Abstract
Two monoclonal antibodies reacted with theTrypanosoma cruzi-specific antigen of an apparent Mr 25,000 from all developmental forms (Tachibana et al. 1986). ThisT. cruzi-specific antigen was found at the plasma membrane by immunoperoxidase electron microscopy using the monoclonal antibodies TCF48 and TCF87. The TCF48 and TCF87-treated cells showed stain deposits at the plasma membrane clearly distinguishable from those in cells treated with a monoclonal antibody against a surface antigen. This suggests that the epitope(s) of the Mr 25,000 antigen is located on the inner surface or in the matrix of the plasma membrane. TCF48 and TCF87 also reacted with an antigen on the microtubules of the axoneme, but not with the subpellicular microtubules. These results suggest that theT. cruzi-specific Mr 25,000 antigen is common to both the plasma membrane and axoneme but it is not located at the subpellicular microtubules. Its identity and that of the surface antigen, Gp25 (Scharfstein et al. 1983) as well as its role in the pathogenicity of the parasite are discussed.
Similar content being viewed by others
References
Afchain D, Le Ray D, Fruit J, Capron A (1979) Antigenic make-up ofTrypanosoma cruzi culture forms: Identification of a specific component. J Parasitol 65:507–514
Andrews NW, Katzin AM, Colli W (1984) Mapping of surface glycoproteins ofTrypanosoma cruzi by two-dimensional electrophoresis. Eur J Biochem 140:599–604
Gallo J-M, Anderton BH (1983) A subpopulation of trypanosome microtubules recognized by a monoclonal antibody to tubulin. EMBO J 2:479–483
Grögl M, Kuhn RE (1985) Identification of antigens ofTrypanosoma cruzi which induce antibodies during experimental Chagas' disease. J Parasitol 71:183–191
Lanar DE, Manning JE (1984) Major surface proteins and antigens on the different in vivo and in vitro forms ofTrypanosoma cruzi. Mol Biochem Parasitol 11:119–131
Mendonça-Previato L, Gorin PAJ, Braga AF, Scharfstein J, Previato JO (1983) Chemical structure and antigenic aspects of complexes obtained from epimastigotes ofTrypanosoma cruzi. Biochemistry 22:4980–4987
Mohri H (1976) The function of tubulin in motile systems. Biochim Biophys Acta 456:85–127
Piras MM, De Rodriguez OO, Piras R (1981)Trypanosoma cruzi: Antigenic composition of axonemes and flagellar membranes of epimastigotes culturedin vitro. Exp Parasitol 51:59–73
Santos-Buch CA, Acosta AM, Zweerink HJ, Sadigursky M, Andersen OF, von Kreuter BF, Brodskyn CI, Sadigursky C, Cody RJ (1985) Primary muscle disease: Definition of a 25-kDa polypeptide myopathic specific Chagas antigen. Clin Immunol Immunopathol 37:334–350
Scharfstein J, Rodrigues MM, Alves CA, De Souza W, Previato JO, Mendonça-Previato L (1983)Trypanosoma cruzi: Description of a highly purified surface antigen defined by human antibodies. J Immunol 131:972–976
Scharfstein J, Luquetti A, Murta ACM, Senna M, Rezende JM, Rassi A, Mendonça-Previato L (1985) Chagas' disease: Serodiagnosis with purified Gp25 antigen. Am J Trop Med Hyg 34:1153–1160
Snary D, Hudson L (1979)Trypanosoma cruzi cell surface proteins: Identification of one major glycoprotein. FEBS Lett 100:166–170
Tachibana H, Kaneda Y (1984) Monoclonal antibodies toTrypanosoma cruzi: Characterization of specific antigens in epimastigote stage. Jpn J Parasitol 33:457–466
Tachibana H, Nagakura K, Kaneda Y (1986) Species-specific monoclonal antibodies for a membrane antigen(s) in all developmental forms ofTrypanosoma cruzi. Z Parasitenkd 72:433–441
Wilson MB, Nakane PK (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In: Knapp W, Holubar K, Wick G (eds), Immunofluorescence and related staining techniques, Elsevier/North-Holland Biomedical Press, pp 215–224
Zweerink HJ, Anderson OF, Greenblatt HC, Murray PK (1985) Parasite specific antibodies in three strains of mice after infection withTrypanosoma cruzi. J Parasitol 71:43–49
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tachibana, H., Montenegro, L.T., Kurihara, K. et al. Localization of theTrypanosoma cruzi-specific Mr 25,000 antigen by immune electron microscopy using monoclonal antibodies. Z. Parasitenkd. 72, 701–707 (1986). https://doi.org/10.1007/BF00925091
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00925091