Abstract
Superoxide dismutase (SOD) is known to regulate the level of superoxide radicals inside cells. The purpose of this work was to investigate the role of SOD activity in tissue damage produced by superoxide radicals. SOD was measured in polymorphonuclear cells of patients with rheumatoid arthritis and controls. The distinct SOD activities, including manganese-containing and copperzinc-containing enzymes, were evaluated in cytoplasma and mitochondria of human granulocytes. Except for the comparison between total SOD and cytoplasmic copper-zinc SOD, no correlation was found among the different SOD levels. Moreover, a significant decrease was observed only for cytoplasmic manganesecontaining enzyme in granulocytes of adults with rheumatoid arthritis. These data confirm the necessity of evaluation of various SOD classes and suggest the interest of biochemical tests in granulocytes for early diagnosis and better comprehension of tissue damage due to inflammation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Babior, B. B., R. S. Kipnes, andJ. T. Curnutte. 1973. Biological defense mechanism. The production by leukocytes of superoxide, a potential bactericidal agent.J. Clin. Invest. 52:741–744.
Johnston, R. B., andE. Lehmeyer. 1976. Elaboration of toxic oxygen by products by neutrophils in a model of immune complex disease.J. Clin. Invest. 57:836–841.
Mac Phail, L. C., P. M. Henson, andR. B. Johnston. 1981. Respiratory burst enzyme in human neutrophils.J. Clin. Invest. 67:710–716.
Beckman, G., E. Lundgren, andA. Tarnviok. 1973. Superoxide dismutase isoenzymes in different human tissues, their genetic control and intracellular localization.Hum. Hered. 23:338–345.
Johnston, R. B., andL. R. De Chatelet. 1974. Protection against superoxide anion.N. Engl. J. Med. 290:1383.
McCord, J. M. 1974. Free radicals and inflammation: protection of synovial fluids by superoxide dismutase.Science 185:529–531.
Rister, M., K. Bavermeister, U. Gravert, andE. Gladika. 1978. Superoxide dismutase deficiency in rheumatoid arthritis.Lancet 1:1094.
Böyum, A. 1968. Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation and of granulocytes by combining centrifugation and sedimentation at 1 g.Scand. J. Clin. Lab. Invest. 215:77–89.
Salin, M. L., andJ. M. McCord. 1974. Superoxide dismutases in polymorphonuclear leukocytes.J. Clin. Invest. 54:1005–1009.
Greenwald, R. A., andW. W. Moy. 1979. Inhibition of collagen gelation by action of the superoxide radical.Arthritis Rheum. 22:251–259.
Gordon, P., S.D. Stulberg, andS. M. Sherer. 1980. Suppression of experimental inflammatory arthritis by an immunomodulation, therafectin which inhibits the egress of superoxides from neutrophils.Arthritis Rheum. 23(6):683–684.
Menander-Hubert, K. B., andW. Huber. 1977. Orgotein, the drug version of bovine Cu-Zn superoxide dismutase. II-A summary account of clinical trials in man and animals. In Superoxide and superoxide dismutase. Michelson, A.M., McCord, J.M., and Fridarch, I. editors. Academic Press, New York. 537–549.
Goldstein, I. M., M. Cerqueira, S. Lind, andH. B. Kaplan. 1977. Evidence that the superoxide generating system of human leukocytes is associated with the cell surface.J. Clin. Invest. 59:249–254.
Del Villano, B. C., S. I. Miller, L. P. Schacter, andJ. A. Tischfield. 1980. Elevated superoxide dismutase in black alcoholics.Science 207:991–993.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pasquier, C., Mach, P.S., Raichvarg, D. et al. Manganese-containing superoxide-dismutase deficiency in polymorphonuclear leukocytes of adults with rheumatoid arthritis. Inflammation 8, 27–32 (1984). https://doi.org/10.1007/BF00918350
Issue Date:
DOI: https://doi.org/10.1007/BF00918350