Abstract
The formation of the metanephric kidney is dependent upon the timed and sequential expression of a number of polypeptide growth factors. To shed light on the participation of members of the insulin-like growth factor (IGF) and epidermal growth factor/transforming growth factor-α (EGF/TGF-α) families, we measured the synthesis of IGF-I, IGF-II, EGF and TGF-α by developing rat metanephroi in organ culture and determined the effect of anti-growth factor antibodies on growth and development. IGF-I, IGF-II and TGF-α were produced by metanephroi and released into culture media. We could detect no EGF. Inclusion of anti-IGF-I, anti-IGF-II, anti-IGF-II receptor or anti-TGF-α antibodies in organ cultures inhibited growth and development of metanephroi. Our findings suggest that both members of the IGF family and TGF-α are produced within the developing metanephros and promote renal organogenesis.
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Hammerman, M.R., Rogers, S.A. & Ryan, G. Growth factors and kidney development. Pediatr Nephrol 7, 616–620 (1993). https://doi.org/10.1007/BF00852568
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DOI: https://doi.org/10.1007/BF00852568