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Retinoid dynamics in chicken eye during pre-and postnatal development

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Abstract

Changes in the steady state level of retinols, retinaldehydes and retinyl esters in the trans and 11-cis forms and trans retinoic acid were measured in whole chicken eye during development from day 6in ovo to day 3 post-hatch. These retinoids, quantified by different HPLC systems, were detected in this time sequence: trans-retinol and trans-retinyl esters in the first weekin ovo, 11-cis-retinol in the second week. The highest level of 11-cis-retinaldehyde and 11-cis-retinyl esters was reached at the end of developmentin ovo; however, their levels increased further after hatching. The retinoic acid level decreased at the end of the first week, rising again at the end of the second week.

The enzyme activities involved in the metabolism of these retinoids-acyl-CoA: retinol acyltransferase, trans-retinol dehydrogenase, 11-cis-retinol dehydrogenase, trans-retinyl ester hydrolase and trans: 11-cis-retinol isomerase were also estimated and they were detectable already in the first week of developmentin ovo.

At day 6 of the biosynthesis of retinoic acid by the retinaldehyde dehydrogenase activity from retina cytosol was also shown.

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Authors and Affiliations

  1. Istituto di Chimica Biologica, Facoltà di Medicina e Chirurgia, Policlinico, Università di Palermo, Via del Vespro 129, 90127, Palermo, Italy

    Concetta M. A. Nicotra, Maria C. Gueli, Grazia De Luca, Antonino Bono, Anna M. Pintaudi & Alessandra Paganini

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  1. Concetta M. A. Nicotra
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  2. Maria C. Gueli
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  3. Grazia De Luca
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  4. Antonino Bono
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  5. Anna M. Pintaudi
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  6. Alessandra Paganini
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Nicotra, C.M.A., Gueli, M.C., De Luca, G. et al. Retinoid dynamics in chicken eye during pre-and postnatal development. Mol Cell Biochem 132, 45–55 (1994). https://doi.org/10.1007/BF00925674

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  • DOI: https://doi.org/10.1007/BF00925674

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