Summary
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1.
Umbrellar fragments of the leptomedusaCampanularia johnstoni with or without parts of the radial canal demonstrate a gradient in the potential for manubrium regeneration and in regeneration time.
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2.
Implantation experiments exclude the manubrium as a source of inhibition or induction in the regeneration of another manubrium. One special case of inhibition appears to be due to competition for a common substrate.
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3.
Medusa fragments consisting of only peripheral umbrella (C-fragments) undergo a considerably different restitution process as compared with fragments including a central portion of the umbrella (A-fragments). Vital stain is seen to disperse in the subumbrellar tissues during this process in C-fragments, whereas vital stain in A-fragments is observed to accumulate and later on is incorporated into the regenerating manubrium.
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4.
The mesogloea of different-sized A-fragments retains a stable form when freed of its adhering cellular components, after a 12–24 h regeneration period; for C-fragments, however, the same result is not observed until 72 to 96 h after their excision.
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5.
InPodocoryne carnea the observed gradients in manubrium regeneration can be abolished when the subumbrellar tissues are separated from the mesogloea by collagenase treatment.
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6.
A model for manubrium regeneration in interradial fragments, based on the influence of tension exerted by the cicatrization process and the counteracting mesogloeal force, is presented and discussed.
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References
Burnett, A. L.: The acquisition, maintenance, and lability of the Differentiated State inHydra. In: The stability of the differentiated state (ed.: H. Ursprung), p. 109–127. Berlin-Heidelberg-New York: Springer 1968
Gladfelder, W. B.: A comparative analysis of the locomotory systems of medusoid cnidaria. Helgoländer Wiss. Meeresunters.25, 228–272 (1973)
Humphreys, T.: Chemical dissolution and in vitro reconstruction of sponge cell adhesions. Develop. Biol.8, 27–47 (1963)
Hyman, L. H.: The Invertebrate. I. McGraw-Hill Book Company, Inc. 1951
Marcel, R.: Effects inhibiteur et trophique dans la régénération céphalique deEisenia foetida. Annales d'Embryol. et de Morphogén. 1,4, 417–425 (1968)
Rose, M. S.: The correlation between bioelectrical and morphogenetic polarity during regeneration inTubularia. Develop. Biol.28, 274–279 (1972)
Schaller, H. Ch.: Isolation and characterization of a low-molecular-weight substance activating head and bud formation inHydra. J. Embryol. exp. Morph. Vol. 29,1, 27–38 (1973)
Schmid, B.: Die Mesogloa der LeptomedusePhialidium hemisphaericum. Thesis, unpublished, Zool. Institut University of Zurich 1975
Schmid, B., Schmid, V., Tardent, P.: The umbrellar growth process in the leptomedusaPhialidium hemisphaericum. Exp.30, 1399 (1974)
Schmid, V.: Untersuchungen über Dedifferenzierungsvorgänge bei Medusenknospen und Medusen vonPodocoryne carnea M.. Sars. Wilhelm Roux' Archiv169, 281–307 (1972)
Schmid, V.: Regeneration in medusa buds and medusae of hydrozoa. Amer. Zool.14, 773–781 (1974a)
Schmid, V.: Structural alterations in cultivated striated muscle cells from anthomedusae (Hydrozoa). Exptl. Cell Res.86, 193–198 (1974b)
Schmid, V.: Cell transformation in isolated striated muscle of hydromedusae independent of DNA-synthesis. Exp. Cell Res.94, 401–408 (1975a)
Schmid, V.: The transformational potential of striated muscle in Hydromedusae. Develop. Biol., in press (1975b)
Schmid, V., Tardent, P.: Zur Gametogenese vonPodocoryne carnea M. Sars. R. suisse Zool.76, 1071–1078 (1969)
Schmid, V., Tardent, P.: The reconstitutional performances of the leptomedusaCampanularia johnstoni. Marine Biology8, 99–104 (1971)
Schneider, B.: Die Manubriumregeneration bei der LeptomedusePhialidium hemisphaericum: Einfluß der Ernährung und der Gewebespannung. Thesis, unpublished, Zool. Institut, University of Zurich 1975
Stidwill, R.: Inhibitions- und Inductionsphaenomene bei der Manubriumregeneration der LeptomedusePhialidium hemisphaericum. Thesis, unpublished, Zool. Institut, University of Zurich 1975
Singer, M., Rzehak, K., Maier, C. C.: The relation between the caliber of the axon and the trophic activity of nerves in limb regeneration. J. Exptl. Zool.166, 89–98 (1967)
Stockdale, F. E., Abbott, J., Holtzer, S., Holtzer, H.: The loss of phenotypic traits by differentiated cells. Develop. Biol.7, 293–302 (1963)
Tardent, P.: Regeneration in Hydrozoa. Biol. Rev.38, 293–333 (1963)
Webster, G.: Morphogenesis and pattern formation in hydroids. Biol. Rev.46 1–46 (1971)
Wolpert, L.: Positional information and the spatial pattern of cellular differentiation. J. theor. Biol.25, 1–47 (1969)
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Schmid, V., Schmid, B., Schneider, B. et al. Factors effecting manubrium-regeneration in hydromedusae (Coelenterata). Wilhelm Roux' Archiv 179, 41–56 (1976). https://doi.org/10.1007/BF00857639
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DOI: https://doi.org/10.1007/BF00857639