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A biphasic approach to the determination of the phenotypic and genotypic diversity of some anaerobic, cellulolytic, thermophilic, rod-shaped bacteria

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Abstract

A biphasic approach, involving a numerical phenetic and a phylogenetic study, was used to determine diversity among some anaerobic, cellulolytic, thermophilic, rod-shaped bacteria. Ninety two characters were determined for 51 strains in the numerical taxonomy study, and partial 16S rDNA sequences from 16 isolates were compared. Both the phenetic and phylogenetic data indicate diversity within this group of organisms, and reveal the lack of similarity between sporogenous and asporogenous isolates. The results of the phylogenetic study demonstrate the lack of relationship of the majority of the strains studied to previously studied thermophilic bacteria. In general, good correlation exists between the two data sets, but discrepancies arise when strains with a high level of similarity are examined. The need for caution in the interpretation of data obtained from such a biphasic approach is discussed.

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References

  • Cato EP & Stackebrandt E (1989) Taxonomy and phylogeny. In: Minton NP & Clarke DJ (Eds) Biotechnology Handbooks 3. Clostridia (pp 1–26). Plenum Press, London

    Google Scholar 

  • Cato EP, George LW & Finegold SM (1984) GenusClostridium Prazmowski. In: Sneath PHA, Mair NS, Sharpe ME & Holt JG (Eds) Bergey's Manual of Systematic Bacteriology, Volume 2 (pp 1141–1200). Williams & Wilkins, Baltimore

    Google Scholar 

  • De Soete G (1983) A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48: 621–626

    Google Scholar 

  • Del Moral A, Prado B, Quesada E, Garcia T, Ferrer R & Ramos-Cormenzana A (1988) Numerical taxonomy of moderately halophilic Gram-negative rods from an inland saltern. J. Gen. Microbiol. 134: 733–741

    Google Scholar 

  • Goodfellow M & O'Donnell AG (1993) Roots of bacterial systematics. In: Goodfellow M & O'Donnell AG (Eds) Handbook of New Bacterial Systematics (pp 3–544). Academic Press, London

    Google Scholar 

  • Gregersen T (1978) Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur. J. Appl. Microbiol. Biotech. 5: 123–127

    Google Scholar 

  • Hudson JA, Morgan HW & Daniel RM (1989) Numerical classification ofThermus isolates from globally distributed hot springs. System. Appl. Microbiol. 11: 250–256.

    Google Scholar 

  • Hudson JA, Morgan HW & Daniel RM (1990a) A survey of cellulolytic anaerobic thermophiles from hot springs. System. Appl. Microbiol. 13: 72–76

    Google Scholar 

  • Hudson JA, Morgan HW & Daniel RM (1990b) Cellulolytic properties of an extremely thermophilic anaerobe. Appl. Microbiol. Biotech. 33: 687–691

    Google Scholar 

  • Jeffries CD, Holtman DF & Guse DG (1957) Rapid method for determining the activity of microorganisms on nucleic acids. J. Bacteriol. 73: 590–591

    Google Scholar 

  • Jin F & Toda K (1988) Isolation of a new anaerobic, thermophilic and cellulolytic bacteria-JT and their cellulase production. J. Ferm. Technol. 66: 389–395

    Google Scholar 

  • Jin F, Yamasato K & Toda K (1988)Clostridium thermocopriae sp. nov., a cellulolytic thermophile from animal feces, compost, soil and a hot spring in Japan. Int. J. System. Bacteriol. 38: 277–281

    Google Scholar 

  • Jones BE & Lindsay JM (1989) An extreme thermophilic, anaerobic eubacterium which hydrolyses cellulose. Proceedings: Academy Workshop on Adaptation of Microorganisms to Extreme Environments, Haren, Netherlands, June 5–7 1989

  • Jukes TH & Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian Protein Metabolism (pp 21–132). Academic Press, New York

    Google Scholar 

  • LeRuyet P, Dubourguier HC, Albagnac G & Prensier G (1985) Characterization ofClostridium thermolacticum sp. nov. a hydrolytic, thermophific anaerobe producing high amounts of lactate. System. Appl. Microbiol. 6: 196–202

    Google Scholar 

  • Madden RH (1983) Isolation and characterization ofClostridium stercorarium sp. nov., a cellulolytic thermophile. Int. J. System. Bacteriol. 33: 837–840

    Google Scholar 

  • Mendez BS, Pettinari MJ, Ivanier SE, Ramos CA & Sineriz F (1991)Clostridium thermopapyrolyticum sp. nov., a cellulolytic thermophile. Int. J. System. Appl. Microbiol. 41: 281–283

    Google Scholar 

  • Ng TK, Weimer PJ & Zeikus JG (1977) Cellulolytic and physiological properties ofClostridium thermocellum. Arch. Microbiol. 114: 1–7

    Google Scholar 

  • Olsen GJ & Woese CR (1993) Ribosomal RNA: a key to phylogeny. FASEB J. 7: 113–123

    Google Scholar 

  • Patel BKC, Monk C, Littleworth H, Morgan HW & Daniel RM (1987)Clostridium fervidus sp. nov., a chemoorganotrophic acetogenic thermophile. Int. J. System. Appl. Microbiol. 37: 123–136

    Google Scholar 

  • Patel BKC, Morgan HW & Daniel RM (1985)Fervidobacterium nodosum gen. nov. and spec. nov., a new chemoorganotrophic, caldoactive, anacrobic bacterium. Arch. Microbiol. 141: 63–69

    Google Scholar 

  • Priest FG, Goodfellow M & Todd C (1988) A numerical classification of the genusBacillus. J. Gen. Microbiol. 134: 1847–1882

    Google Scholar 

  • Priest FG & Pleasants JG (1988) Numerical taxonomy of some leuconostoes and related bacteria from Scotch whisky distilleries. J. Appl. Bacteriol. 64: 379–387

    Google Scholar 

  • Rainey FA, Dorsch M, Morgan HW & Stackebrandt E (1992) 16S rDNA analysis ofSpirochaeta thermophila: Its phylogenetic position and implications for the systematics of the OrderSpirochaetales. System. Appl. Microbiol. 15: 197–202

    Google Scholar 

  • Rainey FA, Ward NL, Morgan HW, Toalster R & Stackebrandt E (1993) A phylogenetic analysis of anaerobic thermophilic bacteria: aid for their reclassification. J. Bacteriol. 175: 4772–4779

    Google Scholar 

  • Sneath PHA & Sokal RR (1973) Numerical Taxonomy: The Principles and Practice of Numerical Classification. W.H. Freeman, San Francisco

    Google Scholar 

  • Sneath PHA (1957a) Some thoughts on bacterial classification. J. Gen. Microbiol. 17: 184–200

    Google Scholar 

  • Sneath PHA (1957b) The application of computers to taxonomy. J. Gen. Microbiol. 17: 201–226

    Google Scholar 

  • Sokal RR & Michener CD (1958) A statistical method for evaluating systematic relationships. Univ. Kan. Sci. Bull. 38: 1409–1438

    Google Scholar 

  • Sokal RR (1985) The principles of numerical taxonomy: twenty-five years later, p. 1–14. In: Goodfellow M, Jones D & Priest FG (Eds) Computer-assisted Bacterial Systematics (pp 1–20). Academic Press, London

    Google Scholar 

  • Stackebrandt E (1991) Unifying phylogeny and phenotypic diversity. In: Balows A, Trüper HG, Dworkin M, Harder W & Schleifer K-H (Eds) The Prokaryotes, 2nd Edition (pp 19–47). Springer-Verlag, New York

    Google Scholar 

  • Stackebrandt E & Goodfellow M (Eds) (1991) Nucleic Acid Techniques in Bacterial Systematics. John Wiley & Sons, Chichester

    Google Scholar 

  • Svetlichny V, Aksenova HYu & Zavarzin G (1990) Extremely thermophilic cellulolytic anaerobes from hot springs of the Soviet Far East. Poster Abstract. Thermophily Today. International Meeting on Cell Constituents, Genetics and Biotechnological Applications of Thermophilic Microorganisms. Viterbo, Italy. May 7–9, 1990

  • Williams AG (1983) Staining reactions for the detection of hemicellulose-degrading bacteria. FEMS Microbiol. Letts. 20: 253–258

    Google Scholar 

  • Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA & Sackin MJ (1983) Numerical classification ofStreptomyces and related genera. J. Gen. Microbiol. 129: 1743–1813

    Google Scholar 

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Author notes

  1. Peter H. Janssen

    Present address: MPI für Terrestrische Mikrobiologie Karl-von-Frisch-Strasse, D-35043, Marburg, Germany

Authors and Affiliations

  1. Centre for Microbial Identification and Diversity, Department of Microbiology, The University of Queensland, Brisbane, Australia

    Frederick A. Rainey & Erko Stackebrandt

  2. Department of Biological Science, The University of Waikato, Hamilton, New Zealand

    Frederick A. Rainey, Peter H. Janssen & Hugh W. Morgan

  3. DSM Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1B, 38124, Braunschweig, Germany

    Frederick A. Rainey & Erko Stackebrandt

Authors
  1. Frederick A. Rainey
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  2. Peter H. Janssen
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  3. Hugh W. Morgan
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  4. Erko Stackebrandt
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Rainey, F.A., Janssen, P.H., Morgan, H.W. et al. A biphasic approach to the determination of the phenotypic and genotypic diversity of some anaerobic, cellulolytic, thermophilic, rod-shaped bacteria. Antonie van Leeuwenhoek 64, 341–355 (1993). https://doi.org/10.1007/BF00873092

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  • DOI: https://doi.org/10.1007/BF00873092

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