Summary
The effects of the availabilities of water and nitrogen on water use efficiency (WUE) of plants were investigated in a sagebrush steppe. The four species studied wereArtemisia tridentata (shrub),Ceratoides lanata (suffrutescent shrub),Elymus lanceolatus (rhizomatous grass), andElymus elymoides (tussock grass). Water and nitrogen levels were manipulated in a two-by-two factorial design resulting in four treatments: control (no additions), added water, added nitrogen, and added water and nitrogen. One instantaneous and two long-term indicators of WUE were used to testa priori predictions of the ranking of WUE among treatments. The short-term indicator was the instantaneous ratio of assimilation to transpiration (A/E). The long-term measures were 1) the slope of the relationship between conductance to water vapor and maximum assimilation and 2) the carbon isotope composition (δ13C) of plant material. Additional water decreased WUE, whereas additional nitrogen increased WUE. For both A/E and δ13C, the mean for added nitrogen alone was significantly greater than the mean for added water alone, and means for the control and added water and nitrogen fell in between. This ranking of WUE supported the hypothesis that both water and nitrogen limit plant gas exchange in this semiarid environment. The short- and long-term indicators were in agreement, providing evidence in support of theoretical models concerning the water cost of carbon assimilation.
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Anderson JE, Shumar ML, Toft NL, Nowak RS (1987) Control of the soil water balance by sagebrush and three perennial grasses in a cold-desert environment. Arid Soil Res Rehab 1:229–244
Barkworth ME, Dewey DR, Atkins RJ (1983) New generic concepts in the Triticeae of the Intermountain Region: key and comments. Great Basin Nat 43:561–572
Blackman PG, Davies WJ (1985) Root to shoot communication in maize plants of the effects of soil drying. J Exp Bot 36:39–48
Bolton JK, Brown RH (1980) Photosynthesis of grass species differing in carbon dioxide fixation pathways. V. Response ofPanicum maximum, Panicum milioides, and tall fescue (Fescue arundinacea) to nitrogen nutrition. Plant Physiol 66:97–100
Bradford KJ, Sharkey TD, Farquhar GD (1983) Gas exchange, stomatal behavior, and δ13C values of theflacca tomato mutant in relation to abscisic acid. Plant Physiol 72:245–250
Briggs GM, Jurik TW, Gates DM (1986) Non-stomatal limitation of CO2 assimilation in three tree species during natural drought conditions. Physiol Plant 66:521–526
Brown RH, Wilson JR (1983) Nitrogen response ofPanicum species differing in CO2 fixation pathways. II. CO2 exchange characteristics. Crop Sci 23:1154–1159
Caemmerer S von, Farquhar GD (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153:376–387
Caldwell M (1985) Cold desert. In: Chabot BF, Mooney HA (eds) Physiological ecology of North American plant communities. Chapman and Hall, New York London, pp 198–212
Comstock J, Ehleringer J (1984) Photosynthetic responses to slowly decreasing leaf water potentials inEncelia frutescens. Oecologia 61:241–248
Cowan IR (1982) Regulation of water use in relation to carbon gain in higher plants. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Physiological plant ecology II. (Encyclopedia of plant physiology, NS, vol 12B). Springer, Berlin Heidelberg New York, pp 589–613
Cowan IR, Farquhar GD (1977) Stomatal function in relation to leaf metabolism and environment. Symp Soc Exp Biol 31:471–505
Ehleringer JR, Cooper TA (1988) Correlations between carbon isotope ratio and microhabitat in desert plants. Oecologia 76:562–566
Ehleringer JR, Cook CS, Tieszen LL (1986) Comparative water use and nitrogen relationships in a mistletoe and its host. Oecologia 68:279–284
Evans JR, Sharkey TD, Berry JA, Farquhar GD (1986) Carbon isotope discrimination measured concurrently with gas exchange to investigate CO2 diffusion in leaves of higher plants. Aust J Plant Physiol 13:281–292
Farquhar GD (1979) Carbon assimilation in relation to transpiration and fluxes of ammonia. In: Marcelle R, Clijsters H, Poucke M van (eds) Photosynthesis and Plant Development. Junk, The Hague, pp 321–328
Farquhar GD, Richards RA (1984) Isotopic composition of plant carbon correlates with water-use efficiency of wheat genotypes. Aust J Plant Physiol 11:539–552
Farquhar GD, Ball MC, Caemmerer S von, Roksandic Z (1982a) Effect of salinity and humidity on δ13C value of halophytes — Evidence for diffusional isotope fractionation determined by the ratio of intercellular/atmospheric partial pressure of CO2 under different environmental conditions. Oecologia 52:121–124
Farquhar GD, O'Leary MH, Berry JA (1982b) On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves. Aust J Plant Physiol 9:121–137
Field C, Berry JA, Mooney HA (1982) A portable system for measuring carbon dioxide and water vapor exchange of leaves. Plant Cell Environ 5:179–186
Forseth IN, Ehleringer JR (1983) Ecophysiology of two solar tracking desert winter annuals. III. Gas exchange responses to light, CO2 and VPD in relation to long-term drought. Oecologia 57:344–351
Gebauer G, Schuhmacher MI, Krstic B, Rehder H, Ziegler H (1987) Biomass production and nitrate metabolism ofAtriplex hortensis L. (C3 plant) andAmaranthus retroflexus L. (C4 plant) in cultures at different levels of nitrogen supply. Oecologia 72:303–314
Gollan T, Turner NC, Schulze E-D (1985) The responses of stomata and leaf gas exchange to vapour pressure deficits and soil water content. III. In the sclerophyllous woody speciesNerium oleander. Oecologia 65:356–362
Hitchcock CL, Cronquist A (1973) Flora of the Pacific Northwest. University of Washington Press, Seattle
Hubick KT, Farquhar GD, Shorter R (1986) Correlation between water-use efficiency and carbon isotope discrimination in diverse peanut (Arachis) germplasm. Aust J Plant Physiol 13:803–816
Jones MM, Rawson HM (1979) Influence of rate of development of leaf water deficits upon photosynthesis, leaf conductance, water use efficiency, and osmotic potential in sorghum. Physiol Plant 45:103–111
Longstreth DJ, Nobel PS (1980) Nutrient influences on leaf photosynthesis. Effects of nitrogen, phosphorus, and potassium forGossypium hirsutum L. Plant Physiol 65:541–543
Medina E (1972) Photosynthetic capacity and carboxydismutase activity inAtriplex patula leaves as determined by nitrogen nutrition and light intensity during growth. In: Forti G, Buron M, Metzner H (eds) Proc 2nd Int Congr Photosynth Res. Junk, The Hague, pp 2527–2536
Morgan JA (1986) The effects of N nutrition on the water relations and gas exchange characteristics of wheat (Triticum aestivum L.). Plant Physiol 80:52–58
Rawson HM (1979) Vertical wilting and photosynthesis, transpiration, and water use efficiency of sunflower leaves. Aust J Plant Physiol 6:109–120
Sage RF, Pearcy RW (1987) The nitrogen use efficiency of C3 and C4 plants. II. Leaf nitrogen effects on the gas exchange characteristics ofChenopodium album (L.) andAmaranthus retroflexus (L.). Plant Physiol 84:954–958
SAS Institute (1982) SAS User's Guide: Statistics. SAS Institute Inc, Cary, North Carolina
Schulze E-D, Hall AE (1982) Stomatal responses, water loss and CO2 assimilation rates of plants in contrasting environments. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Physiological plant ecology II. (Encyclopedia of plant physiology, NS, vol 12B). Springer, Berlin Heidelberg New York, pp 181–230
Sobrado MA, Turner NC (1986) Photosynthesis, dry matter accumulation and distribution in the wild sunflowerHelianthus petiolaris and the cultivated sunflowerHelianthus annuus as influenced by water deficits. Oecologia 69:181–187
West NE (1983) Western intermountain sagebrush steppe. In: West NE (ed) Temperate deserts and semideserts. Ecosystems of the world, vol 5. Elsevier, Amsterdam, pp 351–374
Wong S-C (1979) Elevated atmospheric partial pressure of CO2 and plant growth. Interactions of nitrogen nutrition and photosynthetic capacity in C3 and C4 plants. Oecologia 44:68–74
Wong S-C, Cowan IR, Farquhar GD (1979) Stomatal conductance correlates with photosynthetic capacity. Nature 282:424–426
Zur B, Jones JW (1984) Diurnal changes in the instantaneous water use efficiency of a soybean crop. Agr For Met 33:41–51
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Toft, N.L., Anderson, J.E. & Nowak, R.S. Water use efficiency and carbon isotope composition of plants in a cold desert environment. Oecologia 80, 11–18 (1989). https://doi.org/10.1007/BF00789925
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DOI: https://doi.org/10.1007/BF00789925