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High-performance liquid chromatographic analysis of monoamines in the cestodeDiphyllobothrium dendriticum

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Abstract

The presence of biogene monoamines in adult and larvalDiphyllobothrium dendriticum (Cestoda) was investigated by high-performance liquid chromatography with electrochemical detection (HPLC-ED). The biogene amines serotonin (5-HT), dopamine (DA), noradrenaline (NA), and adrenaline (A) as well as many of their precursors and metabolites, comprising a total of 15 different substances, were analyzed. 5-HT, DOPA, DA, NA, and A were detected in the worm, with 5-HT, DOPA, and DA being the dominating amines. The DA metabolites DOPAC and 3-MT or the 5-HT precursor 5-hydroxytryptophan could not be detected, but two unidentified substances, believed to be catecholic, were present in the worm. A high concentration of DOPA was measured in the proglottids and especially in the eggs. This is the first report of A in a flatworm.

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Authors and Affiliations

  1. Department of Biology, Åbo Akademi University, Åbo, Finland

    Krister Eriksson & Margaretha Gustafsson

  2. Department of Biochemistry and Pharmacy, Åbo Akademi University, Åbo, Finland

    Göran Åkerlind

Authors
  1. Krister Eriksson
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  2. Margaretha Gustafsson
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  3. Göran Åkerlind
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Eriksson, K., Gustafsson, M. & Åkerlind, G. High-performance liquid chromatographic analysis of monoamines in the cestodeDiphyllobothrium dendriticum . Parasitol Res 79, 699–702 (1993). https://doi.org/10.1007/BF00932514

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  • DOI: https://doi.org/10.1007/BF00932514

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