Abstract
The effect of heparin, spermidine, and Be2+ ions on adenosine triphosphatase (ATPase), β-glycerophosphatase, and ribonuclease (RNase) activity of rat liver cell nuclei was investigated in vitro. Be2+ ions were shown to inhibit ATPase and β-glycerophosphate activity, the former more effectively. Heparin and spermidine, in physiological concentrations, also inhibited the activity of both phosphatases. Heparin and spermidine had a similar action on nuclear RNase activity. The inhibitory effect of these agents on endonucleases was revealed.
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Literature Cited
L. N. Delektorskaya and K. A. Perevoshchikova, Biokhimiya,34, 199 (1969).
I. B. Zbarskii, in: Cell Nucleus [in Russian], Moscow (1972), pp. 229–243.
Ya. M. Koen, K. A. Perevoshchikova, and I. B. Zbarskii, Biokhimiya,41, 982 (1976).
E. G. Rozet, N. I. Teterev, L. F. Panchenko, et al., Dokl. Akad. Nauk SSSR,216, 1188 (1974).
A. D. Skridonenko, K. A. Perevoshchikova, and I. B. Zbarskii, Biokhimiya,40, 384 (1975).
P. S. Agutter, J. J. McArdle, and B. McCaldin, Nature,263, 165 (1976).
I. B. Buchwalow and E. Unger, Exp. Cell Res.,106, 139 (1977).
L. S. Cutler, A. P. Chaudhry, and M. Montes, J. Histochem. Cytochem.,22, 1113 (1974).
G. P. Georgiev, O. P. Samarina, M. I. Lerman, et al., Nature,200, 1291 (1963).
R. M. Hays and P. Barland, J. Cell Biol.,31, 209 (1966).
N. Hazan and R. McCauley, Biochem. J.,156, 665 (1976).
K. Igarashi, Y. Watanabe, H. Kumagai, et al., J. Biochem. (Tokyo),81, 579 (1977).
O. H. Lowry, N. J. Rosebrough, A. L. Farr, et al., J. Biol. Chem.,193, 265 (1951).
R. K. Morton, Methods in Enzymology, Vol. 2, New York (1955), pp. 533–539.
A. O. Pogo, V. G. Allfrey, and A. E. Mirsky, Proc. Natl. Acad. Sci. USA,56, 550 (1966).
J. Racevskis and T. E. Webb, Eur. J. Biochem.,49, 93 (1974).
J. S. Roth, J. Biol. Chem.,231, 1085 (1958).
T. Sato, K. Ishikawa, and K. Ogata, Biochim. Biophys. Acta,474, 549 (1977).
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Laboratory of Biochemistry, N. K. Kol'tsov Institute of Developmental Biology, Academy of Sciences of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 87, No. 6, pp. 542–544, June, 1979.
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Perevoshchikova, K.A., Prokop, K., Hering, B. et al. Effect of heparin, spermidine, and Be2+ ions on phosphatase and ribonuclease activity of rat liver cell nuclei. Bull Exp Biol Med 87, 565–568 (1979). https://doi.org/10.1007/BF00801386
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DOI: https://doi.org/10.1007/BF00801386