Skip to main content
SpringerLink
Log in

Diagnosis of intraocular lymphoma by polymerase chain reaction

  • Clinical Investigation
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

• Background: Cytopathological examinations have been used in the diagnosis of intraocular lymphoma. However, sometimes it is not easy to detect malignant cells in the biopsy specimens. We applied a method that identified monoclonal proliferation of B lymphocytes by using polymerase chain reaction (PCR) in the diagnosis of patients suspected to have intraocular B-cell lymphoma. • Methods: Threè specimens of the diagnostic vitrectomy were studied by cytological examination and by PCR to amplify the complementary determining region (CDR3) of immunoglobulin heavy chain (IgH) gene. As a positive control, a biopsy specimen of an orbital lymphoma was examined; four vitrectomy specimens from patients with diabetic retinopathy, proliferative vitreo-retinopathy, acute retinal necrosis (ARN) and macular hole were negative controls. • Results: On cytologic examination, no malignant cells were found in three specimens: suspected intraocular lymphoma and one ARN. In contrast, a discrete band that reflected monoclonal proliferation of B lymphocytes was detected by PCR in specimens from two patients and the positive control. Vitrectomy specimens from the negative controls, including ARN, did not show a discrete band on PCR. • Conclusions: Two cases of ocular malignant lymphoma were diagnosed by PCR identification of monoclonal proliferation of B lymphocytes. This method may be an additional diagnostic tool in the diagnosis of intraocular B-cell lymphoma.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brisco M, Tan L, Orsborn A, Morley A (1990) Development of a highly sensitive assay, based on the polymerase chain reaction, for rare Blymphocyte clones in a polyclonal population. Br J Haematol 75: 163–167

    PubMed  Google Scholar 

  2. Blumenkranz MS, Ward T, Murphy S, Mieler W, Williams GA, Long J (1992) Applications and limitations of vitreoretinal biopsy techniques in intraocular large cell lymphoma. Retina 12:S64-S70

    PubMed  Google Scholar 

  3. Char DH, Ljung B, Deschenes J, Miller T (1988) Intraocular lymphoma: immunological and cytological analysis. Br J Ophthalmol 72:905–911

    PubMed  Google Scholar 

  4. Davis JL, Solomon D, Nussenblatt RB, Palestine AG, Chan C (1992) Immunocytochemical staining of vitreous cells. Ophthalmology 99:250–256

    PubMed  Google Scholar 

  5. de Smet MD, Nussenblatt RB, Davis JL, Palestine AG (1990) Large cell lymphoma masquerading as a viral retinitis.Int Ophthalmol 14:413–417

    PubMed  Google Scholar 

  6. Freeman L, Schachat A, Knox D, Michels R, Green W (1987) Clinical features, laboratory investigations, and survival in ocular reticulum cell sarcoma. Ophthalmology 94: 1631–1639

    PubMed  Google Scholar 

  7. Goldey SH, Stern GA, Oblon DJ, Mendenhall NP, Smith LJ, Duque RE (1989) Immunophenotypic characterization of an unusual T-cell lymphoma presenting as anterior uveitis. A clinicopathologic case report. Arch Ophthalmol 107:1349–1353

    PubMed  Google Scholar 

  8. Jakobiec FA, Neri A, Knowles II DM (1987) Genotypic monoclonality in immunophenotypically polyclonal orbital lymphoid tumors. A mode1 of tumor progression in the lymphoid system. The 1986 Wendell Hughes lecture. Ophthalmology 94:980–994

    PubMed  Google Scholar 

  9. Kyle RA (1994) Monoclonal gammopathy of undetermined significance. Blood Rev 8:135–141

    PubMed  Google Scholar 

  10. Maniatis T, Fritsh EF, Sambrook J (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York, pp 9.16–9.19

    Google Scholar 

  11. Matzkin DC, Slamovits TL, Rosenbaum PS (1994) Simultaneous Intraocular and orbital non-Hodgkin lymphoma in the acquired immune deficiency syndrome. Ophthalmology 101:850–855

    PubMed  Google Scholar 

  12. Neri A, Jakobiec FA; Pelicci PG; Dalla-Favera R, Knowles D (1987) Immunoglobulin and T cell receptor beta chain gene rearrangement analysis of ocular adnexal lymphoid neoplasms: clinical and biologie implications. Blood 70:1519–1529

    PubMed  Google Scholar 

  13. Non-Hodgkin's Lymphoma Pathologie Classification Project (1982) National Cancer Institute sponsored study of classifications of non-Hodgkin's lymphomas: summary and description of a working formulation for clinical usage. Cancer 49:2112–2135

    Google Scholar 

  14. Pavan PR, Oteiza EE, Margo CE (1995) Oeular lymphoma diagnosed by internal subretinal pigment epithelium biopsy. Areh Ophthalmol 113:1233–1234

    Google Scholar 

  15. Peterson K, Gordon KB, Heinemann MH, DeAngelis LM (1993) The clinical spectrum of oeular lymphoma. Cancer 72:843–849

    PubMed  Google Scholar 

  16. Potter MN, Cross NC, van Dongen JJ, Saglio G, Oakhill A, Bartram CR, Goldman JM (1993) Molecular evidence of minimal residual disease after treatment for leukaemia and lymphoma: an updated meeting report and review. Leukemia 7:1302–1314

    PubMed  Google Scholar 

  17. Ramasamy I, Brisco M, Morley A (1992) Improved PCR method for detecting monoclonal immunoglobulin heavy chain rearrangement in B cell neoplasms. J Clin Pathol 45:770–775

    PubMed  Google Scholar 

  18. Ridley M, McDonald HP, Sternberg J, Blumenkranz M, Zarbin M, Sehachat A (1992) Retinal manifestations of ocular lymphoma (reticulum cell sacroma). Ophthalmology 99:1153–1161

    PubMed  Google Scholar 

  19. Schatz DG, Oettinger MA, Sehlissel MS (1992 V(D)J recombination: molecular biology and regulation. Annu Rev Immunol 10: 359–383 11.11

    PubMed  Google Scholar 

  20. Tonegawa S (1983) Somatic generation of antibody diversity. Nature 302:575–581

    Google Scholar 

  21. Trainor KJ, Brisco MJ, Story CJ, Moriey AA (1990) Monoelonarity in B-lymphoproliferative disorders detected at the DNA level. Blood 75:2220–2222

    PubMed  Google Scholar 

  22. Whitcup SM, de Smet MD, Rubin BI, Palestine AG, Martin DF, Burnier M Jr, et al. (1993) Intraocular lymphoma. Clinical and histopathologic diagnosis. Ophthalmology 100:1399–1406

    PubMed  Google Scholar 

  23. Wilson DJ, Braziel R, Rosenbaum JT (1992) Intraocular lymphoma. Immunopathologic analysis of vitreous biopsy specimens. Arch Ophthalmol 110:1455–1458

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Shinshu University School of Medicine, 3-1-1 Asahi, 390, Matsumoto, Japan

    Naomichi Katai, Sachiko Kuroiwa & Nagahisa Yoshimura

  2. Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan

    Kazuya Fujimori

Authors
  1. Naomichi Katai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sachiko Kuroiwa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazuya Fujimori
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nagahisa Yoshimura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Naomichi Katai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Katai, N., Kuroiwa, S., Fujimori, K. et al. Diagnosis of intraocular lymphoma by polymerase chain reaction. Graefe's Arch Clin Exp Ophthalmol 235, 431–436 (1997). https://doi.org/10.1007/BF00947062

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00947062

Keywords

Search

Navigation