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Numerical and chemical classification ofStreptosporangium and some related actinomycetes

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Abstract

One hundred and seventeen streptosporangia from soil were compared with marker strains of the familyStreptosporangiaceae for many phenotypic properties. The data were examined using the Jaccard, pattern and simple matching coefficients with clustering achieved using average, complete and single linkage algorithms. Particular confidence was placed in the product of the pattern, average linkage analysis given the sharp definition of aggregate groups and clusters and a combination of low test error and high cophenetic correlation values. The test strains were assigned to five aggregate groups that were equated with the generaStreptosporangium (group A),Microbispora (group B),Planobispora andPlanomonospora (Group C),Kutzneria (neéStreptosporangium viridogriseum (group D), andMicrotetraspora (group E). The streptosporangia, both isolates and marker strains, were assigned to 5 major, 7 minor and 18 single membered clusters. Representative streptosporangia examined for chemical markers were characterised by the presence ofmeso-diaminopimelic acid in whole-organism hydrolysates, complex mixtures of straight- and branched chain fatty acids, di- and tetrahydrogenated menaquinones as predominant isoprenologues, and complex polar lipid patterns containing diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and uncharacterised components. The chemical and numerical data support the taxonomic integrity of the validly described species ofStreptosporangium and suggest that the genus is markedly underspeciated.

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Authors and Affiliations

  1. Department of Microbiology, The University, NE1 7RU, Newcastle upon Tyne, UK

    Timothy S. Whitham & Michael Goodfellow

  2. Department of Chemistry, The University, NE1 7RU, Newcastle upon Tyne, UK

    David E. Minnikin

  3. Chemotherapeutic Research Centre, SmithKline Beecham Pharmaceuticals, Brockham Park, RH3 7AJ, Betchworth, Surrey, UK

    Timothy S. Whitham & Medha Athalye

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  1. Timothy S. Whitham
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Whitham, T.S., Athalye, M., Minnikin, D.E. et al. Numerical and chemical classification ofStreptosporangium and some related actinomycetes. Antonie van Leeuwenhoek 64, 387–429 (1993). https://doi.org/10.1007/BF00873094

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