Summary
Gastric phenotypic expression indicated by paradoxical concanavalin A (Con A) staining for class III mucins and the immunoperoxidase method for pepsinogen (Pg) I and Pg II was found in pyloric gland metaplasia of gallbladder epithelium. Using the same methods, the features of gallbladder cancers and their relationship to pyloric gland metaplasia in the human gallbladder epithelium were studied. Histologically, 57 gallbladder cancers were classified into 5 papillary adenocarcinomas, 29 tubular adenocarcinomas, 8 poorly differentiated adenocarcinomas, 6 signet-ring cell carcinomas, 4 mucinous adenocarcinomas, and 5 squamous cell carcinomas. In papillary and tubular adenocarcinomas, Pg I and/or Pg II staining was detected in 80% and 75.9% of cancers, respectively. Pg II staining was significantly more frequent than Pg I staining. One signetring cell carcinoma also had Pg II activity. Pyloric gland metaplasias all contained class III mucins and were further classified into complete type and incomplete type on the basis of presence or absence Pg I and/ or Pg II activities. A few cancer cells with class III mucins were negative for Pg staining; conversely, a few cells with Pg I and/or Pg II had no class III mucins. Phenotypic diversity in both class III mucin reactivity and Pg activities was observed in gallbladder cancer cells with the pyloric gland cell type. By comparison, pyloric gland metaplasia varied only in Pg activities. A few Pg-positive cancers were found in the gallbladder with Pg-negative pyloric gland metaplasia. The present results clearly indicate the appearance of gastric phenotypic expression in both gallbladder epithelium and gallbladder cancers and suggest the independent induction of pyloric gland metaplasia and cancer with gastric phenotypic expression.
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References
Albores-Saavedra J, Nadji M, Henson DE (1986) Intestinal type adenocarcinoma of the gallbladder: A clinicopathologic and immunocytochemical study of seven cases. Am J Surg Pathol 10:19
Furihata C, Saito D, Fujiki H, Kanai Y, Matsushima T, Sugimura T (1980) Purification and characterization of pepsinogens and a unique pepsin from rat stomach. Eur J Biochem 105:43–50
Furihata C, Tatematsu M, Miki K, Katsuyama T, Sudo K, Miyagi N, Kubota T, Jin S, Kodama K, Ito N, Konishi Y, Suzuki K, Matsushima T (1984) Gastric- and intestinal-type properties of human gastric cancers transplanted into nude mice. Cancer Res 44:727–733
Furihata C, Tatematsu M, Shirai T, Yokochi K, Takahashi M, Sugimura T (1976) Pepsinogens and stomach cancer. In: Farber E, Kawachi T, Nagayo T (eds) Pathophysiology of carcinogenesis in digestive organs. University of Tokyo Press, Tokyo, pp 49–63
Graham RCJR, Karnofsky MJ (1966) The early stage of absorption of injected horse radish peroxidase in the proximal convoluted tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Hirai S (1980) Clinicopathological study on metaplasia in resected gallbladder: As a background of histogenesis of gallbladder cancer. Jpn J Gastroenterol Surg 13:35–44
Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Ichinose M, Miki K, Furihata C, Kageyama T, Niwa H, Oka H, Oda T, Matsushima T, Takahashi K (1982) Radioimmunoassay of group II pepsinogen in human serum. Clin Chim Acta 122:61–69
Katsuyama T, Spicer SS (1978) Histochemical differentiation of complex carbohydrates with variants of the concanavalin A-horse radish peroxidase method. J Histochem Cytochem 26:233–250
Kozuka S, Tsubone M, Yasui A, Hachisuka K (1982) Relation of adenoma to carcinoma in the gallbladder. Cancer 50:2226–2234
Meuwissen SG, Mullink H, Bosma A, Pals G, Defize J, Flipse M, Westerveld BD, Tas M, Brakke J, Kreuning J, Eriksson AW, Meyer CJLM (1984) Immunocytochemical localization of pepsinogen I and II in the human stomach. In: Kreuning J, Samloff IM, Rotter JI, Eriksson AW (eds) Pepsinogens in man; clinical genetic advance. Aalsmeer, Mur-Kostverloren, pp 123–130
Nakamura K, Sugano H, Takagi K (1968) Carcinoma of the stomach in incipient phase: its histogenesis and histological appearance. Gann 59:251–258
Samloff IM (1969) Slow moving protease and the seven pepsinogens. Electrophoretic demonstration of the existence of eight proteolytic fractions in human gastric mucosa. Gastroenterology 67:659–669
Samloff IM (1971) Cellular localization of group I pepsinogens in human gastric mucosa by immunofluorescence. Gastroenterology 61:185
Samloff IM, Liebman WM (1973) Cellular localization of the group II pepsinogens in human stomach and duodenum by immunofluorescence. Gastroenterology 65:36–42
Suganuma T, Katsuyama T, Tsukahara M, Tatematsu M, Sakakura Y, Murata F (1981) Comparative histochemical study of alimentary tracts with special reference to the mucous neck cells of the stomach. Am J Anat 161:219–238
Tatematsu M, Furihata C, Katsuyama T, Hasegawa R, Nakanowatari J, Saito D, Takahashi M, Matsushima T, Ito N (1983) Independent induction of intestinal metaplasia and gastric cancer in rats treated with N-methyl-N′-nitro-N-nitrosoguanidine. Cancer Res 43:1335–1341
Tatematsu M, Furihata C, Katsuyama T, Miki K, Honda H, Konishi Y, Ito N (1986) Gastric and intestinal phenotypic expressions of human signet ring cell carcinomas revealed by their biochemistry, mucin histochemistry, and ultrastructure. Cancer Res 46:4866–4872
Tatematsu M, Furihata C, Katsuyama T, Mera Y, Inoue T, Matsushima T, Ito N (1987a) Immunohistochemical demonstration of pyloric gland-type cells with low-pepsinogen isozyme 1 (Pg 1) in preneoplastic and neoplastic tissues of rat stomachs treated with N-methyl-N′-nitro-N-nitrosoguanidine. J Natl Cancer Inst 78:771–777
Tatematsu M, Furihata C, Miki K, Ichinose M, Shirai T, Tatematsu K, Ito N (1987b) Complete and incomplete pyloric gland metaplasia of human gallbladder. Acta Pathol Jpn 37:39–46
Tatematsu M, Katsuyama T, Fukushima S, Takahashi M, Shirai T, Nasu T (1980) Mucin histochemistry by paradoxical concanavalin A staining in experimental gastric cancers induced in Wistar rats by N-methyl-N′-nitro-N-nitrosoguanidine or 4-nitroquinoline 1-oxide. J Natl Cancer Inst 64:835–843
Tatematsu M, Katsuyama T, Furihata C, Tsuda H, Ito N (1984) Stable intestinal phenotypic expression of gastric and small intestinal tumor cells induced by N-methyl-N′-nitro-N-nitrosoguanidine or methylnitrosourea in rats. Gann 75:957–965
Tsutsumi Y, Nagura H, Osamura Y, Watanabe K, Yanaihara N (1984) Histochemical studies of metaplastic lesions in the human gallbladder. Arch Pathol Lab Med 108:917–921
Yamagiwa H, Tomiyama H (1986) Intestinal metaplasia-dysplasia-carcinoma sequence of the gallbladder. Acta Pathol Jpn 36:989–997
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Tatematsu, M., Ichinose, M., Miki, K. et al. Gastric phenotypic expression in human gallbladder cancers revealed by pepsinogen immunohistochemistry and mucin histochemistry. Vichows Archiv A Pathol Anat 413, 25–32 (1988). https://doi.org/10.1007/BF00844278
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DOI: https://doi.org/10.1007/BF00844278