Abstract
Two strains ofLeptospirillum-like bacteria, L6 and L8, have been isolated from a mixed inoculum, also containingThiobacillus ferrooxidans andT. thiooxidans, cultured for one year with a colbaltiferous pyrite as energy substrate in a 100 I continuous bioleaching laboratory unit. Several physiological properties of the strains are described. The vibrio-shaped microorganisms grew at pH values lower than 1.3. Their growth rate was maximum between 2.5 and 8.0 g l1 ferrous iron. The optimal growth temperature was 37.5° C. Ferric iron had a stimulative effect on bacterial development up to 8 g l−1, and growth was as rapid at 14 g l−1 ferric iron as at 8 g l−1. The negative influence of cobalt on the final cell concentration was observed at 0.5 g l−1, but the growth rate was not affected up to 2 g l−1. The G + C content of strains L8 is 55.6 mol%.
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Battaglia, F., Morin, D., Garcia, JL. et al. Isolation and study of two strains ofLeptospirillum-like bacteria from a natural mixed population cultured on a cobaltiferous pyrite substrate. Antonie van Leeuwenhoek 66, 295–302 (1994). https://doi.org/10.1007/BF00882763
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DOI: https://doi.org/10.1007/BF00882763