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A taxonomic analysis of seed proteins inPinus spp.(Pinaceae)

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Abstract

Seed storage proteins have proved to be a powerful biochemical marker for taxonomic research, but they have not been extensively employed in forest tree studies. In order to improve the understanding of the taxonomy of the genusPinus, total seed proteins of 12 pine species have been analyzed by means of SDS-PAGE (Sodium dodecylsulphate-polyacrylamide gel electrophoresis). The results showed the presence, in the genusPinus, of two main sub-taxa, corresponding to the subgeneraHaploxylon andDiploxylon. Differences and affinities between Mediterranean pine species were found in agreement with classification ofKlaus (1989).

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References

  • Aliaga-Morell, J. R., Culianez-Macia, F. A., Clemente-Marin, G., Primo-Yufera, E., 1987: Differentiation of rice varieties by electrophoresis of embryo protein. — Theor. Appl. Genet.74: 224–232.

    Google Scholar 

  • Burges, S. R., Shewry, P. R., 1986: Identification of homologous globulins from embryos of wheat, barley, rye and oats. — J. Exp. Bot.37: 1863–1871.

    Google Scholar 

  • Collada, C., Caballero, R. G., Casado, R., Aragoncillo, C., 1988: Different types of major storage seed proteins inFagaceae species. — J. Exp. Bot.39: 1751–1758.

    Google Scholar 

  • Conkle, M. T., Schiller, G., Grunwald, C., 1988: Electrophoretic analysis of diversity and phylogeny ofPinus brutia and closely related taxa. — Syst. Bot.13: 411–424.

    Google Scholar 

  • Critchfield, W. B., Little, E. L., 1966: Geographic distribution of the pines of the world. — US. Dept. Agr. Misc. Pub. 991. — Washington D. C.

  • De Dominicis, R. I., Vosa, C. G., 1989: Il nucleolo nelle gimnosperme. Dati preliminari in 10 specie di Pinus. — Giorn. Bot. Ital.123, Suppl. 1: 121.

    Google Scholar 

  • Duffield, J. W., 1953: Relationship and species hybridization in the genusPinus. — Z. Forstgenetik1: 93–97.

    Google Scholar 

  • Durzan, D. J., 1966: Disc electrophoresis of soluble protein in the female gametophyte and embryo of conifer seed. — Canad. J. Bot.44: 359–360.

    Google Scholar 

  • Ehrendorfer, F., 1976: Evolutionary significance of chromosomal differential patterns in gymnosperms and primitive angiosperms. — InBeck, C. B., (Ed.): Origin and early evolution of angiosperms, pp. 220–240. — New York: Columbia University Press.

    Google Scholar 

  • Farjon, A., 1984: Pines: drawings and descriptions of the genus. — Leiden: E. J. Brill.

    Google Scholar 

  • Florence, L. Z., Hicks, R. R., 1980: Further evidence for introgression ofPinus taeda withP. echinata: electrophoretic variability and variation in resistance toCronartium fusiforme. — Silvae Genet.29: 41–43.

    Google Scholar 

  • Francini-Corti, E., 1961: Appunti evolutivi sui generiPinus eQuercus desunti dal ciclo riproduttivo. — Acc. Naz. Linc. Quaderno47: 93–105.

    Google Scholar 

  • Gaussen, H., 1960: Les gymnospermes actuelles et fossiles. — Trav. Lab. Forest. Toulouse2: 1. sect. 6 GenrePinus. — Toulouse.

    Google Scholar 

  • Gifford, D. J., 1988: An electrophoretic analysis of the seed proteins fromPinus monticola and eight other species of pine. — Canad. J. Bot.66: 1808–1812.

    Google Scholar 

  • —, 1989: Lodgepole pine seed germination. I. Changes in peptidase activity in the megagametophyte and embryonic axis. — Canad. J. Bot.67: 2539–2543.

    Google Scholar 

  • Hagman, M., 1967: Serological studies of pollen and the incompatibility in forest trees. — IUFRO Kongress, München 1967. Papers vol. III, 60–71.

    Google Scholar 

  • —, 1977: The use of serological methods for the identification of species, provenances and clones of forest trees. — EEC Symp. on Forest Trees Biochemistry, Brussels: 227–263.

    Google Scholar 

  • Jensen, U., Berthold, H., 1989: Legumin-like proteins in gymnosperms. — Phytochemistry28: 1389–1394.

    Google Scholar 

  • Khoshoo, T. N., 1962: Cytogenetical evolution in gymnosperms-karyotype. — Proc. Summer School, Darjeeling, Gort of India 1962: 119–135.

    Google Scholar 

  • King, G. J., 1986: A taxometric analysis of seed proteins in the genusBriza s.l. (Poaceae). — Pl. Syst. Evol.151: 163–174.

    Google Scholar 

  • Klaus, W., 1989: Mediterranean pines and their history. — Pl. Syst. Evol.162: 133–163.

    Google Scholar 

  • Konarev, V. G., Gavriljuk, I. P., Gubareva, N. K., 1987: Storage proteins in the identification of species, cultivars and lines. — Seed Sci. Technol.15: 675–678.

    Google Scholar 

  • Ladizinsky, G., Hymowitz, T., 1979: Seed protein electrophoresis in taxonomic and evolutionary studies — review. — Theor. Appl. Genet.54: 145–151.

    Google Scholar 

  • Laemmli, U. K., 1970: Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. — Nature227: 680–685.

    PubMed  Google Scholar 

  • Lafiandra, D., Benedetelli, D., Margiotta, B., Spagnoletti-Zeuli, P. L., Porceddu, E., 1990: Seed storage proteins and wheat genetic resources. — InSrivastava, J. P., Damania, A. B., (Eds.): Wheat genetic resources: meeting diverse needs, pp. 73–87. — Aleppo, Syria: ICARDA.

    Google Scholar 

  • Lalonde, L. D., Fountain, D. W., Kermode, A., Ouellette, F. B., Scott, K., Bewley, J. D., Gifford, D. J., 1984: A comparative study of the insoluble storage proteins and the lectins of seed of theEuphorbiaceae. — Canad. J. Bot.62: 1671–1677.

    Google Scholar 

  • Levin, D. A., Wilson, A. C., 1976: Rates of evolution in seed plants: net increase in diversity of chromosome numbers and species numbers through time. — Proc. Natl. Acad. Sci. U.S.A.73: 2086–2090.

    Google Scholar 

  • Miege, M. N., 1982: Protein types and distribution. — InBoulter, C., Parthier, B., (Eds.): Nucleic acids and proteins in plant, pp. 291–315. — Berlin: Springer.

    Google Scholar 

  • Mirov, N. T., 1967: The genusPinus. — New York: Ronald Press.

    Google Scholar 

  • Ohri, D., Khoshoo, T. N., 1986: Genome size in gymnosperms. — Pl. Syst. Evol.153: 119–132.

    Google Scholar 

  • Payne, P. I., Corfield, K. G., Holt, L. M., Blackman, J. A., 1981: Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. — J. Sci. Food Agric.32: 51–60.

    Google Scholar 

  • Pederick, L. A., 1970: Chromosome relationships between pine species. — Silvae Genet.19: 171–180.

    Google Scholar 

  • Pei-Show, J., Stotzky, G., 1970: Electrophoretic patterns of globulins and albumins in conifer seeds. — Canad. J. Bot.48: 1911–1912.

    Google Scholar 

  • —, —, 1973: Electrophoretic analysis of isozymes from seeds ofPinus, Abies andPseudotsuga. — Canad. J. Bot.51: 2201–2205.

    Google Scholar 

  • Pilger, R., 1926: GenusPinus. — InEngler, A., Prantl, K., (Eds.): Die natürlichen Pflanzenfamilien, 13,Gymnospermae. 2nd edn. — Leipzig: Engelmann.

    Google Scholar 

  • Prager, E. M., Fowler, D. P., Wilson, C. A., 1976: Rates of evolution in conifers (Pinaceae). — Evolution30: 637–649.

    Google Scholar 

  • Price, R. A., 1989: The genera ofPinaceae in the southeastern United States. — J. Arnold Arbor.70: 247–305.

    Google Scholar 

  • Prus-Glowacki, W., Szweykowski, J., 1983: Studies on isozyme variability in populations ofPinus sylvestris L.,P. mugo Turra,P. uliginosa Neuman and individuals from a hybrid swarm population. — Bull. Soc. Amis Sci. Lett. Poznan Ser. D22: 107–122.

    Google Scholar 

  • —, —,Nowak, R., 1985: Serotaxonomical investigation of the european pine species. — Silvae Genet.34: 162–170.

    Google Scholar 

  • Saito, A., 1968: The phytoserological study on the phylogenic relationship inPinus. — Bull. Kyushu Univ. Forests42: 235–240.

    Google Scholar 

  • Sax, K., Sax, H. J., 1933: Chromosome number and morphology in the conifers. — J. Arnold Arbor.14: 356–375.

    Google Scholar 

  • Saylor, L. C., 1964: Karyotype analysis ofPinus-groupLariciones. — Silvae Genet.13: 165–170.

    Google Scholar 

  • —, 1972: Karyotype analysis of the genusPinus-subgenusPinus. — Silvae Genet.21: 155–163.

    Google Scholar 

  • —, 1983: Karyotype analysis of the genusPinus-subgenusStrobus. — Silvae Genet.32: 119–126.

    Google Scholar 

  • Shaw, G. R., 1914: The genusPinus. — Publ. Arnold Arbor.1: 1–96.

    Google Scholar 

  • Schiller, G., Conkle, M. T., Grundwald, C., 1986: Local differentiation among Mediterranean populations of Aleppo pine in their isoenzymes. — Silvae Genet.35: 11–19.

    Google Scholar 

  • Stegemann, H., 1983: Discrimination among infraspecific taxa using electrophoretic data. — InJensen, U., Fairbrothers, D. E., (Eds.): Proteins and nucleic acids in plant systematics, pp. 124–128. — Berlin, Heidelberg, New York, Tokyo: Springer.

    Google Scholar 

  • Strauss, S. H., Doerksen, A. H., 1990: Restriction fragment analysis of pine phylogeny. — Evolution44: 1081–1096.

    Google Scholar 

  • Szmidt, A. E., Sigurgeirsson, A., Wang, X.-R., Hallgren, J.-K., Lindgren, D., 1988: Genetic relationships amongPinus species based on chloroplast DNA polymorphism. — Proceedings of the Frans Kempe Symposium Molecular Genetics of Forest Trees June 14–16TH Umeå, Sweden: J.-E. Hallgren.

    Google Scholar 

  • Thielges, B. A., 1969: A chromatographic investigation of interspecific relationship inPinus (subsectionSylvestris). — Amer. J. Bot.56: 406–409.

    Google Scholar 

  • Vaughan, J. G., 1983: The use of seed proteins in taxonomy and phylogeny. — InDaussant, J., Mosse, J., Vaughan, J., (Eds.): Seed proteins, pp. 135–150. — London, New York: Academic Press.

    Google Scholar 

  • Wilkinson, L., 1985: Systat: User's manual. — Systat Inc. Evanston.

    Google Scholar 

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Schirone, B., Piovesan, G., Bellarosa, R. et al. A taxonomic analysis of seed proteins inPinus spp.(Pinaceae) . Pl Syst Evol 178, 43–53 (1991). https://doi.org/10.1007/BF00937981

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  • DOI: https://doi.org/10.1007/BF00937981

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