Abstract
The relationship between the hookwormAncylostoma ceylanicum and the host intestinal mucosa in situ has been examined by using an in vivo fixation technique and scanning electron microscopy. Heads of the adult worms were buried deeply in the mucosa of the dog's small bowel. At some sites of attachment there was severe atrophy and ulceration of the immediately surrounding villi. Superficial deposits of fibrillar material with many enmeshed erythrocytes were associated with these attachment sites. When adult worms became removed after fixation, large numbers of erythrocytes could be seen at the base of the ulcers. Examination of the morphological details of adultsA. ceylanicum revealed the characteristic mouthparts of large outer ventral teeth and smaller inner teeth. The distance between adjacent transverse cuticular striations was measured as 5.2 μm. The wide separation of the externolateral ray from the closely related mediolateral and posterolateral rays was illustrated together with the functional relationship of male and female copulating worms.
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References
Biocca E (1951) OnAncylostoma braziliense (de Faria, 1910) and its morphological differentiation fromA. ceylanicum (Looss, 1911) J Helminthol 125:1–10
Carroll SM, Grove DI, Dawkins HJS, Mitchell GF, Whitten LK (1983) Infections with a Malaysian dog strain ofAncylostoma ceylanicum in outbred, inbred and immunocompromised mice. Parasitology 87:229–238
Carroll SM, Howse DJ (1984a) The anticoagulant effects of the hookwormAncylostoma ceylanicum: observations of human and dog bloodin vitro and infected dogsin vivo. Thromb Haemost 51:222–227
Carroll SM, Robertson TA, Papadimitriou JM, Grove DI (1984b) Transmission electron microscopical studies of the site of attachment ofAncylostoma ceylanicum to the small bowel mucosa of the dog. J Helminthol (in press)
Graham R, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Gusnard D, Kirschner RH (1977) Cell and organelle shrinkage during preparation for scanning electron microscopy: effects of fixation dehydration and critical point drying. J Microsc 110:51–56
Kakofen UP (1970) Attachment and feeding behavior ofAncylostoma caninum. Z Parasitenkd 33:339–354
Kalkofen UP (1974) Intestinal trauma resulting from feeding activities ofAncylostoma caninum. Am J Trop Med Hyg 23:1046–1053
Lane C (1913) Ancylostomes and ancylostomiasis in Bengal. Indian Med Gaz 48:417–423
Lane C (1916) The genusAncylostoma in India and Ceylon. Indian J Med Res 4:74–92
Levine ND (1980) Nematode parasites of domestic animals and of man, 2nd edn. Burgess Publishing Company, Minneapolis
Sheehy TW, Meroney WH, Cox RS Jr, Soler JE (1962) Hookworm disease and malabsorption. Gastroenterology 42:148–156
Tandon BN, Kohli RK, Saraya AK, Ramachrandram K, Prakash O (1969) Role of parasites in the pathogenesis of intestinal malabsorption in hookworm disease. Gut 10:293–298
Thompson RCA, Dunsmore JD, Hayton AR (1979)Echinococcus granulosus: Secretory activity of the rostellum of the adult cestodein situ in the dog. Exp Parasitol 48:144–163
Yoshida Y, (1971) Comparative studies onAncylostoma braziliense andAncylostoma ceylanicum. 1. The adult stage. J Parasitol 57:983–989
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Carroll, S.M., Robertson, T.A., Papadimitriou, J.M. et al. Scanning electron microscopy ofAncylostoma ceylanicum and its site of attachment to the small intestinal mucosa of the dog. Z. Parasitenkd. 71, 79–85 (1985). https://doi.org/10.1007/BF00932921
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DOI: https://doi.org/10.1007/BF00932921