Abstract
The midgut cells of cephalobaenid pentastomids contain spherocrystals varying in size and appearance between the genera. Iron was detected in the vicinity of the crystals in all three genera, being considerably fainter inReighardia sternae than in the other species. Ultrastructurally a distinct lamination of the spherocrystals was evident, being faint inR. sternae but clearly expressed inRaillietiella hemidactyli andCephalobaena tetrapoda. According to the species, their diameter ranged from 1.3 to 6.25 μm. The size and number of the crystals were highest inR. hemidactyli and lowest inR. sternae. The inclusions were formed in the endoplasmic reticulum and migrated toward the cellular apex, accumulating there and being expelled into the midgut lumen. As determined by energy-dispersive X-ray analysis, calcium turned out to be the main component of the crystals. Furthermore, small amounts of chlorine and iron could be traced in the crystals. The relevance of the crystals is regarded as a kind of storage excretion such as that present in many other arthropods.
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References
Al-Mohanna SY, Nott JA (1987) R-cells and the digestive cycle inPenaeus semisulcatus (Crustacea: Decapoda). Mar Biol 95: 129–137
Anton-Erxleben F (1981) Investigations on the cuticle of the polychaete elytra using energy dispersive X-ray analysis. Helgol Wiss Meeresunters 34:439–450
Ballan-Dufrançais C (1972) Ultrastructure de l'iléon deBlatella germanica L. (Dictyoptère). Localisation, genèse et composition des concrétions minérales intracytoplasmiques. Z Zellforsch 133:163–179
Becker A, Peters W (1985) Fine structure of the midgut gland ofPhalangium opilio (Chelicerata, Phalangida). Zoomorphology 195:317–325
Doucet J (1965) Contribution à l'étude anatomique, histologique et histochimique des pentastomes (Pentastomida). Mem ORSTOM (Paris) 14:1–150
Ehrhardt P (1965) Magnesium und Calcium enthaltende Einschlusskörper in den Mitteldarmzellen von Aphiden. Experientia 21:337–338
Fain-Maurel M-A, Cassier P, Alibert J (1973) Étude infrastructurale et cytochimique de l'intestin moyen dePetrobius maritimus Leach en rapport avec ses fonctions excrétrices et digestives. Tissue Cell 5:603–631
Filshie BK, Poulson DF, Waterhouse DF (1971) Ultrastructure of the copper-accumulating region of theDrosophila larval midgut. Tissue Cell 3:77–102
Freeman BM (ed) (1984) Physiology and biochemistry of the domestic fowl, vol 5. Academic Press, London, pp 410–412
Gouranton J (1968) Composition, structure et mode de formation des concrétions minérales dans l'intestin moyen des Homoptères Cercopides. J Cell Biol 37:316–328
Goyffon M, Martoja R (1983) Cytophysiological aspects of digestion and storage in the liver of a scorpion,Androctonus australis (Arachnida). Cell Tissue Res 228:661–675
Greven H (1976) Some ultrastructural observations on the midgut epithelium ofIsohypsibius augusti (Murray, 1907) (Eutardigrada). Cell Tissue Res 166:339–351
Herman WS, Preus DM (1972) Ultrastructure of the hepatopancreas and associated tissues of the chelicerate arthropod,Limulus polyphemus. Z Zellforsch 134:255–271
Heymons R (1935) Pentastomida. In: Bronn HG (ed) Klassen und Ordnungen des Tierreichs, Bd 5. Akademische Verlagsgesellschaft, Leipzig, pp 1–268
Hopkin SP, Nott JA (1979) Some observations on concentrically structured, intracellular granules in the hepatopancreas of the shore crabCarcinus maenas (L.). J Mar Biol Assoc UK 59: 867–877
Hryniewiecka-Szyfter Z (1972) Ultrastructure of hepatopancreas ofPorcellio scaber Latr. in relation to the function of iron and copper accumulation. Bull Soc Amis Sci Lett Pozman Ser D 12/13:135–142
Hubert M (1978) Données histophysiologiques complémentaires sur les bioaccumulations minérales et puriques chezCylindroiulus londinensis (Leach, 1814) (Diplopode, Iuloidea). Arch Zool Exp Gen 119:669–683
Humbert W (1978) Cytochemistry and X-ray microprobe analysis of the midgut ofTomocerus minor Lubbock (Insecta, Collembola) with special reference to the physiological significance of the mineral concretions. Cell Tissue Res 187:397–416
Jeantet A-Y, Martoja R, Truchet M (1974) Rôle des sphérocristaux dans l'épithélium intestinal dans la résistance d'un Insecte aux pollutions minérales: données expérimentales obtenues par utilisation de la microsonde électronique et du micro-analyseur par émission ionique secondaire. C R Acad Sci [III] 278: 1441–1444
Jeschikov J (1940) Zur Morphologie vonPentastomum denticulatum. Zool Anz 132:263–279
Jimenez DR, Gilliam M (1988) Cytochemistry of peroxisomal enzymes in microbodies of the midgut of the honey bee,Apis mellifera. Comp Biochem Physiol [B] 90:757–766
Leuckart R (1860) Bau und Entwicklungsgeschichte der Pentastomen nach Untersuchungen besonders vonPent. taenioides undPent. denticulatum. C.F. Winter'sche Verlagsbuchhandlung, Leipzig, pp 1–160
Lohrmann E (1889) Untersuchungen über den anatomischen Bau der Pentastomen. Arch Naturgesch 1:303–337
Martoja R (1971) Données préliminaires sur les accumulations de sels minéraux et de déchets du catabolisme dans quelques organes d'Arthropodes. C R Acad Sci [III] 273:368–371
Martoja R (1972) Données histophysiologiques sur les accumulations minérales et puriques des Thysanoures (Insectes, Aptérygotes). Arch Zool Exp Gen 113:565–578
Rao KH, Jennings JB (1959) The alimentary system of a pentastomid from the Indian water-snakeNatrix piscator Schneider. J Parasitol 45:299–300
Riley J (1972) Histochemical and ultrastructural observation on feeding and digestion inReighardia sternae (Pentastomida: Cephalobaenida). J Zool 167:307–318
Romeis B (1968) Mikroskopische Technik, 16. Auflage. R. Ouldenbourg, München Wien
Romeis B (1989) Mikroskopische Technik, 17. Auflage. Urban und Schwarzenberg, München Wien Baltimore
Schultz TW (1976) The ultrastructure of the hepatopancreatic caeca ofGammarus minus (Crustacea, Amphipoda). J Morphol 149:383–400
Stiles CW (1891) Bau und Entwicklungsgeschichte vonPentastomum proboscideum Rud. undPentastomum subcylindricum Dies. Z Wiss Zool 52:85–157
Storch V, Holm P, Ruhberg H (1988) Zur Ultrastruktur und Histologie des Darmkanals verschiedener Onychophora. Zool Anz 221:281–294
Thomas G, Böckeler W (1992) Light and electron microscopical investigations of the midgut epithelium of different Cephalobaenida (Pentastomida) during digestion. Parasitol Res 78: 587–593
Walker G (1977) “Copper” granules in the barnacleBalanus balanoides. Mar Biol 39:343–349
Wessing A, Eichelberg D (1978) Malpighian tubules, rectal papillae and excretion. In: Ashburner M, Novitski E (eds) The genetics and biology ofDrosophila, vol 2. Academic Press, New York San Francisco, chap 19, pp 1–41
Wigglesworth VB (1943) The fate of haemoglobin inRhodnius prolixus (Hemiptera) and other blood-sucking arthropods. Proc R Soc Lond [Biol] 131:313–339
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Thomas, G., Böckeler, W. Investigation of the intestinal spherocrystals of different Cephalobaenida (Pentastomida). Parasitol Res 80, 420–425 (1994). https://doi.org/10.1007/BF00932380
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DOI: https://doi.org/10.1007/BF00932380