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Pulmonary inflammation and fibrosis in a murine model of asbestosis and silicosis

Possible role of tumor necrosis factor

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Abstract

Prolonged asbestos and silica inhalation is associated with pulmonary inflammation and fibrosis. Several studies suggest that TNF may play a rolc in the development of inflammation and fibrosis. We studied TNF production in a murine model of asbestosis and silicosis. Asbestos fibers caused a significant inflammatory response at two weeks and pulmonary fibrosis beginning at one month. Pulmonary inflammation was principally caused by an accumulation of neutrophils (0.88 × 105 neutrophils/compared to 0.05×105 in controls). TNF production by bronchoalveolar cells was higher in asbestos-instilled mice at two weeks, but was significantly diminished in older mice. Pulmonary inflammation was observed until six months in silica-instilled mice. Neutrophils were also the principal protagonists of the inflammation. In this group, severe fibrosis was observed at two weeks. TNF production in silica-instilled mice was similar to controis, possibly due to the presence of large numbers of neutrophils (3.3 × 105/lavage) that could adsorb TNF. In vitro experiments showed an augmentation of TNF production by bronchoalveolar cells in the presence of silica. Taken together, our data suggest that asbestos and silica stimulate alveolar macrophages to produce TNF, which can be involved in pulmonary inflammation and fibrosis.

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Authors and Affiliations

  1. Immunology Division, Faculty of Medicine, University of Sherbrooke, J1H 5N4, Sherbrooke, Québec, Canada

    Elyse Bissonnette & Marek Rola-Pleszczynski

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  1. Elyse Bissonnette
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  2. Marek Rola-Pleszczynski
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Additional information

This work was supported by a grant from the Medical Research Council of Canada.

E. B. is a recipient of a Studentship from the Fonds de la Recherche en Santé du Québec.

M. R.-P. is a Scholar of the Fonds de la Recherche en Santé du Québec.

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Bissonnette, E., Rola-Pleszczynski, M. Pulmonary inflammation and fibrosis in a murine model of asbestosis and silicosis. Inflammation 13, 329–339 (1989). https://doi.org/10.1007/BF00914399

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