Abstract
Restriction endonuclease fragment analysis of nuclear ribosomal DNA (rDNA) was completed on 25 individuals each from seven populations of theLisianthius skinneri (Gentianaceae) species complex in Panama. Seven restriction enzymes were used to determine the amount and type of rDNA variation within and among individuals of the populations. No restriction site variation was seen within populations or individuals although site differences were seen among populations. Spacer length variation within and among individuals of populations was mapped to the internal transcribed spacer (ITS) region between the 18S and 5.8S rRNA genes, a region inLisianthius rDNA that previously was shown to exhibit length differences among populations. This is the first reported case of such variation within and among individuals of populations for the ITS region. Presence or absence of ITS spacer length variation is not correlated with levels of isozymic heterozygosity within populations. No detectable length variation within individuals or populations was seen in the larger intergenic spacer (IGS). Although populations varied with respect to IGS length, all individuals of a given population had a single and equivalent IGS length.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Appels, R., Dvorak, J., 1982: The wheat ribosomal spacer region: its structure and variation in populations and among species. — Theor. Appl. Genet.63: 337–348.
—,Honeycutt, R. L., 1986: rDNA: evolution over a billion years. — InDutta, S. K., (Ed.): DNA systematics 2: plants, pp. 81–135. — Boca Raton, Florida: CRC Press.
Arnheim, N., 1983: Concerted evolution of multigene families. — InNei, M., Koehn, R. K., (Eds.): Evolution of genes and proteins, pp. 38–61. — Sunderland, Mass.: Sinauer.
Cluster, P. D., Jorgensen, R. A., Bernatsky, R., Hakim-Elahi, A., Allard, R. W., 1984: The genetics and geographical distribution of ribosomal DNA spacer-length variation in the wild oat,Avena barbata. — Genetics107: s21.
Coen, E. S., Strachan, T., Dover, G., 1982a: The dynamics of concerted evolution in the ribosome and histone gene families in theDrosophila melanogaster species subgroup. — J. Mol. Biol.158: 17–25.
—,Thoday, E. S., Dover, G., 1982b: Rate of turnover of structural variants in rDNA gene family ofDrosophila melanogaster. — Nature295: 564–568.
Dover, G., 1982: Molecular drive: a cohesive mode of species evolution. — Nature299: 111–117.
—,Brown, S., Coen, E., Dallas, J., Strachan, T., Trick, M., 1982: The dynamics of genome evolution and species differentiation. — InDover, G. A., Flavell, R. B., (Eds.): Genome evolution, pp. 342–372. — New York: Academic Press.
Doyle, J. J., Beachy, R. N., 1985: Ribosomal gene variation in soybean (Glycine) and its relatives. — Theor. Appl. Genet.70: 369–376.
Erdmann, V. A., 1982: Collection of published 5S and 5.8S RNA sequences and their precursors. — Nucleic Acids Res.10: r93–r115.
Flavell, R. B., O'Dell, M., Sharp, P., Nevo, E., Beiles, A., 1986: Variation in the intergenic spacer of ribosomal DNA of wild wheat,Triticum dicoccoides, in Israel. — Mol. Biol. Evol.3: 547–558.
Hamrick, J. L., Linhart, Y. B., Mitton, J. B., 1979: Relationships between life history characteristics and electrophoretically detectable genetic variation in plants. — Ann. Rev. Ecol. Syst.10: 173–200.
Lamppa, G. K., Honda, S., Bendich, A. J., 1984: The relationship between ribosomal repeat length and genome size inVicia. — Chromosoma89: 1–7.
Learn, G. H., Schaal, B. A., 1987: Population subdivision for ribosomal DNA repeat variants inClematis fremontii. — Evolution41: 433–438.
Loveless, M. D., Hamrick, J. L., 1984: Ecological determinants of genetic structure in plant populations. — Ann. Rev. Ecol. Syst.15: 65–95.
Nevo, E., Beiles, A., 1988: Ribosomal DNA non-transcribed spacer polymorphism in subterranean mole rats: genetic differentiation, environmental correlates and phylogenetic relationships. — Evol. Ecol.2: 139–156.
Rafalski, J. A., Wievíoroswki, M., Söll, D., 1983: Organization of ribosomal DNA in yellow lupine (Lupinus luteus) and sequence of the 5.8S RNA gene. — Fed. Eur. Biochem. Soc. Lett.152: 241–246.
Rogers, S. O., Honda, S., Bendich., A. J., 1986: Variation in the ribosomal RNA genes among individuals ofVicia faba. — Pl. Mol. Biol.6: 339–345.
Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A., Allard, R. W., 1984: Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and — population dynamics. — Proc. Natl. Acad. Sci. USA81: 8014–8018.
Schaal, B. A., 1985: Genetic variation in plant populations: from demography to DNA. — InHaeck, J., Woldendorp, J., (Eds.): Structure and functioning of plant populations, pp. 321–324. — Amsterdam: North-Holland.
—,Leverich, W. J., Nieto-Sotelo, J., 1987: Ribosomal DNA variation in the native plantPhlox divaricata. — Mol. Biol. Evol.4: 611–621.
Smith, G. P., 1975: Evolution of repeated DNA sequences by unequal crossover. — Science191: 528–535.
Sytsma, K. J., 1988: Taxonomic revision of the Central AmericanLisianthius skinneri species complex (Gentianaceae). — Ann. Missouri Bot. Gard.75: 1587–1602.
—,Schaal, B. A., 1985a: Genetic variation, differentiation, and evolution in a species complex of tropical shrubs based on isozymic data. — Evolution39: 582–593.
—, —, 1985b: Phylogenetics of theLisianthius skinneri (Gentianaceae) complex in Panama utilizing DNA restriction fragment analysis. — Evolution39: 594–608.
Walbot, V., Cullis, C. A., 1985: Rapid genomic change in higher plants. — Ann. Rev. Pl. Physiol.36: 367–396.
Weaver, R. E., Jr., 1972: A revision of the neotropical genusLisianthius (Gentianaceae). — J. Arnold Arbor.53: 76–100, 234–311.
Williams, S. M., DeSalle, R., Strobeck, C., 1985: Homogenization of geographical variants at the nontranscribed spacer of rDNA inDrosophila mercatorum. — Mol. Biol. Evol.2: 338–347.
Yakura, K., Kato, A., Tanifuji, S., 1984: Length heterogeneity in the large spacer ofVicia faba rDNA is due to the differing number of a 325 bp repetitive sequence elements. — Molec. Gen. Genet.193: 400–405.
Zimmer, E. A., Martin, S., Beverley, S., Kan, Y. W., Wilson, A. C., 1980: Rapid duplications and loss of the genes coding for the chains of hemoglobin. — Proc. Natl. Acad. Sci. USA77: 2158–2162.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sytsma, K.J., Schaal, B.A. Ribosomal DNA variation within and among individuals ofLisianthius (Gentianaceae) populations. Pl Syst Evol 170, 97–106 (1990). https://doi.org/10.1007/BF00937852
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00937852