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Bone-resorbing activity is expressed by rat macrophages in response to arthropathic streptococcal cell wall polymers

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Abstract

Rat peritoneal macrophages stimulated in vivo by group A streptococcal peptidoglycan-polysaccharide (PG-APS) resorb bone as measured by solubilization of45Ca from radiolabeled, devitalized bone chips. Activity was strain-dependent and correlated with the susceptibility of rat strains to PG-APS-induced arthritis. PG-APS-stimulated macrophages from the resistant Buf rat strain were not induced to resorb bone, but ingested equivalent concentrations of PG-APS compared to bone-resorbing macrophages from the arthritis-susceptible Lew strain. Resorptive activity peaked at three to five days and decreased to background levels by 10 days after injection. PG-APS-stimulated macrophages from congenitally athymic Lew rats were as effective as macrophages from heterozygous littermates at resorbing bone. Lew macrophages were also responsive to small, nonarthropathic PG-APS polymers generated by mutanolysin digestion. Resident peritoneal macrophages did not respond to stimulation by PG-APS in vitro. Indomethacin at a concentration of 10 μg/ml was an effective blockade against PG-APS-induced macrophage bone resorption in vitro, but catalase was ineffective. These results indicate that expression of rat macrophage bone-resorbing activity reflects genetic regulation of the response to PG-APS rather than a defect in ingestion of these polymers and imply that PG-APS-stimulated, bone-resorbing macrophages may contribute to early, initial bone destruction that occurs in inflammatory arthritis.

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  1. Lynn A. Bristol-Rothstein

    Present address: National Cancer Institute, National Institutes of Health, Building 10, Room 4B05, 20892, Bethesda, Maryland

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of North Carolina, 27599, Chapel Hill, North Carolina

    Lynn A. Bristol-Rothstein & John H. Schwab

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  1. Lynn A. Bristol-Rothstein
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Bristol-Rothstein, L.A., Schwab, J.H. Bone-resorbing activity is expressed by rat macrophages in response to arthropathic streptococcal cell wall polymers. Inflammation 16, 485–496 (1992). https://doi.org/10.1007/BF00918974

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