Abstract
A precocious line ofEimeria intestinalis was obtained by selection for early development of oocysts in rabbits and after six consecutive passages in animals. This line (EiP) was derived from a wild strain (EiO) isolated in 1975 from the caecal content of a rabbit with coccidiosis. The prepatent period of the EiP strain was reduced from 215 h to<144 h, the result being that the oocyst sporulation time was the same for both lines. The excreted and unsporulated oocysts had exactly the same shape, but microscopical examination of the sporulated oocysts showed a marked difference between EiP and EiO strains. A huge refractile globule was located in each of two sporocysts of the precocious line, whereas no refractile globule was seen in the other two. The EiP line had a reproductive potential much lower (1000 times) than that of its parent strain EiO and, as judged by the weight gain, mortality and lesions that also occurred in the jejunum and above all in the ileum, its pathogenicity was substantially reduced.
Similar content being viewed by others
References
Bedrnik P, Kucera J, Jurkovic P, Firmanova A (1986) Results of experiments on pathogenicity and immunization with selected lines ofE. tenella In: McDougald R, Joyner P, Long PL (eds) Research in avian coccidiosis. Proceedings of the Georgia Coccidiosis Conference, November 19–21, Atlanta, pp 510–525
Catchpole J, Norton CC (1979) The species ofEimeria in rabbits for meat production in Britain. Parasitology 79:249–257
Cheissin EM (1948) Ravitie dvuh kisecnyh kokcidij krolika-Eimeria piriformis Kotlan u. Popesch iEimeria intestinalis nom. nov. Uch Zap Karelo-Fin Gos Univ Biol Nauti 3:179–187
Coudert P (1976) Les coccidies intestinales du lapin: comparison du pouvoir pathogène d'E. intestinalis avec trois autresEimeria. CR Acad Sci Ser D Paris 282:2219–2222
Coudert P (1979) Comparison of pathology of several rabbit Coccidia species and their control with robenidine. In: Proceedings of the International Symposium on Coccidia and Further Prospects of Their Control, Prague, November 1979
Coudert P, Yvore P, Provot F (1973) Sporogonie d'E. stiedai (Lindermann 1865, Kisskalt et Hartmann 1907). Influence de la température sur la respiration et sur la durée de la segmentation. Ann Rech Vet 4:371–388
Coudert P, Licois D, Streun A (1979) Characterization ofEimeria species: 1. Isolation and study of pathogenicity of a pure strain ofEimeria perforans (Leuckart 1879; Sluiter and Swellengrebel 1912). Z Parasitenkd 59:227–234
Coudert P, Licois D, Besnard J (1988) Establishment of a specified pathogen free breeding colony (SPF) without hysterectomy and Hand-rearing procedures. In: proceedings of the 4th Congress of the World Rabbit Science Association, October 10–14, Budapest, pp 137–148
Fioramonti J, Sorraing JM, Licois D, Bueno L (1981) Intestinal motor and transit disturbances associated with experimental coccidiosis (Eimeria magna) in the rabbit. Ann Rech Vet 12:413–420
Jeffers TK (1975) Attenuation ofEimeria tenella through selection for precociousness. J Parasitol 61:1083–1090
Jeffers TK (1986) Attenuation of coccidia. A review. In: McDougald R, Joyner P, Long PL (eds) Research in avian coccidiosis. Proceedings of the Georgia Coccidiosis Conference, November 19–21, Atlanta, pp 482–497
Johnson JK, Long PL, McKenzie ME (1986) The pathogenicity, immunogenicity and endogenous development of a precocious line ofEimeria brunetti. Avian Pathol 15:697–704
Licois D, Coudert P (1980a) Attempt to suppress immunity in rabbits immunized againstEimeria intestinalis. Ann Rech Vet 11:273–278
Licois D, Coudert P (1980b) Action de la Robenidine sur l'excrétion des oocystes de différentes espèces de coccidies du lapin. Rec Med Vet 156:391–394
Licois D, Coudert P (1982) Coccidioses et diarrhées du lapin à l'engraissement. Bull GTV 5:109–122
Licois D, Coudert P, Mongin P (1978a) Changes in hydromineral metabolism in diarrhoeic rabbits: 1. A study of the changes in water metabolism. Ann Rech Vet 9:1–10
Licois D, Coudert P, Mongin P (1978b) Changes in hydromineral metabolism in diarrhoeic rabbits: 2. Study of the modifications of electrolyte metabolism. Ann Rech Vet 9:453–464
Long PL, Rose ME (1982) Prospects for the control of coccidiosis by immunization. World's Poult Sci J 38:85–96
McDonald V, Ballingall S (1983a) Attenuation ofEimeria mivati (=mitis) by selection for precocious development. Parasitology 86:371–379
McDonald V, Ballingall S (1983b) Further investigation of the pathogenicity, immunogenicity and stability of precociousEimeria acervulina. Parasitology 86:361–369
McDonald V, Shirley MW (1986) The asexual development of precocious lines ofEimeria spp. in the chicken. In: McDougald R, Joyner P, Long PL (eds) Research in avian coccidiosis. Proceedings of the Georgia Coccidiosis Conference, November 19–21, Atlanta, pp 502–509
McDonald V, Ballingall S, Shirley MW (1982) A preliminary study of the nature of infection and immunity in chickens given an attenuated line ofEimeria acervulina. Parasitology 84:21–30
McDonald V, Shirley MW, Bellatti MA (1986)Eimeria maxima: characteristics of attenuated lines obtained by selection for precocious development in the chicken. Exp Parasitol 61:192–200
McDougald LR, Jeffers TK (1976)Eimeria tenella (Sporozoa, Coccidia): gametogony following a single asexual generation. Science 192:258–259
Norton CC, Chard MJ (1983) The oocyst sporulation time ofEimeria species from the fowl. Parasitology 86:193–198
Peeters JE, Geeroms R, Froyman R, Halen P (1981) Coccidiosis in rabbits: a field study. Res Vet Sci 30:328–334
Peeters JE, Charlier G, Antoine O, Mammerickx M (1984) Clinical and pathological changes afterEimeria intestinalis infection in rabbits. Zentralbl Veterinaermed Reihe B 31:9–24
Pellerdy L (1954)Eimeria agnosta n. sp. from the rabbit. Acta Vet Acad Sci Hung 4:259–261
Rose ME (1982) Host immune responses. In: Long PL (ed) The biology of the Coccidia. University Park Press, Baltimore, pp 329–371
Rose ME (1986) Immune responses toEimeria infection. In: McDougald R, Joyner P, Long PL (eds) Research in avian coccidiosis. Proceedings of the Georgia Coccidiosis Conference, November 19–21, Atlanta, pp 449–469
Rose ME, Long PL (1980) Vaccination against coccidiosis in chickens. Symp Br Soc Parasitol 18:57–74
Schellenberg P (1976) Créations d'une souche de lapins “SPF”. 1er Congrès Int. Cunic. 31 mars-2 avril; Dijon. Commun. 38:1–4
Shirley MW (1983) Biology of a precocious (and attenuated) line ofEimeria necatrix. Parasitology 82(2):viii (Abstract)
Shirley MW, Bellatti MA (1984)Eimeria necatrix: selection and characteristics of a precocious (and attenuated) line. Avian Pathol 13:657–668
Shirley MW, McDonald V, Chapman HD, Millard BJ (1984)Eimeria praecox: selection and characteristics of precocious lines. Avian Pathol 13:669–682
Shirley MW, McDonald V, Bellatti MA (1986)Eimeria brunetti: selection and characteristics of a precocious line. Avian Pathol 15:705–717
Zundel E, Renault L, Coudert P (1980) Coccidioses intestinales du lapin. Sondage épidémologique. 2ème Congrès Int. cunic. 15–18 avril; Barcelone t II:308–314
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Licois, D., Coudert, P., Boivin, M. et al. Selection and characterization of a precocious line ofEimeria intestinalis, an intestinal rabbit coccidium. Parasitol Res 76, 192–198 (1990). https://doi.org/10.1007/BF00930814
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00930814