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The development of anti-glomerular basement membrane nephritis in two children with Alport's syndrome after renal transplantation: characterization of the antibody target

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Abstract

Two children with Alport's syndrome are described, who developed anti-glomerular basement membrane (GBM) antibody-mediated nephritis after renal transplantation. The reactivity of antibodies in their serum with collagenase-solubilized normal GBM was examined by SDS-PAGE with one- and two-dimensional immunoblotting. The specificity was compared with that of antibodies present in serum from a patient with Goodpasture's syndrome, and a mouse monoclonal antibody (MCA-P1), directed against the Goodpasture antigen. All reacted in a similar way with collagenase-solubilized GBM. Since abnormalities in the composition of the GBM are present in Alport's syndrome, it is proposed that differing antigen composition of GBM in the host compared with the donor kidney, together with transplant rejection, may have provoked the development of post-transplant anti-GBM antibodies.

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Authors and Affiliations

  1. Department of Biochemistry, University of Nijmegen, P. O. B. 9101, NL-6500 HB, Nijmegen, The Netherlands

    L. P. W. J. v. d. Heuvel & J. H. Veerkamp

  2. Department of Paediatrics, University of Nijmegen, P. O. B. 9101, NL-6500 HB, Nijmegen, The Netherlands

    C. H. Schröder & L. A. H. Monnens

  3. Department of Pathology, University of Nijmegen, P. O. B. 9101, NL-6500 HB, Nijmegen, The Netherlands

    K. J. M. Assmann

  4. Renal Unit, Hammersmith Hospital, University of London, London, UK

    C. O. S. Savage

  5. Department of Paediatrics, University of Amsterdam, Amsterdam, The Netherlands

    D. Menzel

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  1. L. P. W. J. v. d. Heuvel
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  2. C. H. Schröder
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  3. C. O. S. Savage
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  6. L. A. H. Monnens
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  7. J. H. Veerkamp
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v. d. Heuvel, L.P.W.J., Schröder, C.H., Savage, C.O.S. et al. The development of anti-glomerular basement membrane nephritis in two children with Alport's syndrome after renal transplantation: characterization of the antibody target. Pediatr Nephrol 3, 406–413 (1989). https://doi.org/10.1007/BF00850217

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  • DOI: https://doi.org/10.1007/BF00850217

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