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Cold-sensitive chromosome regions and heterochromatin inCestrum aurantiacum (Solanaceae)

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Abstract

The karyotype ofCestrum aurantiacum was analyzed for the presence of coldsensitive regions (CSRs) and other types of constitutive heterochromatin. A range of techniques was employed including the fluorescent DAPI, chromomycin/DAPI double staining and actinomycin D/DAPI counter-staining, and the non-fluorescent C-banding applied as single or sequential staining, sequential N-banding and silver impregnation. Four classes of constitutive heterochromatin were recognized: CSRs, nucleolar organizers, non-nucleolar chromomycin-positive bands, and indifferently fluorescent bands. The banded karyotype ofC. aurantiacum is compared with those of otherCestrum species. The sectionsHabrothamnus andCestrum are not karyologically distinct.

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Berg, C., Greilhuber, J. Cold-sensitive chromosome regions and heterochromatin inCestrum aurantiacum (Solanaceae). Pl Syst Evol 185, 259–273 (1993). https://doi.org/10.1007/BF00937662

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