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Separation of amastigotes and trypomastigotes ofTrypanosoma cruzi from cultured cells

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Abstract

A method is described for the isolation and purification of trypomastigotes and amastigotes ofTrypanosoma cruzi from cell cultures. L-A9, a transformed fibroblast cell line, and J774G8, a macrophage-like cell line of tumor origin, were used. Both cell lines were infected with bloodstream trypomastigotes ofT. cruzi, which one within host cells transform into dividing amastigotes. After 6–8 days infection the host cells ruptured, spontaneously liberating parasites into the culture medium. L-A9 cells liberated mainly trypomastigotes while J774G8 cells liberated amastigotes. The parasites were collected and purified by centrifugation in a gradient of metrizamide. The purity of the preparation as well as the morphology of the parasites and the host cells were analysed by electron microscopy.

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Authors and Affiliations

  1. Instituto de Biofisica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21941, Rio de Janeiro, RJ, Brazil

    Técia Ulisses de Carvalho & Wanderley de Souza

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  1. Técia Ulisses de Carvalho
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  2. Wanderley de Souza
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de Carvalho, T.U., de Souza, W. Separation of amastigotes and trypomastigotes ofTrypanosoma cruzi from cultured cells. Z. Parasitenkd. 69, 571–575 (1983). https://doi.org/10.1007/BF00926668

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  • DOI: https://doi.org/10.1007/BF00926668

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