Skip to main content
SpringerLink
Log in

Wound tissue respiratory burst and local microbial inflammation

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Local and systemic chemiluminescent (CL) response was measured in 32 patients with wound infection and in 27 patients with crush syndrome complicated by severe wound infection. The level of wound CL depends upon the local microbial concentration and the dynamics of wound healing. CL response of neutrophils in circulation was higher in higher local CL response, but extremely high wound microbial concentration (> 108 CFU/g) suppressed both local and systemic responses. No metabolic reserve was observed in wound tissue. Wound healing was characterized by changes in the mode of local CL response: from a prevalence of luminol-dependent (neutrophilic) CL to a prevalence of lucigenin-independent (macrophagal) CL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bordet, J. 1897. Infection streptococcique.Ann. Inst. Pasteur 11:177–213.

    Google Scholar 

  2. Krizek, T. J., andM. C. Robson. 1975. Evolution of quantitative bacteriology in wound management.Am. J. Surg. 130:579–584.

    Google Scholar 

  3. Agarwal, D. S. 1967. Subcutaneous staphylococcal infection in mice. II. The inflammatory response to different strains of staphylococci and micrococci.Br. J. Exp. Pathol. 48:469–482.

    Google Scholar 

  4. Burke, J. F., andA. A. Miles. 1958. The sequence of vascular events in early infective inflammation.J. Pathol. Bacteriol. 76:1–19.

    Google Scholar 

  5. Hart, P. H., L. K. Spencer, M. F. Nulsen, P. J. McDonald, andJ. J. Finlay-Jones. 1986. Neutrophil activity in abscess-bearing mice: comparative studies with neutrophils isolated from peripheral blood, elicited peritoneal exudates, and abscesses.Infect. Immun. 51:936–941.

    Google Scholar 

  6. Zimmerli, W., P. D. Lew, andF. A. Waldvogel. 1984. Pathogenesis of foreign body infection. Evidence for a local granulocyte defect.J. Clin. Invest. 73:1191–1200.

    Google Scholar 

  7. Belotsky, S. M., E. S. Dikovskaya, andV. G. Petukhov. 1987. Luminoldependent chemiluminescence of primary focus against different infective doses ofStaphylococcus aureus.Zurn. Microbiol. Epidemiol. Immunobiol. (Moscow),3:53–55.

    Google Scholar 

  8. Ryan, U. S. 1987. Endothelial cell activation responses.In Pulmonary Endothelium in Health and Disease. U. S. Ryan, editor. Dekker, New York. 3–33.

    Google Scholar 

  9. Shah, S. V., W. H. Baricos, andA. Basci. 1987. Degradation of human glomerular basement membrane by stimulated neutrophils. Activation of a metalloproteinase(s) by reactive oxygen metabolites.J. Clin. Invest. 79:25–31.

    Google Scholar 

  10. Vandenbroucke-Grauls, C. M. J. E., H. M. W. M. Thussen, K. P. M. van Kessel, B. S. van Asbeck, andJ. Verhoef. 1987. Injury to endothelial cells by phagocytosing polymorphonuclear leukocytes and modulatory role of lipoxygenase products.Infect. Immun. 55:1447–1454.

    Google Scholar 

  11. Vissers, M. C. M., W. A. Day, andC. C. Winterburn. 1985. Neutrophils adherent to a nonphagocytosable surface (glomerular basement membrane) produce oxidants only at the site of attachment.Blood 66:161–166.

    Google Scholar 

  12. Fantone, J. C., andP. A. Ward. 1985. Polymorphonuclear leukocyte-mediated cell and tissue injury. Oxygen metabolites and their relations to human disease.Hum. Pathol. 16:973–978.

    Google Scholar 

  13. Briheim, G., P. Follin, S. Sandstedt, andC. Dahlgren. 1989. Relationship between intracellularly and extracellularly generated oxygen metabolites from primed polymorphonuclear leukocytes differs from that obtained from nonprimed cells.Inflammation 13:455–464.

    Google Scholar 

  14. Belotsky, S. M., T. I. Snastina, O. B. Filjukova, andA. A. Zvyagin. 1988. Microbial factor in the chemiluminescence of peripheral blood neutrophils of patients with purulent surgical infection.Zurn. Microbiol. Epidemiol. Immunobiol. (Moscow).8:87–90.

    Google Scholar 

  15. Cohn, Z. 1962. Determination of infection in the peritoneal cavity. I. Response to and fate ofStaphylococcus aureus andStaphlococcus albus in the mouse.Yale J. Biol. Med. 35:12–28.

    Google Scholar 

  16. Kopaniak, M. M., andH. Z. Movat. 1983. Kinetics of acute inflammation induced byEscherichia coli in rabbits. II. The effect of hyperimmunization, complement depletion, and depletion of leukocytes.Am. J. Pathol. 110:13–29.

    Google Scholar 

  17. Skau, T., P.-O. Nystrom, L. Ohman, andO. Stendahl. 1986. The kinetics of peritoneal clearance ofEscherichia coli andBacteroides fragilis and participating defence mechanisms.Arch. Surg. 121:1033–1039.

    Google Scholar 

  18. Devalon, M. L., G. R. Elliott, andW. E. Regelmann. 1987. Oxidative response of human neutrophils, monocytes, and alveolar macrophages induced by unopsonized surface-adherentStaphylococcus aureus.Infect. Immun. 55:2398–2403.

    Google Scholar 

  19. Cheadle, W. G., M. J. Hershman, B. Mays, L. Melton, andH. C. Polk. 1989. Enhancement of survival from murine polymicrobial peritonitis with increase abdominal abscess formation.J. Surg. Res. 47:120–123.

    Google Scholar 

  20. Hansson, L., A. Alwmark, P. Christensen, B. Jeppsson, E. Holst, andS. Bengmark. 1985. Standardized intraabdominal abscess formation with generalized sepsis: Pathophysiology in the rat.Eur. Surg. Res. 17:155–159.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. A. V. Vishnevsky Institute of Surgery, Moscow, USSR

    Sandro M. Belotsky, Eugenij V. Guzu, Vladimir A. Karlov, Eugenia S. Dikovskaya, Olga B. Filjukova & Tatiana I. Snastina

Authors
  1. Sandro M. Belotsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eugenij V. Guzu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vladimir A. Karlov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eugenia S. Dikovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Olga B. Filjukova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tatiana I. Snastina
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dr. Tatiana Snastina was a “major scientist” in the Institute of Surgery when this study was done.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Belotsky, S.M., Guzu, E.V., Karlov, V.A. et al. Wound tissue respiratory burst and local microbial inflammation. Inflammation 14, 663–668 (1990). https://doi.org/10.1007/BF00916369

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00916369

Keywords

Search

Navigation