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A simple method for the determination of poly-β-hydroxybutyric acid in microbial biomass

  • Biotechnology and Industrial Microbiology
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Summary

Poly-β-hydroxybutyric (PHB) acid can be determined IR-spectrophotometrically following chloroform extraction from bacterial biomass. The limit of sensitivity with the present instrument was 50 μg/ml. Interferring triglycerides can be estimated by selective adsorption of PHBS on silica gel.

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References

  • Dawes,E.A., Senior,P.J. (1973). Adv. Microbial Physiol.10, 135–266

    Google Scholar 

  • Haynes,W.C., Melvin,E.H., Locke,J.M., Glass,C.A., Senti,F.R. (1958). Appl. Microbiol.6, 298–304

    PubMed  Google Scholar 

  • Norris,K.P., Greenstreet,J.E.S. (1958). J. gen. Microbiol.19, 566–580

    PubMed  Google Scholar 

  • Okamura,K., Marchessault,R.H. (1967). In: Conformation of Biopolymers, Ramachandran,G.M., ed. Vol. 2, pp. 907–920. New York: Academic Press

    Google Scholar 

  • Schlegel,H.G., Lafferty,R. (1961). Arch. Mikrobiol.38, 52–54

    PubMed  Google Scholar 

  • Thiele,O.W., Dreysel,J., Hermann, D. (1972). Eur.J.Biochem.29, 224–236

    PubMed  Google Scholar 

  • Wilde,E. (1962). Arch. Mikrobiol.43, 109–137

    PubMed  Google Scholar 

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Author notes

  1. R. M. Lafferty

    Present address: Technische Hochschule Graz, Institut für biochemische Technologie und Lebensmittelchemie, Schlögelgasse 9, Graz, Österreich

Authors and Affiliations

  1. Institut für Mikrobiologie der Gesellschaft für Strahlen- und Umweltforschung mbH, München, in Göttingen

    R. -R. Jüttner, R. M. Lafferty & H. -J. Knackmuss

  2. Institut für Mikrobiologie der Universität Göttingen, Göttingen, Germany

    R. -R. Jüttner, R. M. Lafferty & H. -J. Knackmuss

  3. Abt. für Bakterienphysiologie, Institut für Mikrobiologie, Grisebachstr. 8, D-3400, Göttingen-Weende, Germany

    H. -J. Knackmuss

Authors
  1. R. -R. Jüttner
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  2. R. M. Lafferty
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  3. H. -J. Knackmuss
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Additional information

Dedicated to Herrn Prof. Dr. H. Grubitsch, Vorstand des Institutes für Anorganisch-Chemische Technologie und Analytische Chemie der Technischen Hochschule Graz on the occasion of his 70th birthday.

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Jüttner, R.R., Lafferty, R.M. & Knackmuss, H.J. A simple method for the determination of poly-β-hydroxybutyric acid in microbial biomass. European J. Appl Microbiol. 1, 233–237 (1975). https://doi.org/10.1007/BF00930696

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  • DOI: https://doi.org/10.1007/BF00930696

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