep. 57-71 (1957).

  • Marinesco, G.: Le role des ferments oxydants dans les phenomena de la vie. Libro en Honor de D. Santiago Ramon y Cajal, Madrid 1922.

  • Mustakallio, K. K.: Histochemical demonstration of succinic dehydrogenase activity in human brain. Ann. Med. exp. Fenn.32, 175–177 (1954).

    PubMed  Google Scholar 

  • Nachlas, M. N., K. C. Tsou, E. Souza, C. S. Cheng andA. M. Seligman: Cytochemical demonstration of succinic dehydrogenase. J. Histochem. Cytochem.5, 420–436 (1957).

    PubMed  Google Scholar 

  • Roizin, L.: Oxidases and peroxidases of the central nervous system. J. Neuropath. exp. Neurol.14, 47–69 (1955).

    PubMed  Google Scholar 

  • Shimizu, N., andN. Morikawa: Histochemical studies of succinic dehydrogenase of the brain of mice, rats, guinea pigs, and rabbits. J. Histochem. Cytochem.5, 334–345 (1957).

    PubMed  Google Scholar 

  • Shimizu, N., N. Morikawa andY. Ishi: Histochemical studies of succinic dehydrogenase and cytochrome oxidase of the rabbit brain, with special reference to the results in the paraventricular structures. J. comp. Neurol.108, 1–14 (1957).

    PubMed  Google Scholar 

  • Wealsch, H., W. M. Sperry andV. A. Stoyanoff: A study of the synthesis and deposition of lipids in brain and other tissues with deuterium as indicator. J. biol. Chem.135, 291–296 (1940).

    Google Scholar 

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Authors and Affiliations

  1. Neurochirurgischen Universitätsklinik Freiburg i. Br., Germany

    Reinhard L. Friede

  2. Aero Medical Laboratories, Wright Air Development Center, Dayton, Ohio

    Reinhard L. Friede

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  1. Reinhard L. Friede
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Friede, R.L. Histochemischer Nachweis von Succinodehydrogenase in Biopsien von menschlichem Hirngewebe. Virchows Arch. path Anat. 332, 216–223 (1959). https://doi.org/10.1007/BF00955932

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  • DOI: https://doi.org/10.1007/BF00955932

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