Abstract
Cord blood samples from healthy full-term newborns were tested with antimature and antiimmature lymphoid-cell monoclonal antibodies, as well as more traditional markers, in order to identify the phenotype of circulating precursor cells. The results demonstrated that human cord blood contains a lower number of OKT3+, E-rosetting mature T cells than adult blood, very high levels of OKT10+ cells, and few OKT9+, OKT8+OKT3−, and OKT4+OKT3− cells. Although the finding of OKT9+ and OKT10+ cord circulating cells could be indicative of cell activation, double marker studies in newborn blood pointed to phenotypically immature lymphoid subsets at different stages of maturation, according to Reinherz's hypothesis. In addition, the absence of nuclear Tdt-positive and hot-rosetting cells, together with the fact that most of these are OKT3+, OKT10+, OKT4+, or OKT8+ cells, suggests that the surface phenotype of newborn lymphocytes is similar to that of mature thymocytes.
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Foa R, Catovsky D, Cherchi M, Benavides I, Ganeshaguru K, Hoffbrand AV: Cell surface and enzyme markers of cord blood lymphocytes. Br J Haematol 44:583, 1980
Sridama V, Pacini F, Yang SL, Moawad A, Reilly M, De Groot J: Decreased levels of helper T cells: A possible cause of immunodeficiency in pregnancy. N Engl J Med 307:352, 1982
Maccario R, Nespoli L, Mingrat G, Vitiello A, Ugazio AG, Burgio GR: Lymphocyte subpopulations in the neonate: Identification of an immature subset of OKT8-positive, OKT3-negative cells. J Immunol 130:1303, 1983
Gerli R, Rambotti P, Cernetti C, Velardi A, Spinozzi F: Evidence for phenotypic T precursors cells in human cord blood. Br J Haematol 53:685, 1983 (letter)
Reinherz EL, Schlossman SF: Regulation of the immune response-inducer and suppressor T-lymphocyte subsets in human beings. N Engl J Med 303:370, 1980
Wybran J, Carr MC, Fudenberg HH: The human rosette-forming cell as a marker of a population of thymus-derived cells. J Clin Invest 51:2537, 1972
Lay WH, Mendes NF, Bianco C, Nüssenzweig V: Binding of sheep red blood cells to a large population of human lymphocytes. Nature 230:531, 1971
Reinherz EL, Kung PC, Pesando JM, Ritz J, Goldstein G, Schlossman SF: Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression. J Exp Med 150:1472, 1979
Hercend T, Nadler LM, Pesando JM, Reinherz EL, Schlossman SF, Ritz J: Expression of a 26,000 dalton glycoprotein on activated human T cells. Cell Immunol 64:192, 1981
Edwards PA: Monoclonal antibodies that bind to the human erythrocyte membrane glycoproteins, glycophorin A and band 3. Biochem Soc Trans 8:334, 1980
Mannoni P, Janowska-Wieczorek A, Turner R, McGann L, Turc JM: Monoclonal antibodies against human granulocytes and myeloid differentiation antigens. Hum Immun 5:309, 1982
Vainchencker W, Deschamps JF, Bastin JM, Titeux M, Breton-Gorius J, McMichael AJ: Two monoclonal antiplatelet antibodies as markers of human megakaryiocyte maturation: Immunofluorescent staining and platelet peroxidase. Blood 59:514, 1982
Bach MA, Bach JF: The use of monoclonal anti-T cell antibodies to study T cell imbalances in human diseases. Clin Exp Immunol 45:449, 1981
Andersson U, Bird AG, Britton S, Palacios R: Humoral and cellular immunity in humans studied at the cell level from birth to two years of age. Immunol Rev 57:5, 1981
Hayward AR: Development of lymphocyte responses and interactions in the human fetus and newborn. Immunol Rev 57:39, 1981
Zola H, Moore MA, Bradley J, Need JA, Beverley PCL: Lymphocyte sub-populations in human cord blood: Analysis with monoclonal antibodies. J Reprod Immunol 5:311, 1983
Johnson C, Dwyer JM: Comparative analysis of the heterogeneity of mononuclear cells present in adult and cord blood by simultaneous examination of multiple phenotypic characteristics. Cell Immunol 81:88, 1983
Siegal FP: Functional ontogeny of human lymphoid cells as a factor in maternal-fetal tolerance. Am J Reprod Immunol 1:65, 1981
Gathings WE, Kubagawa H, Cooper MD: A distinctive pattern of B cell immaturity in perinatal humans. Immunol Rev 57:107, 1981
Mackler BF, Richie E, Swate T: T cell sub-population shifts in human cord blood high- and low-affinity E-RCF. Clin Immunol Immunopathol 15:238, 1980
Hayward AR, Kurnick J: Newborn T cell suppression: Early appearance, maintenance in culture, and lack of growth factor suppression. J Immunol 126:50, 1981
Yachie A, Miyawaki T, Nagaoki T, Yokoi T, Mukai M, Uwadana N, Taniguchi N: Regulation of B cell differentiation by T cell subsets defined with monoclonal OKT4 and OKT8 antibodies in human cord blood. J Immunol 127:1314, 1981
Asma GEM, Van Den Bergh R, Vossen JM: Use of monoclonal antibodies in a study of the development of T lymphocytes in the human fetus. Clin Exp Immunol 53:429, 1983
Haas GS, Halperin E, Doseretz D, Linggood R, Russel PS, Colvin R, Barrett L, Cosimi AB: Differential recovery of circulating T cell subsets after nodal irradiation for Hodgkin's disease. J Immunol 132:1026, 1984
Foon KA, Schroff RW, Gale RP: Surface markers on leukemia and lymphoma cells: recent advances. Blood 60:1, 1982
Sutherland R, Delia D, Schneider C, Newman R, Kemshead J, Greaves MF: Ubiquitous cell surface glycoprotein on tumors cells is proliferation-associated receptor transferrin. Proc Nat Acad Sci USA 78:4515, 1981
Janossy G, Tidman N, Papageorgiou ES, Kung PC, Goldstein G: Distribution of T lymphocyte subsets in the human bone marrow and thymus: an analysis with monoclonal antibodies. J Immunol 126:1608, 1981
Crawford DH, Francis GE, Wing MA, Edwards AJ, Janossy G, Hoffbrand AV, Prentice HG, Secher D, McConnel J, Kung PC, Goldstein G: Reactivity of monoclonal antibodies with human myeloid precursor cells. Br J Haematol 49:209, 1981
Sieff C, Bicknell D, Caine G, Robinson J, Lam G, Greaves MF: Changes in cell surface antigen expression during hemopoietic differentiation. Blood 60:703, 1982
DeWaele M, Thielemans C, Van Camp BKG: Characterization of immunoregulatory T cells in EBV-indiced infectious mononucleosis by monoclonal antibodies. N Engl J Med 304:460, 1981
Reinherz EL, Cooper MD, Schlossman SF, Rosen FS: Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders. J Clin Invest 68:699, 1981
Terhorst C, van Agthoven A, LeClair K, Snow P, Reinherz EL, Schlossman SF: Biochemical studies of the human thymocyte cell-surface antigens T6, T9 and T10. Cell 23:771, 1981
Matamoros N, Abad E, Webster ADB: LDH isoenzymes in the T lymphocytes of patients with primary hypogammaglobulinaemia. Clin Exp Immunol 50:617, 1982
Reinherz EL, Rosen FS: New concepts of immunodeficiency. Am J Med 71:511, 1981
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Gerli, R., Rambotti, P., Cernetti, C. et al. A mature thymocyte-like phenotypic pattern on human cord circulating T-lymphoid cells. J Clin Immunol 4, 461–468 (1984). https://doi.org/10.1007/BF00916576
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DOI: https://doi.org/10.1007/BF00916576