Summary
The role of stretching-generated tensile stresses upon the organization of axial rudiments have been studied. Pieces of the dorsal wall ofXenopus laevis andRana temporaria embryos at the late gastrula stage were rotated through 90°, transplanted into the field of neurulation tensions of another embryo and replaced by ventral tissues already insensitive to inductive influences. The axial rudiments which developed from rotated and transplanted dorsal tissues oped from rotated and transplanted dorsal tissues almost completely reorientated according to the tensile patterns in adjacent host tissues. Some of the donor cells changed their presumptive fates in accordance with their new positions in the host tensile field. Transplanted ventral tissues were involved in the morphogenetic movements specific for the dorsal regions and imitated some typical dorsal structures. In the regions without pronounced tensions the structure of transplanted axial rudiments was chaotic. It is suggested that the organization of the axial structures is established and maintained by tensile fields created by uniformly polarized cells. Cell polarization can be transmitted by contact from host to donor tissues. The specificity of this propagating process and its morphogenetical role is discussed.
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Beloussov, LV (1978): Formation and cellular structure of tension lines in axial rudiments of the amphibian embryos (in Russian). Ontogenez 9:124–130
Beloussov, LV (1979) Experiments on alterations of tensile fields in the axial rudiments of amphibian embryos (in Russian). Ontogenez 10:120–129
Beloussov, LV, Dorfman JG, Cherdantzev VG (1975) Mechanical stresses and morphological patterns in amphibian embryos. J Embryol Exp Morphol 34:559–574
Cambar, R, Marrot, B (1954) Table chronologique du development de la grenoille agile (Rana dalmatina Bon.). Bull Biol France-Belgique, 88:168–177
Cherdantzev, VG (1977) Spatial deploiments of morphogenetic movements as elements of the oral field in Anura (in Russian). Ontogenez 8:335–360
Gierer A, Meinhardt, H (1972) A theory of biological pattern formation. Kybernetik 12:30–39
Goodwin, BC, Cohen MH (1969) A phase shift model for the spatial and temporal organization in developing systems. J Theor Biol 25:49–107
Jacobson, AG, Gordon, R (1976) Changes in the shape of the developing vertabrate nervous system analysed experimentally, mathematically and by computer simulation. J Exp Zool 197:191–246
Jacobson, C, Löfberg, J (1969) Mesoderm movements in the amphibian neurula. Zool Bdr Uppsal 38:233–239
Keller, RE (1976) Vital dye mapping of the gastrula and neurula of Xenopus laevis, II Prospectivrbas and morphogenetic movements of the deep layer. Dev Biol 51:118–137
Keller, R (1978) Time-lapse cinematographic analysis of superficial cell behavior during and prior to gastrulation in Xenopus laevis. J Morphol 157:223–248
Lutchinskaia, NN, Beloussov, LV (1977) Electron microscopy study of the rapid morphogenetic processes in explants of embryonic tissues. Ontogenez 8:263–268
Middleton, CA (1977) The effects of cell-cell contact on the spreading of pigmented retina epithelial cells in a culture. Exp Cell Res 109:349–359
Nieuwkoop, PD, Faber J (1956) Normal table of Xenopus laevis (Daudin). North Holland, Amsterdam p 243
Saxen, L, Toivonen, S (1962) Primary embryonic induction. Logos/Academic Press, New York and London
Schroeder, T (1970) Neurulation inXenopus laevis. An analysis and model based upon light and electron microscopy. J. Embryol Exp Morphol 23:427–462
Slack, J (1978) Chemical waves in Drosophila. Nature 271:403–404
Tickle, C, Trinkaus, JP (1976) Observations on nudging cells in culture. Nature 261:413
Trinkaus, JP: Mediation of cell surface behavior by intercellular contact. Zoon 6:51–63
Zavalishina, LE, Beloussov, LV, Ostroumova, TV (1980) The relations between contact polarization of emphibian embryonic cells and the rate of protein synthesis. Ontogenez 11: in press
Zeeman, EC (1974) Primary and secondary waves in developmental biology. Lect Math Life Sci Am Math Soc 7:69–161
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Beloussov, L.V. The role of tensile fields and contact cell polarization in the morphogenesis of amphibian axial rudiments. Wilhelm Roux' Archiv 188, 1–7 (1980). https://doi.org/10.1007/BF00848603
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DOI: https://doi.org/10.1007/BF00848603