Abstract
In vitro agglutinability of trophozoites of threeEntamoeba histolytica strains, cultivated under axenic conditions in the presence of concanavalin A (Con A), was shown to be related to the degree of their pathogenicity for experimental animals and of the concentration of Con A. Seven strains ofE. invadens tested also agglutinated in the presence of Con A, and the degree of agglutination was proportional to the concentration of the lectin. Three strains ofE. histolytica-like “Laredo-type” amebae, a strain ofE. terrapinae, and a strain ofE. moshkovskii agglutinated very slightly, only in the presence of the highest concentration of Con A tested.
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References
Barker DC (1965) The incidence ofEntamoeba invadens in a natural population of reptiles in southwest Madagscar Progress in Protozoology, Excerpta Medica International Congress Series No. 91, p. 195
Beaver PC, Jung RC, Sherman HJ, Read TR, Robinson TA (1956) ExperimentalEntamoeba histolytica infections in man. Am J Trop Med Hyg 5:1000–1009
Bos HJ, Hage AJ (1975) Virulence of bacteria-associated,Crithidia-associated, and axenicEntamoeba histolytica: experimental hamster liver infections with strains from patients and carriers. Z Parasitenkd 47:79–89
Bos HJ, van de Griend RJ (1977) Virulence and toxicity of axenicEntamoeba histolytica. Nature 265:341–343
Das SR (1977) Concanavalin A-induced agglutination in pathogenic and non-pathogenic anaerobic and aerobic amoebae. Int J Parasitol 1:5–7
Deschiens R (1937) Considerations sur l'action pathogène d'une souche d'amibes dysenteriques (Souche: C Dobell D.K.B.) Bull Soc Pathol Exot Filiales 30:562–564
Diamond LS (1960) The exenic cultivation of two reptilian parasites,Entamoeba terrapinae Sanders & Cleveland, 1930, andE. invadens Rodhain 1934. J Parasitol 46:484
Diamond LS (1968) Techniques of axenic cultivation ofEntamoeba histolytica Shaudinn, 1903 andE. histolytica-like amoebae. J Parasitol 54:1047–1056
Diamond LS, Harlow DR, Cunnick CC (1978) A new medium for the axenic cultivation ofEntamoeba histolytica and otherEntamoeba. Trans R Soc Trop Med Hyg 72:431–432
Dreyer DA (1961) Growth of a strain ofEntamoeba histolytica at room temperature. Texas Rep Biol Med 19:393–396
Entner N, Most H (1965) Genetics ofEntamoeba: characterization of two new parasitic strains which grow at room temperature (and at 37°C). J Protozool 12:10–13
Ghadirian E, Meerovitch E (1978) Behavior of axenic IP-106 strain ofEntamoeba histolytica in the golden hamster. Am J Trop Med Hyg 27:241–247
Ghardirian E, Meerovitch E (1979) Pathogenicity of axenically cultivatedEntamoeba histolytica, strain 200:NIH, in the hamster. J Parasitol 65:768–771
Gray CW, Marcus LC, McCarten WC, Sappington T (1966) Amebiasis in the Komodo Dragon,Varanus komodoensis. Int Zoo Year Book 6:279–283
Lachance PJ (1959) A Canadian strain ofEntamoeba moshkovskii Chalaia, 1941 Can J Zool 37:415–417
Martinez-Palomo A, Gonzalez-Robles A, de la Torre M (1973) Selective agglutination of pathogenic strains ofEntamoeba histolytica induced by Con A. Nature (New Biology) 245:186–187
McConnachie E (1958) Factors affecting the growth and behaviour of axenic strains ofEntamoeba invadens Rodhain, 1934. Parasitology 48:423–432
Meerovitch E (1958) Some biological requirements and host-parasite relations ofEntamoeba invadens. Can J Zool 36:513–523
Meerovitch E, Eaton RDP, McLaughlin J (1976) Functional aspects of host-parasite relations in amoebiasis. Proceedings of the International Conference on Amebiasis, Mexico City, October 1975, Instituto Mexicano del Seguro Social, pp 628–635
Mizgireva MF (1966) Comparative vurulence ofEntamoeba histolytica strains isolated from patients and different groups of carriers. Med Parazitol Parazitar Bolez (Moscow) 35:201–204
Phillips BP, Wolfe PA, Bartgis IL (1958) Studies on the ameba-bacteria relationship in amebiasis. II Some concepts in the etiology of the disease. Am J Trop Med Hyg 7:392–399
Ross GW, Knight R (1973) Dietary factors affecting the pathogenicity ofEntamoeba histolytica in rats. Trans R Soc Trop Med Hyg 67:560–567
Steck F (1963) Die Ameobendysenterie der Reptilien. Acta Trop (Berl), 20:115–142
Trissl D, Martinez-Palomo, Arguello C, de la Torre M, de la Hoz R (1977) Surface properties related to Concanavalin A-induced agglutination. A comparative study of severalEntamoeba strains. J Exp Med 145:652–665
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Ghadirian, E., Meerovitch, E. Lectin-induced agglutination of trophozoites of different species and strains ofEntamoeba . Z. Parasitenkd. 70, 147–152 (1984). https://doi.org/10.1007/BF00942216
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DOI: https://doi.org/10.1007/BF00942216