Summary
Cephalosporium acremonium CW 19 produces cephalosporin C from sulfate; production is increased by addition of methionine, a source of sulfur for the antibiotic. Mutation to methionine auxotrophy eliminates the ability to produce antibiotic at low methionine concentration (30–80 μg DL-methionine per ml) despite normal growth and the presence of excess sulfate for antibiotic synthesis. Thus cephalosporin C biosynthesis from sulfate by the parent culture involves some obligatory role of endogenous methionine, probably a non-precursor role.
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Drew, S.W., Demain, A.L. The obligatory role of methionine in the conversion of sulfate to cephalosporin C. European J. Appl Microbiol. 1, 121–128 (1975). https://doi.org/10.1007/BF00942209
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DOI: https://doi.org/10.1007/BF00942209