Abstract
The angiosperm male meiocyte is unusual among plant cells in that during early development ordered cellulosic microfibrils are not deposited at the protoplast surface. Instead, a complex series of events takes place which leads to the formation of the pollen wall ‘primexine’. The use of immuno-cytochemistry, electron microscopy and experiments with an inhibitor have revealed this cell to contain no cortical microtubules, and its cytoskeleton to be radially organised with microtubules extending from the nucleus to the plasma membrane. It is proposed that these microtubules, perhaps organised from the nuclear envelope, play a part in orientation of the nucleus and in the transport of materials to the cell surface.
This is a preview of subscription content, log in via an institution to check access.
References
Bennett, M.D., Smith, J.B., Simpson, S., Wells, B. (1979) Intranuclear fibrillar material in cereal pollen mother cells. Chromosoma71, 289–390
Brown, D.L., Stearns, M.E., MacRae, T.H. (1982) Microtubule organising centres. In: The cytoskeleton in plant growth and development, pp. 55–83, Lloyd, C.W., ed. Academic Press, London New York
De Mey, J., Lambert, A.M., Moreman, M., Debraban, M. (1982) Visualization of microtubules in interphase and mitotic plant cells ofHaemanthus endosperm with immunogold staining method. Proc. Natl. Acad. Sci. USA79, 1898–1902
Dickinson, H.G. (1970) Ultrastructural aspects of primexine formation in the microspore tetrad ofLilium longiflorum. Cytobiologie1, 437–449
Dickinson, H.G. (1971) Nuclear-cytoplasmic interactions following meiosis in the young microspores ofLilium longiflorum; events at the nuclear envelope. Grana11, 117–127
Dickinson, H.G., Heslop Harrison, J. (1971) The mode of growth in the inner layer of the pollen grain exine ofLilium. Cytobios4, 233–243
Fowke, L.C., Bech-Hansen, C.W., Constabel, F., Gamborg, O.L. (1974) A comparative study of the ultrastructure of cultured cells and protoplasts of soybean during cell division. Protoplasma81, 189–203
Gunning, B.E.S., Hardham, A.R., Hughes, J.E. (1978) Evidence for initiation of microtubules in discrete regions of cell cortex inAzolla root-tip cells and an hypothesis on development of cortical arrays of microtubules. Planta143, 161–179
Heslop Harrison, J. (1963) An ultrastructural study of pollen wall ontogeny inSilene pendula. Grana4, 1–24
Heslop Harrison, J. (1971) Sporopollenin in the biological context. In: Sporopollenin, pp. 1–31, Brooks, J.B., Grant, P.R., Muir, M., Van Gijzel, P., Shaw, G. eds. Academic Press, London New York
Jones, J.C.R., Tucker, J.B. (1981) Microtubule-organizing centres and assembly of the double-spiral microtubule pattern in certain heliozoan axonemes. J. Cell Sci.50, 259–280
Kilmartin, J.V., Wright, B., Milstein, C. (1982) Rat monoclonal antitubulin antibodies derived by using a new non-secreting rat-cell line. J. Cell Biol.93, 576–582
Lloyd, c.W., Barlow, P.W. (1982) The co-ordination of cell division and elongation: the rôle of the cytoskeleton. In: The cytoskeleton in plant growth and development, pp. 203–228, Lloyd, C.W., ed.. Academic Press, London New York
Lloyd, C.W., Slabas, A.R., Powell, A.J., Peace, G.W. (1982) Novel features of the plant cytoskeleton. Cell Biol. Int. Rep.6, 171–175
Mazia, D., Schatten, G., Sale, W. (1975) Adhesion of cells to surfaces coated with polylysine. J. Cell Biol.66, 198–200
Mattsson, O. (1976) The development of dimorphic pollen inTripogandra (Commelinaceae). In: Linn. Soc. Symp. Ser. 1: The evolutionary significance of the exine, pp. 163–183, ferguson, I.K., Muller, J., eds. Academic Press, London New York
Powell, A.J., Peace, J.W., Slabas, A.R., Lloyd, C.W. (1982) The detergent-resistant cytoskeleton of higher-plants protoplasts contains nucleus associated fibrillar bundles in addition to microtubules. J. Cell Sci.56, 319–335
Rasmussen, S.W., Holm, P.B. (1980) Mechanics of meiosis. Hereditas93, 187–216
Sheldon, J.M., Dickinson, H.G. (1983) Determination of patterning in the pollen wall ofLilium henryi. J. Cell Sci.63, 191–208
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dickinson, H.G., Sheldon, J.M. A radial system of microtubules extending between the nuclear envelope and the plasma membrane during early male haplophase in flowering plants. Planta 161, 86–90 (1984). https://doi.org/10.1007/BF00951464
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00951464