Abstract
Exoerythrocytic stages ofPlasmodium berghei cultured in HepG2-A16 hepatoma cells and those ofP. falciparum in human hepatocytes transplanted under the kidney capsule of CB-17/ICr scid/scid mice were used to evaluate expression of heat-shock-related stress proteins. Although undetectable in the sporozoites, the expression of proteins similar in sequence of a heat-shock protein of 70 kDa and a glucose-regulated protein of 78 kDa was markedly induced in the hepatic stages of malaria parasites. Expression of these proteins in the exoerythrocytic stages of the malaria parasite warrants a systematic evaluation of their potential role in eliciting cellular immune responses directed against infected hepatocytes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aikawa M, Atkinson CT (1990) Immunoelectron microscopy of parasites. Adv Parasitol 29:151–214
Ardeshir F, Flint JE, Richman S, Reese RT (1987) A 75 kDa merozoite surface protein ofPlasmodium falciparum which is related to the 70 kDa heat-shock protein. EMBO J 6:493–499
Atkinson CT, Aikawa M, Aley SB, Hollingdale MR (1989) Expression ofPlasmodium berghei circumsporozoite antigens on the surface of exoerythrocytic schizonts and merozoites. Am J Trop Med Hyg 41:9–17
Bianco AE, Favaloro JM, Burkot TR, Culvenor JG, Crewther PE, Brown GV, Anders R, Coppel RL, Kemp DJ (1986) A repetitive antigen ofPlasmodium falciparum that is homologous to heat shock protein 70 ofDrosophila melanogaster. Proc Natl Acad Sci USA 83:8713–8717
Ferone R, Burchall JJ, Hitching GH (1969)Plasmodium berghei dihydrofolate reductase. Isolation, properties, and inhibition by antifolates. Mol Pharmacol 5:49–59
Good MF, Miller LH (1990) T-cell antigens and epitopes in malaria vaccine design. Curr Top Microbiol Immunol 155:65–78
Hollingdale MR, Leland P, Schwartz AL (1983) In vitro cultivation of exoerythrocytic stage ofPlasmodium berghei in a hepatoma cell line. Am J Trop Med Hyg 32:682–684
Hollingdale MR, Aikawa M, Atkinson CT, Ballou WR, Chen G, Meis JFGM, Sina B, Zhu J (1990) Non-CS pre-erythrocytic protective antigens. Immunol Lett 25:71–76
Khusmith S, Charoenvit Y, Kumar S, Sedegah M, Beaudoin RL, Hoffman SL (1991) Protection against malaria by vaccination with sporozoite surface protein 2 plus CS protein. Science 252:715–718
Kumar N, Zheng H (1992) Nucleotide sequence of aPlasmodium falciparum stress protein with similarity to mammalian 78 kDa glucose-regulated protein. Mol Biochem Parasitol 56:353–356
Kumar N, Syin C, Carter R, Quakyi IA, Miller LH (1986) Identification and expression of heat shock genes infalciparum malaria. J Cell Biol 103:176a
Kumar N, Syin C, Carter R, Quakyi IA, Miller LH (1988)Plasmodium falciparum gene encoding a protein similar in sequence to the 78 kDa rat glucose-regulated stress protein. Proc Natl Acad Sci USA 85:6277–6281
Kumar N, Zhao Y, Graves P, Folgar JP, Maloy L, Zheng H (1990) Human immune response directed againstPlasmodium falciparum heat shock-related proteins. Infect Immun 58:1408–1414
Kumar N, Koski G, Harada M, Aikawa M, Zheng H (1991) Induction and localization ofPlasmodium falciparum stress proteins related to the heat shock protein 70 family. Mol Biochem Parasitol 48:47–58
Mazier D, Renia L, Nussler A, Pied S, Marussig M, Goma J, Grillot D, Miltgen F, Drapier J, Corradin G, Giudice GD, Grau GE (1990) Hepatic phase of malaria is the target of cellular mechanisms induced by the previous and the subsequent stages: a crucial role for liver nonparenchymal cells. Immunol Lett 25:65–70
Oaks SG Jr, Mitchell VS, Pearson GW, Carpenter CCJ (1991) Vaccines. In: Malaria-obstacles and opportunities, ch 9. National Academic Press, Washington, DC, pp 169–210
Renia L, Mattei D, Goma J, Pied S, Dubois P, Miltgen F, Nussler A, Matile H, Menegaux F, Gentilini M, Mazier D (1990) A malaria heat-shock like determinant expressed on the infected surface is the target of antibody-dependent cell-mediated cytotoxic mechanisms by nonparenchymal liver cells. Eur J Immunol 20:1445–1449
Sacci JB, Schriefer ME, Resau JH, Wirtz RA, Detolla LJ, Markham RB, Azad AF (1992) Mouse model for exoerythrocytic stages ofPlasmodium falciparum malaria parasite. Proc Natl Acad Sci USA 89:3701–3705
Yang Y-F, Tan-ariya P, Sharma YD, Kilejian A (1987) The primary structure of aPlasmodium falciparum polypeptide related to heat shock proteins. Mol Biochem Parasitol 26:61–68
Yoshida N, Nussenzweig RS, Potocnjak P, Nussenzweig V, Aikawa M (1980) Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite. Science 207:71–73
Zhu JD, Waters AP, Appiah A, McCutchan TF, Lal AA, Hollingdale MR (1990) Stage-specific ribosomal RNA expression switches during sporozoite invasion of hepatocytes. J Biol Chem 265:12740–12744
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kumar, N., Nagasawa, H., Sacci, J.B. et al. expression of members of the heat-shock protein 70 family in the exoerythrocytic stages ofPlasmodium berghei andPlasmodium falciparum . Parasitol Res 79, 109–113 (1993). https://doi.org/10.1007/BF00932255
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00932255