Summary
TheDrosophila chorion contains an endogenous peroxidase activity which remains inactive until late stage 14 when it catalyzes the crosslinking of the chorionic proteins. Using explanted follicles developing in vitro, premature, but otherwise normal crosslinking can be induced with hydrogen peroxide and normal crosslinking can be prevented with peroxidase inhibitors. Inhibition or premature activation of the shell peroxidase allows characterization of chorionic filament specific proteins and establishes new criteria for the identification of eggshell components.
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References
Anderson SO (1966) Covalent crosslinks in a structural protein, resilin. Acta Physiol Scand 66 Suppl 263:1–81
DeVore DP, Gruebel RJ (1977) Identification of dityrosine in adhesive formed by the sea mussel,Mytilus edulis. Fed Proc 36:679
Ephrussi B, Beadle GW (1936) A technique of transplantation for Drosophila. Am Nat 70:218–225
Fahimi HD (1970) The fine structural localization of endogenous and exogenous peroxidase activity in Kupffer cells of rat liver. J Cell Biol 47:247–262
Foerder CA, Shapiro BM (1977) Release of ovoperoxidase from sea urchin eggs hardens the fertilization membrane with tyrosine crosslinks. Proc Natl Acad Sci USA 74:4214–4218
Giorgi F, Deri P (1976) Cytochemistry of late ovarian chambers ofDrosophila melanogaster. Histochemistry 48:325–334
Hall G (1978) Hardening of the sea urchin fertilization envelope by peroxidase-catalyzed phenolic coupling of tyrosines. Cell 15:343–355
Kawasaki H, Sata H, Suzuki M (1974) Structural proteins in the egg envelopes of dragonflies,Sympetrum infuscatum andS. frequens. Insect Biochem 4:99–111
Keeley FW, LaBella FS (1972) Isolation of dityrosine from an alkaline-soluble connective tissue protein. Biochim Biophys Acta 263:52–59
King RC (1970) Ovarian development inDrosophila melanogaster. Academic Press, New York
LaBella F, Keeley F, Vivian S, Thornhill D (1967) Evidence of dityrosine in elastin. Biochem Biophys Res Commun 26:748–753
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriphage T4. Nature 227:680–685
Laskey RA, Mills AD (1975) Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur J Biochem 56:335–341
Locke M (1969) The localization of a peroxidase associated with hard cuticle formation in an insect,Calpodes ethlius Stroll, Lepidoptera, Hesperiidae. Tissue Cell 1:555–574
Margaritis LH, Petri WH, Kafatos FC (1976) Three dimensional structure of the endochorion in wild-typeDrosophila melanogaster. J Exp Zool 198:403–408
Margaritis LH, Kafatos FC, Petri WH (1980) The eggshell ofDrosophila melanogaster: I. The fine structure of the layers and regions of the wild-type eggshell. J Cell Sci 43:1–35
Petri WH, Wyman AR, Kafatos FC (1976) Specific protein synthesis in cellular differentiation III. The eggshell proteins ofDrosophila melanogaster and their program of synthesis. Dev Biol 49:185–199
Petri WH, Mindrinos MN, Lombard MF, Margaritis LH (1979) In vitro development of theDrosophila chorion in a chemically defined organ culture medium. Wihelm Roux's Arch 186:351–362
Petri WH, Mindrinos MN, Lombard MF (1979a) Independence of vitelline membrane and chorion crosslinking in theDrosophila melanogaster eggshell. J Cell Biol 83:23a
Raven DJ, Earland C, Little M (1971) Occurrence of dityrosine in Tussah silk fibroin and keratin. Biochim Biophys Acta 251:96–99
Robb JA (1969) Maintenance of imaginal discs ofDrosophila melanogaster in chemically defined media. J Cell Biol 41:876–885
Roels F (1971) Peroxidatic activity inArtemia salina oocytes: role in egg-envelope hardening. Exp Cell Res 69:452–456
Waring GL, Mahowald AP (1979) Identification and time of synthesis of chorion proteins in Drosophila melanogaster. Cell 16:599–607
Worthington Biochemical Corporation (1972) Worthington Enzyme Manual. Freehold, New Jersey
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Mindrinos, M.N., Petri, W.H., Galanopoulos, V.K. et al. Crosslinking of theDrosophila chorion involves a peroxidase. Wilhelm Roux' Archiv 189, 187–196 (1980). https://doi.org/10.1007/BF00868677
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DOI: https://doi.org/10.1007/BF00868677