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The transfer of antigen-specific humoral immunity from marrow donors to marrow recipients

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Abstract

This study shows that specific humoral immunity could be transferred from marrow donors to marrow recipients. Peripheral blood lymphocytes from long-term human marrow recipients produced IgG anti-tetanus toxoid antibody afterin vitro tetanus toxoid stimulation. Antitetanus toxoid antibody biosynthesis was induced using a new tetanus toxoid-specific system employing high lymphocyte numbers, many replicate microcultures, and antigen washout. Anti-tetanus toxoid antibody in 12-day culture supernatants was detected using an enzyme-linked immunosorbent assay. Of 14 marrow recipients, 6 (5 with and 1 without chronic graft-vs-host disease) had lymphocytes that produced anti-tetanus toxoid antibody. Culturing additional numbers of marrow recipient lymphocytes increasedin vitro biosynthesis of anti-tetanus toxoid antibody. The presence of circulating serum antibodies to tetanus toxoid and the production of specific anti-tetanus toxoid antibody by peripheral blood lymphocytes from marrow recipients show that engrafted donor lymphocytes can producein vitro specific antibodies to recall antigens without postgrafting reimmunization.

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References

  1. Witherspoon RP, Lum LG, Storb R: Immunologic reconstitution after human marrow grafting. Semin Hematol 21:2–10, 1984

    Google Scholar 

  2. Lum LG, Storb R: Bone marrow transplantation.In Principles of Organ Transplantation, MW Flye (ed). New York, WB Saunders, 1985, Chap 19

    Google Scholar 

  3. Neiman P, Wasserman PB, Wentworth BB, Kao GF, Lerner KG, Storb R, Buckner CD, Clift RA, Fefer A, Fass L, Glucksberg H, Thomas ED: Interstitial pneumonia and cytomegalovirus infection as complications of human marrow transplantation. Transplantation 15:478–485, 1973

    Google Scholar 

  4. Winston DJ, Schiffman G, Wang DC, Feig SA, Lin C-H, Marso EL, Ho WG, Young LS, Gale RP: Pneumococcal infections after human bone-marrow transplantation. Ann Intern Med 91:835–841, 1979

    Google Scholar 

  5. Atkinson K, Storb R, Prentice RP, Weiden PL, Witherspoon RP, Sullivan K, Noel D, Thomas ED: Analysis of late infections in 89 long-term survivors of bone marrow transplantation. Blood 53:720–731, 1979

    Google Scholar 

  6. Friedrich W, O'Reilly RJ, Koziner B, Gebhard DF Jr., Good RA, Evans RL: T-lymphocyte reconstitution in recipients of bone marrow transplants with and without GVHD: Imbalances of T-cell subpopulations having regulatory and cognitive functions. Blood 59:696–701, 1982

    Google Scholar 

  7. De Bruin HG, Astaldi A, Leupers T, van de Griend RJ, Dooren LJ, Schellekens PTA, Tanke HJ, Roos M, Voosen JM: T lymphocyte characteristics in bone marrow-transplanted patients. II. Analysis with monoclonal antibodies. J. Immunol 127:244–251, 1981

    Google Scholar 

  8. Atkinson K, Hansen JA, Storb R, Goehle S, Goldstein G, Thomas ED: T-cell subpopulations identified by monoclonal antibodies after human marrow transplantation. I. Helper-inducer and cytotoxic-suppressor subsets. Blood 59:1292–1298, 1982

    Google Scholar 

  9. Forman SJ, Nocker P, Gallagher M, Zaia J, Wright C, Bolen J, Mills B, Hecht T, Blume K: Pattern of T cell reconstitution following allogeneic bone marrow transplantation for acute hematological malignancy. Transplantation 34:96–98, 1982

    Google Scholar 

  10. Lum LG, Seigneuret MC, Storb RF, Witherspoon RP, Thomas ED: In vitro regulation of immunoglobulin synthesis after marrow transplantation. I. T-cell and B-cell deficiencies in patients with and without chronic graft-versus-host disease. Blood 58:431–439, 1981

    Google Scholar 

  11. Korsmeyer SJ, Elfenbein GJ, Goldman CK, Marshall SL, Waldmann TA: B cell, helper T cell and suppressor T cell abnormalities contribute to disordered immunoglobulin synthesis in patients following bone marrow transplantation. Transplantation 33:184–190, 1982

    Google Scholar 

  12. Lum LG, Seigneuret MC, Orcutt-Thordarson N, Ostenson RC, Storb R: Immunoglobulin production after marrow transplantation. III. The functional heterogeneity of Fc-IgG receptor positive and negative T cell subpopulations. Clin Exp Immunol 48:675–684, 1982

    Google Scholar 

  13. Lum LG, Seigneuret MC, Orcutt-Thordarson N, Storb R: Immunoglobulin production after marrow transplantation. IV. T4 and T8 subset function in patients with chronic graft-versus-host disease. J Immunol 129:113–119, 1982

    Google Scholar 

  14. Pahwa SG, Pahwa RN, Friedrich W, O'Reilly RJ, Good RA: Abnormal humoral immune responses in peripheral blood lymphocyte cultures of bone marrow transplant recipients. Proc Natl Acad Sci (USA) 79:2663–2667, 1982

    Google Scholar 

  15. Lum LG, Seigneuret MC, Orcutt-Thordarson N, Noges JE, Storb R: The regulation of immunoglobulin synthesis after HLA-identical bone marrow transplantation: VI. Differential rates of maturation of distinct functional groups within lymphoid subpopulations in patients after human marrow grafting. Blood 65:1422–1433, 1985

    Google Scholar 

  16. Stevens RH, Saxon A: Immunoregulation in humans. Control of antitetanus toxoid antibody production after booster immunization. J Clin Invest 62:1154–1160, 1978

    Google Scholar 

  17. Volkman DJ, Allyn SP, Fauci AS: Antigen-induced antibody production in humans: Tetanus toxoid-specific antibody synthesis. J Immunol 129:107–111, 1982

    Google Scholar 

  18. Yarchoan R, Murphy BR, Strober W, Clements ML, Nelson DL: In vitro production of anti-influenza virus antibody after intranasal inoculation with cold-adapted influenza virus. J Immunol 127:1958–1963, 1981

    Google Scholar 

  19. Kishimoto S, Tomino S, Mitsuya H, Fujiwara H, Tsuda H: Age-related decline in thein vitro andin vivo syntheses of anti-tetanus toxoid antibody in humans. J Immunol 125:2347–2352, 1980

    Google Scholar 

  20. Lum LG, Culbertson NJ: The induction and suppression ofin vitro IgG anti-tetanus toxoid antibody synthesis by human lymphocytes stimulated with tetanus toxoid in the absence ofin vivo booster immunizations. J Immunol 135:185–191, 1985

    Google Scholar 

  21. Thomas ED, Buckner CD, Banaji M, Clift RA, Fefer A, Flournoy N, Goodell BW, Hickman RO, Lerner PE, Neiman GE, Sale JE, Sanders J, Singer J, Stevens M, Storb R, Weiden PL: One hundred patients with acute leukemia treated by chemotherapy, total body irradiation, and allogeneic marrow transplantation. Blood 49:511–533, 1977

    Google Scholar 

  22. Thomas ED, Clift RA, Hersman J, Sanders J, Stewart P, Buckner CD, Fefer A, McGuffin R, Smith JW, Storb R: Marrow transplantation for acute nonlymphoblastic leukemia in first remission using fractionated or single-dose irradiation. Int J Radiat Oncol Biol Phys 8:817–821, 1982

    Google Scholar 

  23. Clift RA, Buckner CE, Thomas ED, Sanders JE, Stewart PS, Sullivan KM, McGuffin R, Hersman J, Sale GE, Storb R: Allogeneic marrow transplantation using fractionated total body irradiation in patients with acute lymphoblastic leukemia in relapse. Leuk Res 6:401–407, 1982

    Google Scholar 

  24. Storb R, Thomas ED, Buckner CD, Clift RA, Johnson FL, Fefer A, Glucksberg H, Giblett ER, Lerner KG, Neiman P: Allogeneic marrow grafting for treatment of aplastic anemia. Blood 43:157–180, 1974

    Google Scholar 

  25. Lum LG, Munn NA, Schanfield MS, Storb R: The detection of specific antibody formation to recall antigens after human bone marrow transplantation. Blood 67:582–587, 1986

    Google Scholar 

  26. Lefkovits I: Limiting dilution analysis.In Immunological Methods, I Lefkovits, B Pernis (eds). New York, Academic Press, 1979, pp 355–370

    Google Scholar 

  27. Shiobara S, Lum LG, Witherspoon RP, Storb R: Antigen specific antibody responses of lymphocytes to tetanus toxoid after human marrow transplantation. Transplantation 41:587–592, 1986

    Google Scholar 

  28. Tsoi MS, Storb R, Dobbs S, Kopecky KJ, Santos E, Weiden PI, Thomas ED: Nonspecific suppressor cells in patients with chronic graft-vs-host disease after marrow grafting. J Immunol 123:1970–1976, 1979

    Google Scholar 

  29. Tsoi MS, Storb R, Dobbs S, Thomas ED: Specific suppressor cells in graft-host tolerance of HLA-identical marrow transplantation. Nature 292:355–357, 1981

    Google Scholar 

  30. Shiobara S, Witherspoon RP, Lum LG, Storb R: Immunoglobulin synthesis after HLA-identical marrow grafting: V. The role of peripheral blood monocytes in the regulation ofin vitro immunoglobulin secretion stimulated by PW mitogen. J Immunol 132:2850–2856, 1984

    Google Scholar 

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Authors and Affiliations

  1. Bone Marrow Transplant Program, Medical College of Wisconsin, 53226, Milwaukee, Wisconsin

    Lawrence G. Lum

  2. Fred Hutchinson Cancer Research Center and Division of Oncology, Departments of Pediatrics and Medicine, University of Washington, School of Medicine, 98104, Seattle, Washington

    Lawrence G. Lum, Margaret C. Seigneuret & Rainer Storb

Authors
  1. Lawrence G. Lum
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  2. Margaret C. Seigneuret
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  3. Rainer Storb
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Lum, L.G., Seigneuret, M.C. & Storb, R. The transfer of antigen-specific humoral immunity from marrow donors to marrow recipients. J Clin Immunol 6, 389–396 (1986). https://doi.org/10.1007/BF00915378

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  • DOI: https://doi.org/10.1007/BF00915378

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