Abstract
The utility of thermitase, a thermophilic proteolytic enzyme fromThermoactinomyces vulgaris, as a catalyst for peptide synthesis has been studied using several Nα-benzyloxycarbonyl dipeptide esters as carboxyl components and Leu-NH2 as nucleophile. Couplings were most successful with substrates containing Met, Leu, Ala, Phe, Tyr in P1-position, and Val, Ala, Pro in P2, whereas the substrates with Val, Ile, Pro in P1 failed to couple.
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Könnecke, A., Jakubke, HD. Versuche zur Anwendung von Thermitase als Katalysator zur Knüpfung der Peptidbindung. Monatshefte für Chemie 112, 1099–1102 (1981). https://doi.org/10.1007/BF00905078
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DOI: https://doi.org/10.1007/BF00905078