Abstract
Trypanosomes, isolated from the gut of a naturally infectedGlossina pallidipes in Kiboko, Kenya, were grown in vitro. The cultured trypanosomes (“F4”-stock) showed a wide variety in morphological stages, not characteristic of the salivarian trypanosomes that are known to occur in the Kiboko area. Identification of the “F4”-stock was attempted by isoenzyme studies, infection of tsetse flies and of experimental animals. Electrophoretic isoenzyme patterns of the “F4”-organisms were developed for ten soluble enzymes and compared with those of 5 reference stocks of salivarian trypanosomes and 2 stocks ofTrypanosoma theileri. For theT. theileri comparisons six enzymes were used. It was found that the “F4”-trypanosome clearly differed from the 3 salivarian species and fromT. theileri. Experimental infection of laboratory bredGlossina m. morsitans showed that the “F4”-organisms developed in the posterior station of the alimentary tract and the cultured trypanosomes were infective to non-indigenous reptiles.
This is believed to be the first record of the isolation and the isoenzyme characterisation of a non-salivarian reptile trypanosome from a naturally infected tsetse fly of themorsitans group (subgenusGlossina) in a savannah area in Africa.
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Minter-Goedbloed, E., Pudney, M., Kilgour, V. et al. First record of a reptile trypanosome isolated fromGlossina pallidipes in Kenya. Z. Parasitenkd. 69, 17–26 (1983). https://doi.org/10.1007/BF00934006
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DOI: https://doi.org/10.1007/BF00934006